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Identification of SNPs and expression patterns of FZD3 gene and its effect on wool traits in Chinese merino sheep(Xinjiang Type)

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Journal of Integrative Agriculture 2019年第10期18卷 2351-2360页

作者： ZHAO Bing-ru FU Xue-feng TIAN Ke-chuan HUANG Xi-xia DI Jiang BAI Yan XU Xin-ming TIAN Yue-zhen WU Wei-wei ABLAT Sulayman ZENG Wei-dan HANIKEZI Tulafu College of Animal Science Xinjiang Agricultural UniversityUrumqi 830052P.R.China Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep Institute of Animal ScienceXinjiang Academy of Animal SciencesUrumqi 830011P.R.China

As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (Pmerino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).

关键词： Chinese merino sheep (Xinjiang Type) FZD3 single nucleotide polymorphism (SNP) expression pattern association analysis wool traits

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扫描式F-P干涉仪在merino观测中的热层风结果与分析

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地球物理学报 2014年第11期57卷 3688-3694页

作者：胡国元艾勇张燕革单欣顾健武汉大学电子信息学院武汉430070

merino是沿120°E/60°W子午圈的全球联网观测项目,热层中性风是其中一个十分重要的观测参数.本文介绍了武汉大学扫描式F-P干涉仪(SFPI)及其在merino第一轮观测中的结果,并与同站点的流星雷达在98km高度上的测风数据进行了验证,分析表... 详细信息

merino是沿120°E/60°W子午圈的全球联网观测项目,热层中性风是其中一个十分重要的观测参数.本文介绍了武汉大学扫描式F-P干涉仪(SFPI)及其在merino第一轮观测中的结果,并与同站点的流星雷达在98km高度上的测风数据进行了验证,分析表明两台设备的吻合性很好,SFPI风速测量的误差分别为8 m·s-1(557.7nm)和7.8m·s-1(630.0nm).本文对地基扫描式FPI的误差做了详细分析,结果表明在FPI仪器设计之初,就需要考虑标准具通光孔径D和成像物镜焦距f的合理组合,在目前CCD和标准具规格的限制下,增加f会提高测风精度,但会牺牲通光量,可通过设置CCD为Binning读出模式来提高灵敏度.在FPI进行风场观测时,要对设备工作的环境温度进行控制,特别是控制标准具的温度漂移.FPI的测风精度可以理论推导,但实际测风误差还与反演算法、元器件的加工和安装精度有关,国际通用的误差评价方法是用统计偏差来表示.

关键词： merino FPI 热层水平风误差分析

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Booroola merino羊Fec^B基因的研究及其在绵羊育种中的应用

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中国草食动物 2004年第6期24卷 44-46页

作者：李拥军薛慧文甘肃农业大学动物科技学院兰州730070 扬州大学畜牧兽医学院

FecB 基因是控制绵羊多胎性状的主效基因 ,对绵羊育种具有重要意义。文章就FecB 基因的遗传方式、分离定位以及生理效应等方面的研究现状作一阐述 ,并就FecB 基因在绵羊育种中的应用效果及前景展望进行了探讨。

关键词： Booroola merino 羊 Fec^B基因绵羊育种多胎性状遗传生理效应

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Adaptability and Application of Australian merino Sheep in the Alpine Pastoral Areas

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Animal Husbandry and Feed Science 2017年第5期9卷 279-281页

作者： Wen Yazhou Li Fanwen Wang Tianxiang Liu Jigang Li Wenhui Li Guiying Gansu Provincial Sheep Breeding Technology Extension Station Zhangye 734031 China

[Objective] The paper was to study the adaptability of Australian merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian merino sheep for hybridizing with Gansu alpine fine wool sheep,the improvement effect of offspring was observed. [Result]The introduced Australian merino sheep had good adaptability in the alpine pastoral areas with good hair quality,strong cold resistance and disease resistance,and they could grow and reproduce healthily in the alpine pastoral areas. The application effect of the ram was outstanding,showing large breeding amount,high conception rate and survival rate.[Conclusion]The improvement effect of offspring of Australian merino sheep and Gansu alpine fine wool sheep was significant,and the growth performance was improved greatly.

关键词： Australian merino sheep Alpine pastoral area Adaptability Application Research

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I LOVE merino——2010年美丽诺家族品牌年度庆典

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流行色 2011年第1期 149-149页

2010年12月17日晚,在上海中心城区规模最大的秀场——静安800show,由澳大利亚羊毛发展公司(AWI,Australian Wool Innovation Limited)携手旗下七大品牌(女装品牌EP雅莹、ICICLE之禾和JNBY江南布衣,男装品牌CROQUIS速写,户外品牌KROCEUS... 详细信息

2010年12月17日晚,在上海中心城区规模最大的秀场——静安800show,由澳大利亚羊毛发展公司(AWI,Australian Wool Innovation Limited)携手旗下七大品牌(女装品牌EP雅莹、ICICLE之禾和JNBY江南布衣,男装品牌CROQUIS速写,户外品牌KROCEUS科诺修斯,婴童品牌les enphants丽婴房,内衣品牌SCHIESSER舒雅)举行了一场以"I LOVE merino"为主题的2010年美丽诺家族品牌年度庆典。

关键词： I LOVE merino 舒雅 Australian 纯羊毛标志市场推广运动产品供应链合作伙

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Evaluation of “Indigenous Vaccine” Developed Using “Indian Bison Type” Genotype of <i>Mycobacterium avium subspecies paratuberculosis</i>Strain “S5” of Goat Origin in a Sheep Flock Endemic for Johne’s Disease: A Three Years Trial in India

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World Journal of Vaccines 2013年第2期3卷 52-59页

作者： Shoor Vir Singh Ajay Vir Singh Pravin Kumar Singh Saurabh Gupta Harendra Singh Brajesh Singh Omalur Rajendran Vinodh Kumar Aripudur Sellappan Rajendiran Narendra Nath Swain Jagdip Singh Sohal Central Institute for Research on Goats Mathura Indi Central Institute for Research on Goats Mathura India Southern Regional Research Centre Central Sheep and Wool Research Institute Kodaikanal India

“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) of Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep flock of Bharat merino breed located in Mannavanur town of Tamil Nadu in South India. Therapeutic efficacy of the vaccine was evaluated for 3 years between 2008 and 2010, on the basis of improvements in productivity (body weights, reproductive efficiency and survivability), physical condition, clinical symptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) and infection load in feces (shedding). After immunization of the flock in 2008, the successive progenies of 112 and 53 lambs born in 2009 and 2010, respectively were vaccinated. Whereas, 40 lambs born to control animals were kept as unvaccinated controls. Though gain in body weights in vaccinated versus controls were not significant in 2008, growth rates were distinctly superior in Ist and IInd generations of vaccinated lambs. Reproductive performance (tupping percent) and survivability of lambs and adult sheep improved significantly. There was overall reduction in yearly morbidity (diarrhea) and mortality rates of the flock in post vaccination years. Shedding of MAP in feces was reduced in vaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the control sheep. Seromonitoring of the animals by “indigenous ELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding and improved immunity and productivity of Bharat merino flock, endemic for Johne’s disease.

关键词： Paratuberculosis Indigenous Vaccine Indian Bison Type Bharat merino Sheep India

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