检索结果-南通市图书馆

metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius

Asian Pacific Journal of Tropical Biomedicine 2020年第8期10卷 353-360页

作者： Syed Ali Raza Ayoub Rashid Chaudhary Muhammad Waseem Mumtaz Ahmad Adnan Hamid Mukhtar Muhammad Tayyab Akhtar Department of Chemistry Government College UniversityLahore54000 Pakistan Department of Chemistry University of GujratGujrat Pakistan Institute of Industrial Biotechnology Government College UniversityLahore54000 Pakistan

Objective:To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus ***:The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted *** phenolic contents,flavonoid contents,antioxidant activity,α-glucosidase andα-amylase inhibitions of leaf extracts were *** metabolite profiling was accomplished by UHPLC-Q-TOF-MS/MS *** antidiabetic assessment of the most potent extract was carried out by measuring the hypoglycemic and hypolipidemic effect in the high fat diet-fed diabetic albino *** blood glucose level,haemoglobin,total cholesterol,high-density lipoproteins(HDL)and low-density lipoproteins(LDL)were ***:The 60%ethanolic extract exhibited the highest phenolic and flavonoid contents of(349.39±2.13)mg GAE/g dry extract and(116.95±2.34)mg RE/g dry extracts,respectively,and the highest DPPH scavenging activity with an IC50 value of(32.87±1.11)μg/*** IC50 values forα-glucosidase andα-amylase inhibitions were(38.64±0.93)μg/mL and(44.80±1.57)μg/mL,***-Q-TOF-MS/MS analysis confirmed the presence of gallic acid,ellagic acid,corilagin,kaempherol-3-O-rutinoside,caffeic acid derivative,isorhamnetin and galloyl derivatives in the 60%ethanolic *** extract at a dose of 450 mg/kg body weight reduced blood glucose level,total cholesterol,LDL and HDL,and increased haemoglobin in alloxan-induced diabetic mice,Conclusions:Conocarpus lancifolius leaves are proved as a good source of biologically functional metabolites and possess antidiabetic activity which may be further explored to treat diabetes.

关键词： Antioxidant Antidiabetic Conocarpus lancifolius metabolite profiling Diabetic mice model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

DMMIC derivatization-assisted liquid chromatography-mass spectrometry method for metabolite profiling of the glutathione anabolic pathway in esophageal cancer tissues and cells

引用

Journal of Pharmaceutical Analysis 2023年第11期13卷 1365-1373页

作者： Li Liu Yu-Han Lu Min-Dan Wang Qun-Fei Zhao Xiu-Ping Chen Hang Yin Chen-Guo Feng Fang Zhang The Research Center of Chiral Drugs Shanghai Frontiers Science Center for TCM Chemical BiologyInnovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghai201203China Department of Thoracic Surgery Renji HospitalSchool of MedicineShanghai Jiaotong UniversityShanghai200127China School of Public Health Shanghai University of Traditional Chinese MedicineShanghai201203China

In this work,a new pyrylium derivatization-assisted liquid chromatography-mass spectrometry(LC-MS)method was developed for metabolite profiling of the glutathione anabolic pathway(GAP)in cancer tissues and *** pyrylium salt of 6,7-dimethoxy-3-methyl isochromenylium tetrafluoroborate(DMMIC)was used to label the amino group of metabolites,and a reductant of dithiothreitol(DTT)was employed to stabilize the thiol *** combining DMMIC derivatization with LC-MS,it was feasible to quantify the 13 main metabolites on the GAP in complex biological samples,which had good linearity(R^(2)=0.99810.9999),precision(interday precision of 1.6%e19.0%and intraday precision of 1.4%e19.8%)and accuracy(83.4%-115.7%).Moreover,the recovery assessments in tissues(82.5%e107.3%)and in cells(98.1%e118.9%)with GSH-^(13)C2,^(15)N,and Cys-^(15)N demonstrated the reliability of the method in detecting tissues and *** a methodological evaluation,the method was applied successfully to investigate difference in the GAP between the carcinoma and para-carcinoma tissues of esophageal squamous cell carcinoma(ESCC)and the effect of p-hydroxycinnamaldehyde(CMSP)on the GAP in KYSE150 esophageal cancer *** results demonstrate that the developed method provides a promising new tool to elucidate the roles of GAP in physiological and pathological processes,which can contribute to research on drugs and diseases.

关键词： Glutathione anabolic pathway metabolite profiling DMMIC derivatization LC-MS Esophageal squamous cell carcinoma p-Hydroxycinnamaldehyde KYSE-150cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract

引用

Asian Pacific Journal of Tropical Biomedicine 2022年第4期12卷 164-174页

作者： Zain Ul Aabideen Muhammad Waseem Mumtaz Muhammad Tayyab Akhtar Muhammad Asam Raza Hamid Mukhtar Ahmad Irfan Syed Ali Raza Muhammad Nadeem Yee Soon Ling Department of Chemistry University of GujratGujratPakistan Institute of Industrial Biotechnology GC University LahoreLahorePakistan Department of Chemistry College of ScienceKing Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Research Center for Advanced Materials Science King Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Department of Chemistry GC University LahoreLahorePakistan Sabah Occupational Safety Health and Environment AssociationLot331st FloorBlock EIramanis CenterJalanLinatas88450Kota KinabaluSabahMalaysia

Objective:To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum ***:Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity *** ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum *** studies were performed using molecular docking and electrostatic potential analysis for individual *** hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich ***:The 80%hydroethanolic extract exhibited the highest extract yield,total phenolic contents,total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity,total antioxidant power,and pancreatic lipase inhibitory *** 80%hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver,kidney,and *** 80%hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in *** effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug ***:Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation.

关键词： Solanum nigrum Ultrasonication metabolite profiling Total phenolic contents Total flavonoid content Antioxidant DPPH Total antioxidant power Pancreatic lipase UHPLC-QTOF-MS/MS Antiobesity Mice Hypolipidemic Molecular docking

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Deciphering Starch Quality of Rice Kernels Using metabolite profiling and Pedigree Network Analysis

引用

Molecular Plant 2012年第2期5卷 442-451页

作者： Miyako Kusano Atsushi Fukushima Naoko Fujita Yozo Okazaki Makoto Kobayashi Naoko Fujita Oitome Kaworu Ebana Kazuki Saito RIKEN Plant Science Center Tsurumi Yokohama 230-0045 Japan KIHARA Institute for Biological Research Yokohama City University Totsuka Yokohama 244-0813 Japan Department of Biological Production Akita Prefectural University Akita 010-0195 Japan National Institute of Agrobiological Sciences 2-1-2 Kannondai Tsukuba Ibaraki 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Chiba 260-8675 Japan

The physiological properties of rice grains are immediately obvious to consumers. High-coverage metabolomic characterization of the rice diversity research set predicted a negative correlation between fatty acid and lipid levels and amylose/total starch ratio （amylose ratio）, but the reason for this is unclear. To obtain new insight into the relationships among the visual phenotypes of rice kernels, starch granule structures, amylose ratios, and metabolite changes, we investigated the metabolite changes of five Japonica cultivars with various amylose ratios and two knockout mutants （e 1, a Starch synthase Ilia （SSIIla）-deficient mutant and the SSIIla/starch branching enzyme （BE） double-knockout mutant 4019） by using mass spectrometry-based metabolomics techniques. Scanning electron microscopy clearly showed that the two mutants had unusual starch granule structures. The metabolomic compositions of two cultivars with high amylose ratios （Hoshiyutaka and Yumetoiro） exhibited similar patterns, while that of the double-knockout mutant, which has an extremely high amylose ratio, differed. Rice pedigree network analysis of the cultivars and the mutants provided insight into the association between metabolic-trait properties and their underlying genetic basis in rice breeding in Japan. Multidimensional scaling analysis revealed that the Hoshiyutaka and Yumetoiro cultivars were Indica-like, yet they are classified as Japonica subpopulations. Exploring metabolomic traits is a powerful way to follow rice genetic traces and breeding history.

关键词： starch quality traits amylose ratio metabolite profiling pedigree networks rice.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and metabolites Regulated in Treated Cancer Cell Lines

引用

Rice science 2024年第4期31卷 449-462,I0027-I0030页

作者： Anuradha KUMARI Wusirika RAMAKRISHNA Department of Biochemistry Central University of PunjabPunjab 151401India

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

关键词： anticancer cytotoxicity metabolite profiling plant tissue culture rice callus suspension culture secondary metabolite

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of water deficiency on relationships between metabolism,physiology,biomass,and yield of upland cotton(Gossypium hirsutum L.)

引用

Journal of Arid Land 2018年第3期10卷 441-456页

作者： Tohir A BOZOROV Rustam M USMANOV YANG Honglan Shukhrat A HAMDULLAEV Sardorbek MUSAYEV Jaloliddin SHAVKIEV Saidgani NABIEV ZHANG Daoyuan Alisher A ABDULLAEV Institute of Genetics and Plants Experimental Biology Uzbek Academy of Sciences Key Lab of Biogeography and Bioresource in Arid Land Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Department of Civil and Environmental Engineering University of Connecticut

Drought is a common abiotic stress that considerably limits crop production. The objective of this study is to explore the influence of water deficiency on the yield, physiologic and metabolomic attributes in upland cotton cultivars（Gossypium hirsutum L）. Cotton cultivars, ＇Ishonch＇ and ＇Tashkent-6＇ were selected to study the relationships among their physiologic, metabolomic and yield attributes during water deficiency. Deficit irrigation was designed by modifying the traditional watering protocol to reduce water use. Results indicate that cotton cultivars respond differently to water deficit stress. Water deficit significantly influenced plant height, the number of internodes, and sympodial branches in both cultivars. However, yield components such as the number of bolls, boll seed, lint mass, and individual plant yield were significantly reduced only in ＇Tashkent-6＇. The leaf area decreased and the specific leaf weight increased in ＇Ishonch＇ under deficit irrigation conditions. However, ＇Tashkent-6＇ demonstrated significant water loss compared to ＇Ishonch＇, and both cultivars showed reduced transpiration rates. Untargeted metabolite profiles of leaves showed clear separation in ＇Ishonch＇, but not in ＇Tashkent-6＇ under deficit irrigation compared to full irrigation. The individual metabolites such as proline and galactinol showed strong association with yield under water deficit stress. Moreover, this study indicates that leaf area and transpiration intensity influence yield during water deficiency. In summary, the correlation among morpho-physiologic, metabolic, and yield components significantly varied between the two cultivars under water deficiency. The flowering stage was sensitive to water stress for both cultivars. The direct relationship between physiology, metabolism, and yield may be a useful selection criterion for determining candidate parents for cotton drought tolerance breeding.

关键词： irrigation cotton drought tolerance metabolite profiling yield

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Postmortem metabolite Profile Changes of Mud Crab(Scylla paramamosain)Under Different Storage Conditions

引用

Journal of Ocean University of China 2021年第3期20卷 608-618页

作者： ZHANG Limin GUO Liping MU Changkao YE Yangfang WANG Chunlin School of Marine Sciences Ningbo UniversityNingbo 315211China

Mud crab(Scylla paramamosain)is one of the most consumed crabs in ***,it is believed that dead mud crabs are not ***,we investigated the postmortem metabolite profile changes of the muscle and hepatopancreas of mud crab associated with storage method using nuclear magnetic resonance-based metabolomics coupled with multivariate data *** results showed that postmortem changes of trimethylamine,organic acids(lactate,formate,and succinate),as well as ATP and its breakdown products are closely affected by the storage *** metabolites can be used as indicators for monitoring loss of crab ***,dead mud crabs preserved an edible quality at least for 4 h at room temperature,12h on ice,and 16 d at−20℃,strongly suggesting that dead crab consumption quality should be determined by *** shouldn’t be discarded without considering the effects of different storage conditions on their freshness.

关键词： mud crab freshness metabolite profiling nuclear magnetic resonance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Morphological,phylogenetic and metabolite profile of Prorocentrum clipeus,a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

引用

Acta Oceanologica Sinica 2024年第8期43卷 128-141页

作者： Ruifang Wang Mengmeng Tong Shiwen Zhou Junjie Zheng Wenguang Zhang Xinfeng Dai Douding Lu Jiarong Hu Tianze Leng Qinglin Mu Zhongyong Yan Jiangning Zeng Pengbin Wang Ocean College Zhejiang UniversityZhoushan 316021China Key Laboratory of Marine Ecosystem Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China The State Key Laboratory of Marine Pollution Department of Biomedical SciencesCity University of Hong KongHong Kong SAR 999077China Zhejiang Marine Ecology and Environment Monitoring Center Zhoushan 316000China

More than 30 species of benthic Prorocentrum have been identified,some of which produce okadaic acid(OA)and its derivatives,dinophysistoxins(DTXs),which cause diarrhetic shellfish poisoning(DSP).Increasing numbers of benthic Prorocentrum species have been reported in tropical and subtropical waters of *** contrast,only a few benthic Prorocentrum species have been reported in temperate *** this study,morphological descriptions obtained using light microscopy,scanning electron microscopy and molecular characterization of one Prorocentrum clipeus strain isolated from the Yellow Sea are *** clipeus cells were nearly circular in shape,with a collar,ridge,and one *** periflagellar area was wide U-shaped,with two curved projections on platelet *** periflagellar platelets of different sizes were *** morphology closely fits that of the species isolated from other *** analysis based on the molecular sequences of the small subunit(SSU)rDNA,internal transcribed spacer(ITS),and large subunit(LSU)rDNA was performed.A comprehensive metabolomic analysis incorporating target,suspect and non-target screenings was first applied to investigate the intracellular and extracellular metabolite profiles of the current isolate of *** to the results of the target and suspect screenings,179 metabolites or toxins produced by DSP-related algal species,including OA,dinophysistoxin-1(DTX1),dinophysistoxin-2(DTX2)and pectenotoxin-2(PTX2),were not ***-target screening involving feature-based molecular networking(FBMN)provided a global view of major metabolites produced by the *** DF128 strain and revealed 23 clusters belonging to at least 13 compound classes,with organometallic compounds,lipids and lipid-like molecules,phenylpropanoids and polyketides,and benzenoids as major *** date,this is the first record of the characterization of *** in samples from Chinese *** results suppo

关键词： Prorocentrum taxonomy phylogeny metabolite profiling Yellow Sea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion

引用

Journal of Pharmaceutical Analysis 2021年第1期11卷 37-47页

作者： Barbara Bojko Nikita Looby Mariola Olkowicz Anna Roszkowska Bogumiła Kupcewicz Pedro Reck dos Santos Khaled Ramadan Shaf Keshavjee Thomas K.Waddell German Goomez-Rios Marcos Tascon Krzysztof Gorynski Marcelo Cypel Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooON M1B 6G3Canada Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Torun85-089BydgoszczPoland Jagiellonian Centre for Experimental Therapeutics(JCET) Jagiellonian University30-348 KrakowPoland Department of Pharmaceutical Chemistry Medical University of Gdansk80-416GdanskPoland Department of Inorganic and Analytical Chemistry Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Torun85-089BydgoszczPoland University Health Network-TGH TorontoON M5G 2C4Canada

Development of a novel in vivo lung perfusion(IVLP)procedure allows localized delivery of high-dose doxorubicin(DOX)for targeting residual micrometastatic disease in the ***,DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window.A small dimension nitinol wire coated with a sorbent of biocompatible morphology(Bio-SPME)has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key *** in vivo Bio-SPME-IVLP experiments were performed on pig model over various(150 and 225 mg/m^(2))drug doses,and during human clinical *** patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL(respectively)dose of DOX during a 3-h *** both pig and human cases,DOX tissue levels presented similar trends during *** lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP *** addition to DOX levels,Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening,providing information about lung status during drug ***-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy ***-SPME also extracted various endogenous molecules,thus providing a real-time snapshot of the physiology of the cells,which might assist in the tailoring of personalized treatment strategy.

关键词： In vivo solid phase microextraction metabolite profiling Spatial resolution Therapeutic drug monitoring Tissue analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)

引用

Asian Pacific Journal of Tropical Biomedicine 2018年第8期8卷 418-424页

作者： Sateesh Chandra Gupta Tusha Tripathi Shravan Kumar Paswan Annie G.Agarwal Chandana V.Rao Om P.Sidhu Department of Phytochemistry CSIR-National Botanical Research Institute Department of Pharmacology CSIR-National Botanical Research Institute

Objective: To undertake metabolite profiling of various plant parts of Citrullus colocynthis, and assess antioxidant and wound healing activities of fractions for therapeutical applications. Methods: Extracts from leaves, stem, root, fruit pulp and seeds were analyzed using gas chromatography-mass spectrometry and high performance liquid chromatography. Variation in antioxidant potential was assayed by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging assay. The extract with highest antioxidant potential was subjected on in-vivo wound healing activity using excision wound model. Results: metabolite profiling of Citrullus colocynthis identified 70 chemically diverse metabolites from different plant parts by using a combination of GC-MS and HPLC. Concentration of colocynthin, a principal active secondary metabolite, ranged from 3.15 mg/g dry weight to 242.00 mg/g dry weight, the lowest being in leaves and highest in fruit pulp. DPPH radical scavenging activity of free radical(IC50) ranged from 196.44 μg/mL in fruit pulp to 413.33 μg/mL in leaves tissues. Significant wound contraction and increase in hydroxyproline content of granulation tissue were observed with ointment formulated from methanolic extract of fruit pulp. Conclusions: The study indicates that the methanol extract of Citrullus colocynthis fruit pulp when applied topically may promote wound contraction in rat model attributable to the accumulation of colocynthin. The high quantity of colocynthin(242.00 mg/g dry weight) and substantial concentration of 2,4-di-tert butyl phenol(3.2%), squalene(4.2%) and δ-tocopherol(2.5%) make this plant to provide new opportunities for development of medicinal, nutraceutical and dietary supplements with optimized functionality.

关键词： metabolite profiling Citrullus colocynthis GC-MS Antioxidant Wound healing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：