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STUDY ON WAYS OF FORCING INTERCELLULAR CYTOPLASM AND CHROMATIN MIGRATION TO TRANSFER EXTERNAL GENETIC SUBSTANCES TO PLANT CELLS BY ARTIFICIAL MOTIVE FORCE

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Science China Chemistry 1992年第3期35卷 291-303页

作者：吴伯骥陈毅平崔亚亚郑国锠杨庆兰 Chengdu Institute of Biology Academia Sinica Chengdu 610015 PRC Cytology Lab of Lanzhou University Lanzhou 730000 PRC

A new cell engineering technique (L. B. technique) was established in our Lab. At first, the physical and chemical methods were used to facilitate the reestablishment of intercellular contacts and plasmodesma channels between different parents, thus forcing cytoplasm and chromatin to pass the cell wall with different qualities and quantities from one cell to others, through the enlarged intercellular plasmodesma channels or the vulnerable regions and the holes on the cell wall formed differently in growth and thickness in the process of cell wall formation to introduce external genetic substances or gene groups into plant cells. There are different ways, frequencies and strengths for the migration between the cells in different growth and development regions or the same growth and development region. In this paper we advance the mechanism of cytoplasm and chromatin migration through the cell wall: There are a large number of plasmodesma channels or vulnerable regions and holes different in growth and thickness during the formation of the cell walls between meristematic cells. Low osmotic pressure and low pH value K+ solution treatments force the augmentation of plasmic streaming, and the streaming of karyolymph, the relaxation of the cell wall, the formation of more holes on the cell wall and the enlargement of plasmodesma channels in meristematic cells. All these make a proper structural and physiological condition for migrating through cell walls. Osmotic pressure and centrifugal forces are the practical forces forcing cytoplasm and chromatin to migrate extensively and transfer external genetic substances or gene groups to plant cells.

关键词： meristematic cell low osmotic pressure and low pH value K+ solution L. B. technique intercellular plasmodesma channel.

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Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of <i>Kaempferia parviflora</i>

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American Journal of Plant Sciences 2014年第18期5卷 2693-2699页

作者： Ab Rahman Zuraida Othman Ayu Nazreena Kamarulzaman Fatin Liyana Izzati Ahmad Aziz Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Kuala Terengganu Malaysia

Callus and suspension cells culture of Kaempferia parviflora was successfully established. meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism. The medium containing combination of 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic acid (NAA) promoted the highest callus induction at 20%. Transferring the initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells revealed that the cells continued to grow until 50 days of culture and showed the highest peak (fresh weight) at 40 days in all different initial weight tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to produce the highest in weight when suspended in liquid medium supplemented with 1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.

关键词： Kaempferia parviflora Suspension Cultures meristematic Shoots Callus Induction

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