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S100A4 silencing blocks invasive ability of esophageal squamous cell carcinoma cells

World Journal of Gastroenterology 2012年第9期18卷 915-922页

作者： Dong Chen Xue-Feng Zheng Ze-You Yang Dong-Xiao Liu Guo-You Zhang Xue-Long Jiao Hui Zhao Department of Surgery the Affiliated Hospital of Qingdao Medi-cal CollegeQingdao UniversityQingdao 266003ShandongProvinceChina Department ofSurgery Tianjin Union Medicine CenterTianjin 300121China

AIM： To investigate a potential role of S100A4 in esoph- agus squamous cell carcinoma metastasis （ESCCs）.METHODS： Expression of $100A4 and E-cadherin were analyzed in frozen sections from ESCCs （metastasis, n = 28; non-metastasis, n = 20） by reverse transcrip- tion-polymerase chain reaction, quantitative polymerase chain reaction and immunohistochemistry. To explore the influence of $100A4 on esophageal cancer invasion and metastasis, $100A4 was overexpressed or silenced by $100A4 siRNA in TE-13 or Eca-109 cells/n vitro and /n vivo.

关键词： Esophagus squamous cell carcinoma metas-tasis Gene treatment $100A4 E-cadherin

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Tumors with macroscopic bile duct thrombi in non-HCC patients:Dynamic multi-phase MSCT findings

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World Journal of Gastroenterology 2012年第11期18卷 1273-1278页

作者： Qing-Yu Liu Xiao-Feng Lin Hal-Gang Li Ming Gao Wei-Dong Zhang Department of Radi-ology Sun Yat-Sen Memorial Hospital Sun Yat-Sen UniversityGuangzhou 510120 Guangdong Province China Department of Pathology Sun Yat-Sen MemorialHospital Sun Yat-Sen University Guangzhou 510120 Guang-dong Province China Department of Radiology Cancer CenterSun Yat-Sen University Guangzhou 510060 Guangdong Prov-ince China

Non-hepatocellular carcinoma （non-HCC） with macroscopic bile duct tumor thrombus （BDTI） formation is rare, few radiological studies have been reported. In this case report, we retrospectively analyzed the imaging findings of three cases of non-HCC with macroscopic BDTT on dynamic enhanced multislice computed tomography （MSCT） scan. One case of primary hepatic carcinosarcoma was presented as a solitary, large welldefined tumor with significant necrotic changes. One case of liver metastasis from colon cancer was presented as a Iobulated, large ill-defined tumor. One case of intraductal oncocytic papillary neoplasm involved the entire pancreas, presented as a cystic and solid mass with multilocular changes （the individual Ioculi were less than 5.0 mm in diameter）. The bile duct was dilated due to expansible growth of the BDFE in all three patients. The BDTT was contiguous with hepatic or pancreatictumor, and both of them showed the same enhancement patterns on dynamic contrast-enhanced computed tomography scan： early enhancement in the hepatic arterial phase and a quick wash out of contrast agent in the portal and equilibrium phases. Macroscopic BDTT- in non-HCC patient is rare, dynamic enhanced MSCT scan may be valuable in the diagnosis of non-HCC with BDTT.

关键词： Liver neoplasms Carcinosarcoma metas-tasis Pancreatic neoplasms Oncocytic papillary neo-plasm Bile ducts tumor thrombus Computed tomog-raphy X-ray

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Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells

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World Journal of Gastroenterology 2013年第46期19卷 8764-8769页

作者： Feng-Lan Gao Wei-Shan Li Chun-Ling Liu Guo-Qiang Zhao Pathological Examination and Research Center Luohe Medical College Basic Medical Science Institute Zhengzhou University

AIM:To evaluate the impact of Bmi-1 on cell senescence and metastasis of human gastric cancer cell line ***:Two pairs of complementary small hairpin RNA(shRNA)oligonucleotides targeting the Bmi-1gene were designed,synthesized,annealed and cloned into the pRNAT-U6.2 *** DNA sequencing to verify the correct insertion of the shRNA sequences,the recombinant plasmids were transfected into BGC823 *** expression of Bmi-1 mRNA and protein was examined by reverse transcription-polymerase chain reaction(RT-PCR)and Western *** effects of Bmi-1 knockdown on cell senescence and metastasis were determined by theβ-Gal activity assay and Boyden chamber assay,***:The double-stranded oligonucleotide fragments of Bmi-1 short interfering RNA(siRNA)cloned into pRNAT-U6.2 vector conformed to the inserted ***-PCR and Western blotting indicated that the expression levels of Bmi-1 gene mRNA and protein were markedly decreased in transfected BGC823 cells with pRNAT-U6.2-si1104 and pRNATU6.2-si1356,especially in transfected BGC823 cells with pRNAT-U6.2-si1104,compared with two control groups(empty vector and blank group).In particular,Bmi-1 protein expression was almost completely abolished in cells transfected with the recombinant vector harboring shRNA targeting the sequence GGAGGAGGTGAATGATAAA(nt1104-1122).Compared with untransfected cells and cells transfected with the empty vector,the mean percentage of senescent cells increased and the number of cells passing through the Matrigel decreased in cells transfected with the recombinant ***:Silencing Bmi-1 by RNA interference can increase the senescent cell rate and effectively reduce the metastasis of gastric cancer cells.

关键词： Bmi-1 Gastric cancer Senescence metas-tasis

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Prognosis of patients with gastric cancer and solitary lymph node metastasis

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World Journal of Gastroenterology 2013年第46期19卷 8611-8618页

作者： Chun-Qiu Chen Xiao-Jiang Wu Zhen Yu Zhao-De Bu Ke-Qiang Zuo Zi-Yu Li Jia-Fu Ji Department of General Surgery Tenth People’s Hospital of Shanghai Tongji University School of Medicine Department of Surgery Beijing Cancer Hospital Peking University School of Oncology

AIM:To investigate the relationship of solitary lymph node metastasis(SLNM)and age with patient survival in gastric cancer(GC).METHODS:The medical records databases of China’s Beijing Cancer Hospital at the Peking University School of Oncology and Shanghai Tenth People’s Hospital affiliated to Tongji University were searched retrospectively to identify patients with histologically proven GC and SLNM who underwent surgical resection between October 2003 and December *** with distant metastasis or gastric stump carcinoma following resection for benign disease were excluded from the *** total,936 patients with GC+SLNM were selected for analysis and the recorded parameters of clinicopathological disease and follow-up(range:13-2925 d)were *** Kaplan-Meier method was used to stratify patients by age(≤50 years-old,n=198;50-64 years-old,n=321;≥65 years-old,n=446)and by metastatic lymph node ratio[MLR<0.04(1/25),n=180;0.04-0.06(1/25-1/15),n=687;≥0.06(1/15),n=98]for 5-year survival *** significance of intergroup differences between the survival curves was assessed by a log-rank test. RESULTS:The 5-year survival rate of the entire GC+SLNM patient population was 49.9%.Stratification analysis showed significant differences in survival time(post-operative days)according to age:≤50 yearsold:950.7±79.0 vs 50-64 years-old:1697.8±65.9 vs≥65 years-old:1996.2±57.6,all P<*** addition,younger age(≤50 years-old)correlated significantly with mean survival time(r=0.367,P<0.001).Stratification analysis also indicated an inverse relationship between increasing MLR and shorter survival time:<0.04:52.8%and 0.04-0.06:51.1%vs≥0.06:40.5%,P<*** patients with the shortest survival times and rates were younger and had a high MLR(≥0.06):≤50 years-old:496.4±133.0 and 0.0%vs 50-65 years-old:1180.9±201.8 and 21.4%vs≥65 years-old:1538.4±72.4 and 37.3%,all P<*** same significant trend in shorter survival times and rates for younger patients was seen with t

关键词： Gastric cancer Solitary lymph node metas-tasis Age metastatic lymph node ratio Survival

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