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Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis

World Journal of Gastroenterology 2009年第5期15卷 570-577页

作者： Di Na Fu-Nan Liu Zhi-Feng Miao Zong-Min Du Hui-Mian Xu Department of Oncology The First Affiliated Hospital China Medical University Shenyang 110001 Liaoning Province China

AIM： To determine the inhibitory effect of Astragalus memebranaceushas on gastric cancer cell supernatant- induced apoptosis of human peritoneal mesothelial cells. METHODS： Human peritoneal mesothelial cell （HPMC） line HMrSV5 was co-incubated with gastric cancer cell supernatant （MKN45） and/or Astragalus memebranaceushas. Morphological changes in gastric cancer cells were observed under phase-contrast microscope. Quantitative cell damage was determined by MTT assay. Apoptosis was determined under transmission electron microscope and quantified by detectinr, acridine orange/ethidium bromide-stained （AO/EB） condensed nuclei under fluorescent microscope or by flow cytometry. Expressions of Bcl-2 and Bax were evaluated with immunostaining.

关键词： Peritoneal carcinomatosis Stomachneoplasm Astragalus plant mesothelial cell Apoptosis

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Destruction of Gastric Cancer cells to mesothelial cells by Apoptosis in the Early Peritoneal Metastasis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第2期29卷 163-168页

作者：那迪刘福囝苗志峰杜宗敏徐惠绵 Department of Oncology the First Affiliated HospitalChina Medical University

This study examined the mechanism by which the gastric cancer cells lead to early peritoneal metastasis. HMrSV5 cells, a human peritoneal mesothelial cell line, were co-incubated with the supernatants of gastric cancer cells. Morphological changes of HMrSV5 cells were observed. The cell damage was quantitatively determined by MTT assay. The apoptosis of HMrSV5 cells was observed under transmission electron microscope. Acridine orange/ethidium bromide-stained condensed nuclei was detected by fluorescent microscopy and flow cytometry. The expressions of Bcl-2 and Bax was immunochemically evaluated. The results showed that conspicuous morphological changes of apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. The supematants could induce apoptosis of HMrSV5 cells in a time-dependent manner. The supernatants could up-regulate the expression of Bax and suppress that of Bcl-2 in HMrSV5 cells. These findings demonstrated that gastric cancer cells can induce the apoptosis of HPMCs through supernatants in the early peritoneal metastasis, The abnormal expressions of Bcl-2 and Bax may contribute to the apoptosis. Anti-apoptosis drugs promise to be adjuvant chemotherapeutic agents in the treatment of peritoneal metastasis of gastric cancer.

关键词： peritoneal carcinomatosis stomach neoplasms mesothelial cell apoptosis

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