检索结果-南通市图书馆

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

引用

Nano Research 2015年第3期8卷 774-789页

作者： Mohammed Jamshad Vinciane Grimard Ilaria Idini Tim J. Knowles Miriam R. Dowle Naomi Schofield Pooja Sridhart Yupin Lin Rachael Finka Mark Wheatley Owen R. T. Thomas Richard E. Palmerr Michael Overduin Cedric Govaerts Jean-Marie Ruysschaert Karen J. Edler Tim R. Dafforn School of Biosciences University of Birmingham Edgbaston Birmingham B 15 2TT UK Universite Libre de Bruxelles SFMB - CP206/2 Bd. du Triomphe Acces 2 1050 Bruxelles Belgium Department of Chemistry University of Bath Claverton Down Bath BA2 7AY UK School of Cancer Studies University of Birmingham Edgbaston Birmingham B 15 2 TT UK Nanoscale Physics Research Laboratory and PSIBS School of Physics and Astronomy University of Birmingham Edgbaston Birmingham B 15 2TT UK School of Chemical Engineering University of Birmingham Edgbaston Birmingham B 15 2TT UK

In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer （poly（styrene-co-maleic add）, SMA） to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle （SMALP） using data from small angle neutron scattering （SANS）, electron microscopy （EM）, attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, differential scanning calorimetry （DSC） and nuclear magnetic resonance spectroscopy （NMR）. We show that the particle is disc shaped containing a polymer ＂bracelet＂ encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.

关键词： nanoparticles lipid polymer membrane proteins structure detergent

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Erythrocyte membrane proteins and membrane skeleton

引用

Frontiers in Biology 2007年第3期2卷 247-255页

作者： LU Yiqin LIU Junfan Department of Biochemistry Xiangya School of MedicineCentral South UniversityChangsha 410078China

Considerable advances in the research field of erythrocyte membrane were achieved in the recent two *** findings in the structure-function correlation and interactions of erythrocyte membrane proteins have attracted extensive *** progress was also made in the molecular pathogenesis of erythrocyte membrane *** in the composition,function and interac-tion of erythrocyte membrane proteins,erythrocyte mem-brane skeleton,and relevant diseases are briefly described and summarized here on the basis of domestic and world literatures.

关键词： erythrocyte membrane proteins interaction functional disorders membrane skeleton

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Artificial Nucleic Acid Tractor-Directed Simultaneous Depletion of Oncogenic membrane proteins Without Hijacking Proteolysis-Specific Actuator

引用

CCS Chemistry 2024年第2期6卷 439-449页

作者： Zhen Zou Songlan Pan Qian Xue Ting Chen Ziyun Huang Bei Qing Pengfei Liu Conghui Zhao Yunlin Sun Erhu Xiong Ronghua Yang Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of EducationCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081 School of Chemistry and Chemical Engineering Hunan Provincial Key Laboratory of CytochemistryChangsha University of Science and TechnologyChangsha 410114 The Second Xiangya Hospital Central South UniversityChangsha 410011

Targeted protein degradation(TPD)is an emerging tool for degrading proteins of interest,which affords an attractive modality for cancer ***,the present TPD technologies must engage a proteolysis-specific actuator to initiate degradation of targeted proteins in the proteasome or ***,we report an artificial tractor that can induce endocytosis-mediated protein depletion without hijacking a proteolysis-specific *** this design,bispecific aptamer chimeras(BSACs)are established,which can bridge human epidermal growth factor receptor 2(ErbB-2),an important biomarker in a common important biomarker in cancer,with membrane proteins of *** advantage of the property of aptamer-induced endocytosis and digestion of ErbB-2,another membrane protein is translocated into the lysosome in a hitchhike-like manner,resulting in lysosomal proteolysis along with *** strategy frees the TPD from the fundamental limitation of proteolysis-specific actuator and allows simultaneous regulation of the quantity and function of two oncogenic receptors in a cell-type-specific manner,expanding the application scope of TPD-based therapeutics.

关键词： targeted protein degradation aptamer epidermal growth factor receptor 2 lysosome membrane proteins cancer therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Structure Prediction of membrane proteins

引用

Genomics, Proteomics & Bioinformatics 2004年第1期2卷 1-5页

作者： ChunlongZhou YaoZheng YanZhou HangzhouGenomicsInstitute／JamesD.WatsonInstituteofGenomeSciences ZhejiangUniversity／KeyLaboratoryofBioinformaticsofZhejiangProvinceHangzhou310008China CenterforEngineeringandScientificComputation ZhejiangUniversityHangzhou310027China

There is a large gap between the number of membrane protein (MP) sequencesand that of their decoded 3D structures, especially high-resolution structures, due to difficultiesin crystal preparation of MPs. However, detailed knowledge of the 3D structure is required for thefundamental understanding of the function of an MP and the interactions between the protein and itsinhibitors or activators. In this paper, some computational approaches that have been used topredict MP structures are discussed and compared.

关键词： structure prediction membrane proteins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Activation of nuclear factor κB and induction of inducible nitric oxide synthase by lipid-associated membrane proteins isolated from Mycoplasma penetrans

引用

Chinese Medical Journal 2004年第7期117卷 997-1001页

作者：曾焱华吴移谋张文波余敏君朱翠明谭立志 Department of Microbiology

Background This study was designed to investigate the potential pathogenicity of Mycoplasma penetrans (M. penetrans) and its molecular mechanisms responsible for the induction of iNOS gene expression in mouse macrophages stimulated by lipid-associated membrane proteins (LAMPs) prepared from M. *** Mouse macrophages were stimulated with M. penetrans LAMPs to assay the production of nitric oxide (NO). The expression of inducible nitric oxide synthase (iNOS) was detected by RT-PCR and Western blotting. The activity of nuclear factor κB (NF-κB) and the effects of pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB, on the production of nitric oxide and the expression of iNOS were also assessed in mouse macrophages treated with M. penetrans LAMPs by indirect immunofluorescence and Western *** M. penetrans LAMPs stimulated mouse macrophages to produce nitric oxide in a dose- and time-dependent manner. The mRNA and protein levels of iNOS were also upregulated in response to LAMP stimulation and inhibited by PDTC treatment. M. penetrans LAMPs were found to trigger NF-κB activation, a possible mechanism for the induction of iNOS *** This study demonstrated that M. penetrans may be an important etiological factor of certain diseases due to the ability of M. penetrans LAMPs to stimulate the expression of iNOS, which is probably mediated through the activation of NF-κB.

关键词： mycoplasma penetrans nuclear factor kappa B nitric oxide synthase membrane proteins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T

引用

World Journal of Gastroenterology 2005年第43期11卷 6880-6883页

作者： Tian-Yi Ying Jun-Jun Wang Heng-Liang Wang Er-Ling Feng Kai-Hua Wei Liu-Yu Huang Pei-Tang Huang Cui-Fen Huang Beijing Institute of Biotechnology State Key Lab of Pathogen and Biosecurity Beijing 100071 China Beijing Institute of Pharmaceutical Chemistry Beijing 102205 China

AIM： To screen the immunogenic membrane proteins of Shigella Aexneri 2a 2457T. METHODS： The routine two-dimensional polyacrylamide gel electrophoresis （2-DE） and Western blotting were combined to screen immunogenic proteins of S. Aexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry （MALDI-TOF-MS） was performed to determine the molecular weight of peptides. Electrospray ionization （ESI-MS/MS） was performed to determine the sequences of the interesting peptides. RESULTS： A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein （YaeT） was detected, which might be a candidate target of vaccine. CONCLUSION： membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum.

关键词： Shigella flexneri 2a 2457T Immunoproteomics membrane proteins

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical modification of membrane proteins

Chemical modification of Membrane Proteins

引用

第十届全国化学生物学学术会议

作者： Ji-Shen Zheng School of Life Sciences University of Science and Technology of China

proteins with chemical modifications can re-design their structures and functions to impart immense chemical, physical, and biological diversity. membrane proteins play critical roles in many physiological processes, such as ion transportation and signal transduction which can be modulated by post-translational modifications. Chemical synthesis of membrane proteins represents a unique method to provide site-specific modified protein probes for their functional actions. However, the hydrophobic nature of membrane proteins causes severe handling troubles during the transmembrane peptide ligation and purification steps. In our studies, we describe the removable backbone modification（RBM） methods to modulate the solubilizing property of transmembrane peptides/membrane proteins. The membrane protein segments with the RBM groups behave almost like the ordinary water-soluble peptides to facilitate the ligation, purification, and characterizations. Its practicality and usefulness are demonstrated by the successful preparation of chemically modified membrane proteins, such as the phosphorylated M2 channel form influenza A virus and site-specifically isotope-labeling p7 cation-specific ion channel from hepatitis C virus.

关键词： Chemical modification of proteins Removable backbone modification membrane proteins site-specific labeling

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Three Monoclonal Antibodies of membrane proteins for Detecting Citrus HLB

Three Monoclonal Antibodies of Membrane Proteins for Detecti...

引用

中国植物病理学会2019年学术年会

作者： Wu Xiaoyan Integrative Microbiology Research Centre South China Agricultural University

Huanglongbing（HLB）,caused by the pathogen Candidatus Liberibacter asiaticus（CLas）,is one of the most destructive disease of *** by the Asian citrus psyllid,CLas is the phloem-residing bacteria and could not be cultured under in vitro *** integrated control methods for HLB depend on early detection and eradication of HLB-infected citrus *** identify infected citrus and to interdict the spread of disease timely are the key points to limit ***,methods for detecting HLB include electron microscopy,PCR,and qPCR,but these conventional detection methods are time consuming,expensive,and inconvenient for *** an aim to develop a convenient and rapid hands-on machine to detect HLB,we set to generate HLB-specific monoclonal *** bioinformatics analysis,we selected three genes encoding outer membrane proteins as potential *** proteins encoded by these genes were purified by affinity chromatography,and designated as Q1 G,Q2 G and Q3 G,*** anti-Q1 G,anti-Q2 G and antiQ3 G monoclonal antibodies will be prepared soon.

关键词： huanglongbing membrane proteins monoclonal antibody

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Cellular and organellar membrane-associated proteins in haloarchaea: Perspectives on the physiological significance and biotechnological applications

引用

Science China(Life Sciences) 2012年第5期55卷 404-414页

作者： CAI Lei ZHAO DaHe HOU Jing WU JinHua CAI ShuangFeng DASSARMA Priya XIANG Hua State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China Department of Microbiology ＆ Immunology University of Maryland School of Medicine Baltimore MD 21202 USA

Halophilic archaea (haloarchaea) inhabit hypersaline environments,tolerating extreme salinity,low oxygen and nutrient availability,and in some cases,high pH (soda lakes) and irradiation (saltern ponds).membrane-associated proteins of haloarchaea,such as surface layer (S-layer) proteins,transporters,retinal proteins,and internal organellar membrane proteins including intracellular gas vesicle proteins and those associated with polyhydroxyalkanoate (PHA) granules,contribute greatly to their environmental *** review focuses on these haloarchaeal cellular and organellar membrane-associated proteins,and provides insight into their physiological significance and biotechnological potential.

关键词： halophilic archaea membrane proteins physiology biotechnology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An improved installation of 2-hydroxy-4-methoxybenzyl(iHmb)method for chemical protein synthesis

引用

Chinese Chemical Letters 2024年第5期35卷 145-150页

作者： Ying Li Long-Jie Wang Yong-Kang Zhou Jun Liang Bin Xiao Ji-Shen Zheng The First Affiliated Hospital of USTC MOE Key Laboratory for Membraneless Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Chemistry University of Science and Technology of ChinaHefei 230027China

The 2-hydroxy-4-methoxybenzyl(Hmb)backbone modification can prevent amide bond-mediated sidereactions(e.g.,aspartimide formation,peptide aggregation)by installing the removable Hmb group into a peptide bond,thus improving the synthesis of long and challenging peptides and ***,its use is largely precluded by the limited Hmb’s installation *** this report,an improved installation of Hmb(iHmb)method was developed to achieve the flexible installation and the convenient removal of *** iHmb method involves two critical steps:(1)oxidative diazotization of the readily installed 2-hydroxy-4-methoxy-5-amino-benzyl(Hmab)to give 2-hydroxy-4-methoxy-5-diazonium-benzyl(Hmdab)by combining soamyl nitrite(IAN)/HBF_(4),and(2)reductive elimination of Hmdab to give the desired Hmb by 1,2-ethanedithiol(EDT).The iHmb method enables the installation of Hmb at any primary amino acid including the highly sterically hindered amino acids(e.g.,valine and isoleucine).The practicality and utility of the iHmb method was demonstrated by one-shot solid-phase synthesis of a challenging aspartimide-prone peptide,the mirror-image version of a hydrophobic peptide and a long-chain peptide up to ***,the iHmb method can be utilized to facilitate chemical protein ligation,as exemplified by the synthesis of the single-spanning membrane protein *** iHmb method expands the toolkit for peptide synthesis and ligation and facilitates the preparation of peptides/proteins.

关键词： Chemical protein synthesis Solid-phase peptide synthesis Removable backbone modification 2-Hydroxy-4-methoxybenzyl(Hmb)group Aspartimide-prone peptides Difficult peptides membrane proteins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：