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Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro

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Journal of Pharmaceutical Analysis 2019年第4期9卷 284-291页

作者： Hanaa Rady Sohair Salem Mohamed Ez El-Arab Chemistry of Natural Compound Department National Research Centre Cairo Egypt Molecular Genetics and Enzymology Department National Research Centre Cairo Egypt National Institute of Oceanography and Fisheries (NIOF) Hurghada Egypt

Cancer recurrence and severe side effects of currently being used chemotherapeutic agents reduce their clinical efficacy. Thus, there is a constant need to develop alternative anticancer drugs. Sustainable supply is an important challenge facing marine-based drug discovery. Primmorph, a 3D cell culture system, could provide a sustainable source to produce metabolites for anticancer drugs from marine sponges. In the present work, the anticancer activity of primmorph extracts and mesohyls of Negombata magnifica, Hemimycle arabica, Crella spinulata, and Stylissa carteri sponges was evaluated. Antiproliferative activity was studied in terms of cytotoxicity, colony formation, cell cycle, and apoptosis. Migration was assessed by migration assay and matrix metalloproteinase activity. The expression of proliferation and migration-related genes was analyzed using real time PCR. Migration and proliferation activities of HepG2 cells were inhibited by treatment with primmorph extracts and mesohyls of N. magnifica, H. arabica, and C. spinulata. The mesohyl of S. carteri did not show any anticancer activity although the primmorph extract led to cell cycle arrest. Among the selected sponge species, the primmorph extract of C. spinulata was the most promising anticancer agent regarding antiproliferative and antimigratory activities. In addition, primmorph extracts have the advantage of working under welldefined and controlled conditions, which allows the easy application as a bioreactor.

关键词： Marine sponge Primmorph mesohyl Proliferation Migration Hepatocellular carcinoma cell line

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