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Assessing the response of human primary macrophages to defined fibrous architectures fabricated by melt electrowriting

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Bioactive Materials 2023年第3期21卷 209-222页

作者： Carlotta Mondadori Amit Chandrakar Silvia Lopa Paul Wieringa Giuseppe Tal`o Silvia Perego Giovanni Lombardi Alessandra Colombini Matteo Moretti Lorenzo Moroni IRCCS Istituto Ortopedico Galeazzi Cell and Tissue Engineering LaboratoryMilanItaly Complex Tissue Regeneration Department MERLN Institute for Technology-Inspired Regenerative MedicineMaastrichtthe Netherlands IRCCS Istituto Ortopedico Galeazzi Laboratory of Experimental Biochemistry and Molecular BiologyMilanItaly Department of Athletics Strength and ConditioningPozna´n University of Physical EducationPozna´nPoland IRCCS Istituto Ortopedico Galeazzi Orthopaedic Biotechnology LabMilanItaly Regenerative Medicine Technologies Lab Ente Ospedaliero CantonaleBellinzonaSwitzerland Service of Orthopaedics and Traumatology Department of SurgeryEnte Ospedaliero CantonaleLuganoSwitzerland Euler Institute Faculty of Biomedical SciencesUniversit`a della Svizzera ItalianaLuganoSwitzerland

The dual role of macrophages in the healing process depends on macrophage ability to polarize into phenotypes that can propagate inflammation or exert anti-inflammatory and tissue-remodeling *** scaf-fold geometry has been proposed as a strategy to influence macrophage behavior and favor the positive host response to ***,we fabricated Polycaprolactone(PCL)scaffolds by melt electrowriting(MEW)to investigate the ability of scaffold architecture to modulate macrophage *** human macrophages unpolarized(M0)or polarized into M1,M2a,and M2c phenotypes were cultured on PCL films and MEW scaffolds with pore geometries(square,triangle,and rhombus grid)characterized by different angles.M0,M2a,and M2c macrophages wrapped along the fibers,while M1 macrophages formed clusters with rounded *** bridges were formed only for angles up to 90◦.No relevant differences were found among PCL films and 3D scaffolds in terms of surface ***206 and CD163 were highly expressed by M2a and M2c macrophages,with M2a macrophages presenting also high levels of CD86.M1 macrophages expressed moderate levels of all *** rhombus architecture promoted an increased release by M2a macrophages of IL10,IL13,and sCD163 compared to PCL *** proangiogenic factor IL18 was also upregulated by the rhombus configuration in M0 and M2a macrophages compared to PCL *** interesting findings obtained for the rhombus architecture represent a starting point for the design of scaffolds able to modulate macrophage phenotype,prompting investigations addressed to verify their ability to facilitate the healing process in vivo.

关键词： Macrophage melt electrowriting Additive manufacturing Scaffold design Scaffold geometry

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3D printing of bio-instructive materials:Toward directing the cell

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Bioactive Materials 2023年第1期19卷 292-327页

作者： Piotr Stanisław Zielinski Pavan Kumar Reddy Gudeti Timo Rikmanspoel Małgorzata Katarzyna Włodarczyk-Biegun Polymer Science Zernike Institute for Advanced MaterialsUniversity of GroningenNijenborgh 49747 AGGroningenthe Netherlands Biotechnology Centre The Silesian University of TechnologyB.Krzywoustego 844-100GliwicePoland

Fabrication of functional scaffolds for tissue engineering and regenerative medicine applications requires material systems with precise control over cellular performance.3D printing is a powerful technique to create highly complex and multicomponent structures with well-defined architecture and *** this review paper,we explore extrusion-based 3D printing methods(EBP,i.e.,Near Field Electrospinning(NFES),melt electrowriting(MEW),Fused Deposition Modeling(FDM),and extrusion bioprinting)in terms of their ability to produce scaffolds with bio-instructive *** material systems provide spatio-temporal guidance for cells,allowing controlled tissue regeneration and *** physical and biochemical cues introduced to the EBP scaffolds are evaluated in their ability to direct cell alignment,proliferation,differentiation,specific ECM production,and tissue *** indicate that the cues have different impacts depending on the material system,cell type used,or coexistence of multiple ***,they must be carefully chosen based on the targeted *** propose future directions in bio-instructive materials development,including such concepts as metamaterials,hybrid living materials,and 4D *** review gathers the knowledge essential for designing new materials with a controlled cellular response,fabrication of advanced engineered tissue,and developing a better understanding of cell biology,especially in response to the biomaterial.

关键词： Tissue engineering Additive manufacturing melt electrowriting 3D scaffold Cell differentiation Biomaterials

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Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues

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Bioactive Materials 2023年第1期19卷 268-281页

作者： Arwa Daghrery Jessica A.Ferreira Jinping Xu Nasim Golafshan Darnell Kaigler Sarit B.Bhaduri Jos Malda Miguel Castilho Marco C.Bottino Department of Cariology Restorative Sciencesand EndodonticsSchool of DentistryUniversity of MichiganAnn ArborMIUnited States Department of Restorative Dental Sciences School of DentistryJazan UniversityJazanKingdom of Saudi Arabia Department of Orthopedics University Medical Center UtrechtUtrechtthe Netherlands Regenerative Medicine Center Utrechtthe Netherlands Department of Periodontics and Oral Medicine School of DentistryUniversity of MichiganAnn ArborMIUnited States Department of Mechanical Industrial and Manufacturing EngineeringUniversity of ToledoToledoOHUnited States EEC Division Directorate of EngineeringThe National Science FoundationAlexandriaVAUnited States Department of Clinical Sciences Faculty of Veterinary MedicineUtrecht UniversityUtrechtthe Netherlands Department of Biomedical Engineering Eindhoven University of TechnologyEindhoventhe Netherlands Institute for Complex Molecular Systems Eindhoven University of TechnologyP.O.Box 5135600 MB Eindhoventhe Netherlands Department of Biomedical Engineering College of EngineeringUniversity of MichiganAnn ArborMIUnited States

Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting *** limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the local immune ***,three-dimensional melt electrowritten(i.e.,poly(ε-caprolactone))scaffolds with tissue-specific attributes were engineered to guide differentiation of human-derived periodontal ligament stem cells(hPDLSCs)and mediate macrophage *** investigated tissue-specific scaffold attributes comprised fiber morphology(aligned ***)and highly-ordered architectures with distinct strand spacings(small 250μm and large 500μm).Macrophages exhibited an elongated morphology in aligned and highly-ordered scaffolds,while maintaining their round-shape on randomly-oriented fibrous *** of periostin and IL-10 were more pronounced on the aligned and highly-ordered *** hPDLSCs on the scaffolds with 500μm strand spacing show higher expression of osteogenic marker(Runx2)over 21 days,cells on randomly-oriented fibrous scaffolds showed upregulation of M1 *** an orthotopic mandibular fenestration defect model,findings revealed that the tissue-specific scaffolds(i.e.,aligned fibers for periodontal ligament and highly-ordered 500μm strand spacing fluorinated calcium phosphate[F/CaP]-coated fibers for bone)could enhance the mimicking of regeneration of natural periodontal tissues.

关键词： Periodontitis melt electrowriting 3D printing Inflammation Periodontal regeneration Scaffold

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Machine learning-enabled optimization of melt electro-writing three-dimensional printing

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Aggregate 2024年第3期5卷 258-267页

作者： Ahmed Choukri Abdullah Olgac Ozarslan Sara Soltanabadi Farshi Sajjad Rahmani Dabbagh Savas Tasoglu Department of Mechanical Engineering KoçUniversitySariyerTurkey Department of Biomedical Sciences and Engineering KoçUniversitySariyerTurkey KoçUniversity Arçelik Research Center for Creative Industries(KUAR) KoçUniversitySariyerTurkey Koc University Is Bank Artificial Intelligence Lab(KUIS AILab) KoçUniversitySariyerTurkey KoçUniversity Translational Medicine Research Center(KUTTAM) KoçUniversitySariyerTurkey Bogaziçi Institute of Biomedical Engineering Bogaziçi UniversityCengelkoyTurkey

melt electrowriting(MEW)is a solvent-free(i.e.,no volatile chemicals),a high-resolution three-dimensional(3D)printing method that enables the fabrication of semi-flexible structures with rigid *** its advantages,the MEW pro-cess is sensitive to changes in printing parameters(e.g.,voltage,printing pressure,and temperature),which can causefluid column breakage,jet lag,and/orfiber pulsing,ultimately deteriorating the resolution and printing *** spite of the commonly used error-and-trial method to determine the most suitable parameters,here,we present a machine learning(ML)-enabled image analysis-based method for determining the optimum MEW printing parameters through an easy-to-use graph-ical user interface(GUI).We trainedfive different ML algorithms using 168 MEW 3D print samples,among which the Gaussian process regression ML model yielded 93%accuracy of the variability in the dependent variable,0.12329 on root mean square error for the validation set and 0.015201 mean square error in predicting line *** of ML with a control feedback loop and MEW can reduce the error-and-trial steps prior to the 3D printing process,decreasing the printing time(i.e.,increasing the overall throughput of MEW)and material waste(i.e.,improving the cost-effectiveness of MEW).Moreover,embedding a trained ML model with the feedback control system in a GUI facilitates a more straightforward use of ML-based optimization techniques in the industrial section(i.e.,for users with no ML skills).

关键词： 3D printing additive manufacturing feedback control image analysis machine learning melt electrowriting optimization polymer

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