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Preparation and Immunogenicity of Serogroup B meningococcal OS-OMPC Conjugates

Biomedical and Environmental Sciences 1999年第4期12卷 296-303页

作者： SUNYIN－YAN HUXU－JING InstituteofEpidemiologyandMicrobiology ChineseAcademyofPreventiveMedicineBeijing102206 InstituteofEp

The objective of the present study is to purify oligosaccharides (OSs) from serogroup B meningococci (3407 or 542852) with common lipooligosaccharide (LOS) immunotypes prevalent in China and to successtully conjugate them to their outer membrane protein complexes (OMPCs).Conjugates possessing broad cross-reactivity with different serogroup B meningococci were *** LOSs from the above two strains were purified on a Sephghex G75 column and hydrolyzed with acetic acid, and then the pare OSs were obtained by using a Sephadex G50 chromatographic column. The pure OSs were conjugated to corresponding OMPCs by carbodiimide mediated condensation. Mice were immunized with the OS-OMPC conjugates to produce antibodies. Immunognicities of the conjugates were tested by EUSA, bactericidal test and Western-blotting. The titers of antibodies against OS and LOS elicited by the two OS-OMPC conjugates increased respectively by 2. 5and 2 to 10 times as compared with those of antibodies against unconjugated OS and LOS. The sera elicited by the conjugates possessed a stronger bactericidal activity to the stndus (3407, 542852,29021) with the same LOS immunotype than to the strain with different LOS immunotype (86155).The results determined by ELISA indicated that the sera against the conjugaes showed strong response to the strains with the same or different LOS immunotypes. It was confirmed by westem-blotting analysis that the sera elicited by the two conjugates reacted with 42, 39 and 26 kDa proteins from the OMPC. Among the reactive bands, the 42kDa proteins were class 1 OMPs. The two serogroup B meningococcal OMPCs were strong immunogens and good carriers coupling with OSs as well. The immunogencity of the conjugaed OS was increased and the OS-OMPC conjugates of the serogroup B meogingococci showed a broad antigenic response to the strains tested.

关键词： Bacterial Outer Membrane Proteins Animals Antibodies Bacterial Blotting Western Enzyme-Linked Immunosorbent Assay Immunoconjugates Meningitis meningococcal Mice Neisseria meningitidis Oligosaccharides Research Support Non-U.S. Gov't

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Mandatory meningococcal vaccine, and other recommended immunisations: Uptake, barriers, and facilitators among health care workers and trainees at Hajj

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World Journal of Clinical Cases 2018年第16期6卷 1128-1135页

作者： Al-Mamoon Badahdah Mohammad Alfelali Amani S Alqahtani Saeed Alsharif Osamah Barasheed Harunor Rashid the Hajj Research Team Department of Family and Community Medicine Faculty of Medicine in RabighKing Abdulaziz UniversityJeddah 22252Saudi Arabia National Centre for Immunisation Research and Surveillance the Children’s Hospital at WestmeadWestmeadNSW 2145Australia the Discipline of Child and Adolescent Health the Faculty of Medicine and Healththe University of SydneyWestmeadNSW 2145Australia Saudi Food and Drug Authority Riyadh 13312Saudi Arabia Command and Control Centre of Infectious Diseases of Public Health Department of Ministry of Health Taif 26521Saudi Arabia the Executive Administration of Research and Innovation at King Abdullah Medical City in Holy Capital Makkah 24246Saudi Arabia Marie Bashir Institute for Infectious Diseases and Biosecurity School of Biological Sciences and Sydney Medical SchoolUniversity of SydneyWestmeadNSW 2145Australia 不详

AIM To evaluate the uptake of a mandatory meningococcal, a highly recommended influenza, and an optional pneumococcal vaccine, and to explore the key factors affecting vaccination rate among health care workers(HCWs) during the *** An anonymous cross-sectional online survey was distributed among HCWs and trainees who worked or volunteered at the Hajj 2015-2017 through their line managers, or by visiting their hospitals and healthcare centres in Makkah and Mina. Overseas HCWs who accompanied the pilgrims or those who work in foreign Hajj medical missions were excluded. Pearson's χ2 test was used to compare categorical variables and odds ratio(OR) was calculated by "risk estimate" statistics along with 95% confidence interval(95%CI).RESULTS A total of 138 respondents aged 20 to 59(median 25.6) years with a male to female ratio of 2.5:1 participated in the survey. Only 11.6%(16/138) participants reported receiving all three vaccines, 15.2%(21/138) did not receive any vaccine, 76.1%(105/138) received meningococcal, 68.1%(94/138) influenza and 13.8%(19/138) pneumococcal vaccine. Females were more likely to receive a vaccine than males(OR 3.6, 95%CI: 1.0-12.7, P < 0.05). Willingness to follow health authority's recommendation was the main reason for receipt of vaccine(78.8%) while believing that they were up-to-date with vaccination(39.8%) was the prime reason for non-receipt. CONCLUSION Some HCWs at Hajj miss out the compulsory and highly recommended vaccines; lack of awareness is a key barrier and authority's advice is an important motivator. Health education followed by stringent measures may be required to improve their vaccination rate.

关键词： Influenza meningococcal disease Hajj Health care workers Pneumococcal disease Vaccine uptake

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Proteoliposome and Polysaccharide-Based meningococcal Vaccine Are Immunogenic in Infants and Toddlers and Primes for Memory against Serogroup C Polysaccharide

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World Journal of Vaccines 2013年第2期3卷 77-87页

作者： Oliver Pérez Belkis Romeu Judith del Campo Caridad Zayas Miriam Lastre Immunology Department Basic Research Vice Direction Finlay Institute Havana Cuba

Neisseria meningitidis capsular polysaccharides are the main target of the protective immune response against bacterial meningitis. They are thymus-independent type 2 (TI-2) antigens that are poorly immunogenic and not protective in young children, and their administration may impair subsequent challenge with the same polysaccharide. These problems have been addressed using three different vaccines consisting of 1) polysaccharide alone, 2) polysaccharide covalently conjugated to a carrier protein, and 3) polysaccharide with Proteoliposome (PL) adsorbed onto Al(OH)3. VA-MENGOC-BC? is one of the third types of vaccine. It contains PL (detergent-extracted external membrane proteins forming vesicles) and polysaccharide (Ps) from N. meningitidis serogroups B and C (PsC), respectively. Nevertheless, there is a concern that to overcome the TI-2 nature of Ps the covalently conjugation to a carrier is mandatory. Therefore, we evaluated the immune response induced by VA-MENGOC-BC? in infants and toddlers in order to determine whether it stimulates the response against the PsC. High IgG anti PsC and anti PL responses were seen following the administration of two doses in infants and toddlers, after a 3rd dose in pre-teenagers, and after confirmed carrier stages in young adults. The anti PL IgG response persisted longer than anti PsC IgG response and IgM response against both antigens was maintained. An IgG1 anti PL response predominated, as well as IgG4 > IgG3 > IgG1 anti PsC responses. These results suggest that non-covalent incorporation of PsC onto Al(OH)3 containing?PL as adjuvant is immunogenic, primes for memory, and induces long-lasting specific antibody response. The improved PsC immunogenicity of this vaccine may be due to the preferential and potent Th1 response induced in mice and human by the PL as adjuvant.

关键词： Proteoliposome meningococcal Vaccine Infants Memory Response Polysaccharide

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Theoretical assessment of the impact of desert aerosols on the dynamical transmission of meningitidis serogroup A

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International Journal of Biomathematics 2019年第5期12卷 263-299页

作者： A. Otimar Bah M. Lam A. Rah S. Bowong Department of Mathematics Faculty of Science and Technic Universie Cheikh Anta Diopde DakarDakar-FannSenegal Department of Computer Engineering National Advanced School of Engineering Universite Cheikh Anta Diopde Dakar Dakar-FannSenegal Laboratory of Mathematics Department of Mathematics and Computer ScienceFaculty of ScienceUniversity of Douala P.O.Box 24157DoualaCameroon UMI 209 1RD and UPMC UMMISCO 32 Avenue Henri Varagnat 93143 Bondy CedexFrance Project Team GRIMCAPE University of Yaounde YaoundeCameroon The African Center of Excellence in Information and Communication Technologies(CETIC)National Advanced School of Engineering University of Yaounde 1 YaoundeCameroon

This paper has been motivated by the following biological question: how influential me desert uerosols in the transmission of meningitidis serogroup A (MenA)? A mathematical model for the dynamical transmission of MenA is considered,with the aim of investigating the impact of desert *** analysis of the model has been performed in order to determine the impact of related parameters on meningitis *** derive the basic reproduction number *** prove that there exists a threshold parameter ζ such that when R0 <ζ< 1,the disease-free equilibrium is globally asymptotically stable (GAS).However,when ζ< R0 < 1,the model exhibits the phenomenon of backward *** the endemic level,we show that the number of infectious individuals in the presence of desert aerosols is larger than the correspond-ing number without the presence of desert *** conjunction with the inequality R0^0< R0 where R0^0 is the basic reproduction number without desert aerosols,we found that the ingestion of aerosols by carriers will increase the endemic level,and the severity of the *** suggests that the control of MenA passes through a combination of a large coverage vaccination of young susceptible individuals and the production of a vaccine with a high level of efficacy as well as respecting the hygienic rules to avoid the inhalation of desert *** results are supported by numerical simulations.

关键词： Nonlinear dynamical systems epidemiological models serogroup A meningococcal infection stability bifurcation

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Vaccinations against respiratory infections in Arabian Gulf countries: Barriers and motivators

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World Journal of Clinical Cases 2017年第6期5卷 212-221页

作者： Amani S Alqahtani Daniah M Bondagji Abdullah A Alshehari Mada H Basyouni Tariq M Alhawassi Nasser F Bin Dhim Harunor Rashid School of Public Health the University of Sydney National Centre for Immunisation Research and Surveillance of Vaccine Preventable Diseases (NCIRS) the Children’s Hospital at Westmead and the Discipline of Paediatrics and Child Health Sydney Medical School the University of Sydney Ministry of Health Investigational Drugs and Research Unit King Khalid University Hospital College of Pharmacy King Saud University Medication Safety Research Chair College of Pharmacy King Saud University Saudi Food and Drug Authority

AIM To study the uptake, barriers and motivators of influen-za, pneumococcal, meningococcal and pertussis vaccines among members of public in Arabian Gulf *** A cross-sectional survey among the Gulf Cooperation Council(GCC) countries' residents. Data collected electronically through a smartphone app. The survey variables aimed to investigate the respondents' awareness about vaccines against influenza, pneumococcal, meningococcal and pertussis infections. Collected data concerning the respondents' socio-demographic characteristics, their perception toward vaccine uptake and the factors that motivate or demotivate them from taking influenza vaccine. The data were analysed statistically using the SPSS v.23.0. Differences in the characteristics of users from different countries were quantified through bivariate analysis. Other important variables and controlling factors were studied using logistic regression. RESULTS A total of 1812 respondents participated in the study. Their mean age was 27 years, 82% were male and 24% had ≥ 1 chronic diseases. The overall uptake of influenza vaccine was 17%(21% among "at risk" people) and ranged from 15% in Saudi Arabia to 24% in Qatar. Doctor's advice(23%) and a perception of having low body immunity(21%) were the main cited reasons for being vaccinated, whereas unawareness about the vaccine(43%) was the main barrier. The overall uptake of pneumococcal vaccine in the preceding three years was 22%(25% among "at risk" individuals) and ranged from 0% in Bahrain to 79% in Kuwait. The overall uptake of pertussis vaccine was 16%(31% among "vulnerable" people), and ranged from 7% in Saudi Arabia to 75% in Oman. The overall uptake of meningococcal vaccine was 20%(29% among the "at risk" people) and ranged from 3% in Oman to 50% in *** The vaccination uptake across GCC countries is suboptimal and varies widely across the countries. Further research is needed to unearth the reasons and formulate action plan.

关键词： Gulf Cooperation Council Influenza meningococcal vaccine Motivators and barriers Pertussis vaccine Pneumococcal vaccine Respiratory infections

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