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Expression of Pref-1 and Related Chemokines during the Development of Rat mesenteric lymph nodes

Biomedical and Environmental Sciences 2018年第7期31卷 507-514页

作者： PENG Yan JIA Li Min LI Bao Xin XIE Li Ping XIE Zun Jiang ZHENG Jin Hua Department of Anatomy Basic Medical Science College Harbin Medical University Department of Pharmacology Pharmacy College Harbin Medical University

Objective The aim of this study was to investigate the ability of Pref-1~＋ adipocyte progenitor cells to mobilize into mesenteric lymph nodes（MLNs） and the dynamic expression of related chemokines during the development of rat MLNs. Methods Immunohistochemical analyses were used to detect the expression of Pref-1 and related chemokines. Transmission electron microscopy（TEM） was used to observe the changes in ultrastructure of MLNs. Results Cells containing lipid droplets were found in all rat MLNs at embryonic day（E） 18.5, 2 and 6 weeks（w） after birth, and they were similar to fibroblastic reticular cells（FRCs） or follicular dendritic cells（FDCs） under TEM. Pref-1~＋ adipocyte progenitor cells were found in all MLNs. The expression level of Pref-1 was significantly increased at 2 w after birth and decreased at 6 w after birth. The tendency of Cxcl12 expression was consistent with that of Pref-1 and was positively correlated with the expression of Pref-1（P 〈 0.01; r = 0.897）. At E18.5, Cxcl13, and Ccr7 were significantly expressed in the MLN anlage, but the expression level of Ccl21 was low. The expression level of Cxcl13, Ccr7, and Ccl21 in MLN were significantly increased at 2 w after birth（P 〈 0.05）, while the expression of Ccr7 and Ccl21 were significantly decreased at 6 w after birth（P 〈 0.05）. Conclusion Adipocyte progenitor cells are involved in the rat MLNs development through differentiation into FRC and FDC. The expression of the relevant chemokines during the development of MLNs is dynamic and may be related to the maintenance of lymph nodes self-balance state.

关键词： mesenteric lymph nodes Development Rat Ultrastructure Adipocyte progenitor cells Chemokines

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Allicin,a major garlic constituent,ameliorates mesenteric lymph node dendritic cell-associated immunosuppression after trauma/hemorrhagic shock in rats

Allicin,a major garlic constituent,ameliorates mesenteric ly...

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第九届全国免疫学学术大会

作者： Yun Zhang Wei Wu Tao Xu Lin-Xiao Chen Fang-Fang Huang Xiao-Yan Miao Jian Zhang Lei Jin Wei Chen Tao Ma Feng Liang Xue-Li Bai Ting-Bo Liang 浙江大学医学院附属第二医院

Background:Allicin is believed to be the main component responsible for the biological activity of garlic,and is known to stimulate the functional activity of antigen presenting cells(APCs).The regulation of mesenteric lymph node dendritic cells(MLN-DCs)related immunosuppression may have therapeutic potential for intra-intestinal bacterial translocation induced by trauma/hemorrhagic shock(TH/S).This study therefore examined the effects of allicin on the function of MLN-DCs following T/HS in a Sprague-Dawley rat ***:Using an OX62 mAb,MLN-DCs were evaluated for surface expression of CD80,CD86,and MHCⅡ,as well as for *** also examined the in-vitro and in-vivo effects of allicin on MLN-DCs-induced induction of CD4T cell differentiation and the ***-intestinal bacterial translocation was monitored using bioluminescent citrobacter,and intestinal permeability tests were conducted using both FITC-Dextran and Ussing-Chember ***:The T/HS-induced increase in early apoptosis of MLN-DCs was ameliorated by allicin *** was likely mediated by caspase-3 and-9,AKT and ***80 and MHC-Ⅱexpression levels were downregulated in the T/HS group compared to the SHAM and allicin-treated SHAM groups,and upregulated after allicin ***,MLN-DCs from T/HS rats promoted differentiation of CD4+T-cells to Th2 and Treg *** partial amelioration of these trends by allicin treatment could be mediated by STAT1 and *** showed a significant increase in the permeability of the intestinal barrier after severe traumatic shock,along with an obvious intra-intestinal bacterial *** treatment significantly alleviated bacterial translocation,but had no influence on the permeability of the intestinal mechanical barrier in-vivo or ***:T/HS resulted in less functionally mature MLN-DCs and significant MLN-DCs related *** treatment ameliorated some of these altered MLN-DC functions as

关键词： Allicin dendritic cells mesenteric lymph nodes trauma/hemorrhagic shock

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Allicin, a major garlic constituent, ameliorates mesenteric lymph node dendritic cell-associated immunosuppression after trauma/hemorrhagic shock in rats

Allicin, a major garlic constituent, ameliorates mesenteric ...

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第十届全国免疫学学术大会

作者： Yun Zhang Tingbo Liang 浙江大学医学院附属第二医院

Background: Allicin is believed to be the main component responsible for the biological activity of garlic, and is known to stimulate the functional activity of antigen presenting cells(APCs). The regulation of mesenteric lymph node dendritic cells(MLN-DCs) related immunosuppression may have therapeutic potential for intra-intestinal bacterial translocation induced by trauma/hemorrhagic shock(TH/S). This study therefore examined the effects of allicin on the function of MLN-DCs following T/HS in a SD rat ***: Using OX62 m Ab, MLN-DCs were evaluated for surface expression of CD80, CD86, and MHC II, as well as for apoptosis. We also examined the in-vitro and in-vivo effects of allicin on MLN-DCs-induced induction of CD4 T cell differentiation. Intra-intestinal bacterial translocation was monitored using bioluminescent citrobacter, and intestinal permeability tests were conducted using both FITC-Dextran and Ussing-Chember assays. Results: The T/HS-induced increase in early apoptosis of MLN-DCs was ameliorated by allicin treatment. This was likely mediated by caspase-3 and-9. CD80 and MHC-II expression levels were downregulated in the T/HS group compared to the SHAM and allicin-treated SHAM groups, and upregulated after allicin treatment. Importantly, MLN-DCs from T/HS rats promoted differentiation of CD4T-cells to Th2 and Treg cells. The partial amelioration of these trends by allicin treatment could be mediated by STAT1 and STAT6. We showed a significant increase in the permeability of the intestinal barrier after traumatic shock, along with an obvious intra-intestinal bacterial translocation. Allicin treatment significantly alleviated bacterial translocation, but had no influence on the permeability of the intestinal mechanical ***: T/HS resulted in less functionally mature MLN-DCs and significant MLN-DCs related immunosuppression. Allicin treatment ameliorated some of these altered MLN-DC functions as well as the resulting intra-intestinal b

关键词： Allicin dendritic cells mesenteric lymph nodes trauma/hemorrhagic shock

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