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Anti-inflammatory effects of ethanol fraction from Disporum cantoniense （Lour.） merr. in LPS-stimulated RAW264. 7 cells

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中国药理学通报 2015年第B11期31卷 118-118页

作者： DONGLi DONGYong-xi ZHAOFei-fei WANGXia LIULi-na LIAOShang-gao LIYOng-jun GuizhouProvincialKeyLaboratoryofPharmaceutics SchoolofPharmacy GuizhouMedicalUniversityGuiyang550004

Aim To evaluate the anti-inflammatory effects of ethanol fraction prepared from Disporum cantoniense （Lour.） merr. 70% ethanol extract with a cellular model of LPS-stimulated RAW264.7 cell. Methods RAW264.7 cells were treated with different concentrations of ethanol fraction （25,50 and 100 g · L^-1 ） and stimu- the conditioned media was collected and analyzed. The quantity of ni- lated with LPS （10 μg· L^-1） for 24 hours, tric oxide （NO） was assayed by Griess reagent. The production of inflammatory mediators was determined by en- zyme-linked immunosorbent assay （ELISA）, such as prostaglandin E2 （PGE2）, tumor necrosis factor ot （TNF-ot） interleukin- 1 β （IL-1 β） and interleukin 6 （IL-6） in cell supernatant. The concentrations of inflammatory medi- 9 ators were calculated according to the standard curves generated by each of the recombinant cytokines provided with LPS can induce RAW264.7 cells to promote the pro- the ELISA kits. Results Compared with the control group, TNF-α, IL-1β and IL-6. Compared with the duction of inflammatory mediators （P 〈 0.01 ） , including NO, PGE2, model group, ethanol fraction significantly suppressed LPS induced release of inflammatory mediators such as nitric NO, PGE2, TNF-α, IL-1β and IL-6 in a good dose dependent manner （P 〈 0.05, P 〈 0.01 ）. Conclusions Eth- anol fraction could significantly inhibit the production of LPS-induced inflammatory response in RAW264.7 cells, and its anti-inflammatory effect may be related to reduce the production of inflammatory mediators NO, PGE2, TNF-α, IL-1 β and IL-6. These results demonstrate that the ethanol fraction is the bioactive component of Disporum can- toniense （Lour.） merr. , and the ethanol fraction will be further developed as a herbal remedy for preventive and/ or curative purposes in various inflammatory diseases.

关键词： Disporum cantoniense ( Lour. ) merr. LPS anti-inflammatory effects inflammatory mediators NO PGE2 TNF-α IL-6

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Responses of Japanese Soybeans to Hypoxic Condition at Rhizosphere Were Different Depending upon Cultivars and Ambient Temperatures

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American Journal of Plant Sciences 2013年第6期4卷 1297-1308页

作者： Yutaka Jitsuyama Research Faculty of Agriculture Hokkaido University Sapporo Japan.

To investigate the soybean (Glycine max merr.) wet endurance, and the affect of the maturity and the ambient temperature to the response, the plantlet in growth stage ranged from R1 to R2 of 8 Japanese soybean cultivars which characterized as various wet endurance in a past report were cultured under hypoxic-hydroponic condition for a month. Two experiments at different periods differed significantly in temperature of air and hydroponic solution, but the oxygenic condition were similar each experiments, as aimed for. And also, control and hypoxia treatments significantly differed in dissolved oxygenic concentration in each experiments. The hypoxic condition at higher temperature induced the reduction of survival of plantlet up to 70%. At higher temperature, the survival rate of late maturity types “Yuzuru” and “Shirotsurunoko” were decreased significantly in hypoxic condition than in control. Similarly, the shoot dry matters of them were also decreased significantly in hypoxia at higher temperature. The tendency of the decreasing in hypoxia was remarkable in the pod dry matter, and the symptoms were shown also at the lower temperature. From these results, soybean’s hypoxic tolerant may be reflected with the wet endurance, the process of hypoxic damages can be divided to sudden death symptom and biomass decreasing, and the mechanisms of hypoxic tolerance might be affected strongly by ambient temperature, and absolutely controlled with the genetic background.

关键词： Ambient Temperature Converted Field from Paddy Glycine max merr. Hydroponic Culture Hypoxia Low Oxygen Wet Endurance

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斑叶野木瓜容器播种育苗技术

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林业科技通讯 2017年第5期 29-30页

作者：游彩云梅拥军刘菁钟声祥江西环境工程职业学院江西赣州341000 江西赣州中学江西赣州341000 江西赣州客家风情园江西赣州341000

斑叶野木瓜(Stauntonia maculata merr.)是我国特有果木之一,2013年发现在赣州有分布。2014—2016年间于赣州市客家风情园基地开展斑叶野木瓜容器播种育苗试点,总结了育苗的基质配制、播种技术以及苗期光控管理等重要环节的技术。

关键词：斑叶野木瓜 Stauntonia maculata merr. 容器播种育苗光照赣州

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