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The First Asian Conference on Plant Pathology

作者： AbdulMuinAdrian RusmilahSuseno SittiSutarmiTjitrosoma SoetrisnoHadi AbdurraufRambe SidartoWardojo Fakultas Pertanian Institut Pertanian Bogor Bogor Indonesia Fakultas Pertanian Institut Pertanian Bogor Bogor Indonesia Fakultas Matematika dan Iimu Pengetahuan Alam Institut Pertanan Bogor Bogor Indonesia Fakultas Kehutanan Institut Pertanian Bogor Bogor Indonesia Fakultas Matematika dan Iimu Pengetahuan Alam Institut Pertanan Bogor Bogor Indonesia Pusat Penelitian Perkebunan Bogor Bogor Indonesia.

＜正＞Inventory of fungal species colonizing root knot nematode （meloidogyne spp.） infecting soybean roots at four villages in West Java, was made. The infected roots were washed thoroughly by using autoclaved water, then soaked and shaken in 0.5％ NaOCI for 30 seconds, and immediately rinsed with autoclaved water. The adult female nematodes were extracted from the root tissues and subsequently rinsed with autoclaved water and then aseptically placed on water agar in Petri dishes. Five nematodes were placed in each Petri dish and subsequently incubated at room temperature for 2-3 days or longer to allow the development of the fungi colonizing nematodes. The fungal species developing from the individual nematode were transferred to Potato Dextrose Agar for identification. Out of the 265 nematodes collected, 40 individuals were colonized by different fungal species, of which 16 came from Wanajaya samples, 10 from Cipanas samples, 6 from Darmaga samples, and 8 from Sindangbarang samples. Among the isolates obtained, the fungal species that could be identified were Paecilomyces fumosoroseus, P. javanicus, Trichoderma viride, T. harzianum Aspergillus niger, A. parasiticus, A. fiavus, Gliocladium roseum, G. catenulatum and Fusarium fiocciferum. Some of them are suspected to be potential for controlling meloidogyne spp. on soybean.

关键词： meloidogyne spp. fungal species colonization soybean West Java

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Disease-resistant Mechanism of Pepper against Root-Knot Nematode(meloidogyne spp.)

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Plant Diseases and Pests 2021年第1期12卷 1-3页

作者： Miao Yue Liu Ziji Cao Zhenmu Zhu Jie College of Horticulture Hainan UniversityHaikou 570228China Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural SciencesHaikou 5711011 China

The biological characteristics,damage symptoms,pathogenic mechanism,prevention and control methods of meloidogyne *** pepper,as well as identification and study of pepper resistance against meloidogyne *** reviewed an... 详细信息

关键词： Pepper meloidogyne spp. Disease resistance

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Biochar from grape pomace,a waste of vitivinicultural origin,is effective for root-knot nematode control

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Biochar 2023年第1期5卷 519-541页

作者： Ángela Martínez-Gómez Maria Fe Andrés Ángel Barón-Sola Fernando E.Díaz-Manzano Ibraheem Yousef Ismael F.Mena Elena Díaz Óscar Gómez-Torres Azucena González-Coloma Luis E.Hernández Carolina Escobar Facultad de Ciencias Ambientales y Bioquímica Universidad de Castilla-La ManchaToledoSpain Departamento de Protección Vegetal Instituto Ciencias Agrarias CSICMadridSpain Departamento de Biología Universidad Autónoma de MadridMadridSpain CELLS ALBA Carrer del Llum 2-2608290Cerdanyola del VallèsBarcelonaSpain Departamento de Ingeniería Química Universidad Autónoma de MadridMadridSpain Faculty of Chemical Sciences&Technologies Chemical Engineering DepartmentAv.Camilo JoséCela1213071University of Castilla-La ManchaCiudad RealSpain

Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy *** development of new management strategies becomes essential as effective chemical nematicides are progressively being ***,we analysed grape pomace-derived biochars,pyrolysed at 350°C(BC350)and 700°C(BC700),focusing on their potential for RKN *** thermal treatment of grape pomace caused an increase in the concentration of carbon and plant macro-and micronutrients,which were largely present in a water-soluble *** radiation-based Fourier transform infrared microspectroscopy data showed a general loss of carboxylic functional groups during pyrolysis,partially contributing to the alkalinisation of both biochars,mostly in *** electron microscopy coupled with energydispersive X-ray spectroscopy analysis revealed a highly porous structure filled with different crystals composed of elements such as K,Ca,Mg,P,Si or Al,which could be a suitable environment for the growth of ***-derived aqueous extracts showed phytotoxicity to tomato seedlings at high concentrations,and disappeared upon dilution,but no toxic effect was observed on the nematode’s infective ***,the infective and reproductive traits of a meloidogyne javanica population in tomato were significantly reduced(*** masses and eggs per plant)in washed-biochar-treated soil in pots(0.75%;BC350W).Therefore,the large amount of grape waste generated after wine production can be transformed into a valuable product such as biochar,effective for RKNs control,thus reducing the waste management problem and contributing to a circular economy.

关键词： Biochar Grape pomace Tomato Root-knot nematode control meloidogyne spp.

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Evaluation of Essential Oils from Rosemary, Orange, Lavandula and False Yellowhead on Hatching and Motility of Root-Knot Nematode

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Journal of Agricultural Science and Technology(A) 2013年第8期3卷 603-616页

作者： Nikoletta G. Ntalli Eleni Nasiou Urania Menkissoglu-Spiroudi Pesticide Science Laboratory Faculty of Agriculture Aristotle University of Thessaloniki Thessaloniki 54124 Greece

Essential oils （EOs） from Rosemary, Rosmarinus officinalis （Lamiaceae）, Orange, Citrus sinensis （Rutaceae）, Lavandula, Lavandula angustifolia （Lamiaceae）, False Yellowhead, Dittrichia viscosa （Asteraceae） and their major components were evaluated against root knot nematode, meloidogyne incognita and meloidogynejavanica. Second stage juveniles＇（J2） paralysis and egg hatch inhibition were studied, while pulverized plant parts were tested for nematodes biological cycle arrest. All EOs paralyzed J2 and M. incognita were found more sensitive than M javanica with the EC50/4 days calculated at 250, 3,650 and 4,260 12g/mL for 1）. viscosa, L. angustifolia and R. officinalis, respectively. Similarly, all EOs inhibited nematodes egg hatch and D. viscosa exhibited the highest inhibition on egg hatch （100% inhibition at 5 μg/mL）. A significant influence of constituent terpenes （limonene, 1,8-cineole, linalool, camphor, L-borneol, caryophyllene oxide, β-eudesmol） dose and exposure time was indicated on egg hatch inhibition （56% to 100% at 500 μg/mL and 1,000 μg/mL）, while only β-eudesmol achieved paralysis of J2 and specifically against M. incognita （EC50/1d = 50μg/mL）. Interestingly, the most active botanical species arresting meloidogyne spp. biological cycle in soil was C. sinensis （EC50 = 2 mg/g） and the most sensitive nematode species was M. javanica. The larvicidal and egg hatch inhibition activity holds promise towards the optimization of artificial terpene mixtures as novel and effective natural nematicides. Complex interactions of primary compounds and subsequent decomposition derivates compose efficacy profile of soil amendments.

关键词： Nematicides biopesticides meloidogyne spp. Rosmarinus officinalis Citrus sinensis Lavandulaangustifolia Dittrichia viscose GC-MS.

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Selection of Biocontrol Trichoderma spp. against Root-knot Nematode and Preliminary Studies on the Strain of Snef 85

Selection of Biocontrol Trichoderma spp. against Root-knot N...

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中国植物病理学会2008年学术年会

The effective biocontrol strain Snef 85 was obtained by systemic screening against root-knot nematode(meloidogyne spp.). The strain Snef 85 was tested include the biocontrol character, optimum fermentation condition, physical and chemistry property of fermented broth, nematicidal and antimicrobial spectrum assay, green-house control effect and evaluation of safety to plant. The 160 strains of Trichoderma spp. were isolated from soil samples collected from different area and the 92 strains of Trichoderma spp. were obtained from the laboratory. The nematicidal activities of metabolites from those strains were tested. The results showed that there were 29 strains which had effect to meloidogyne spp. by primary screen. The biocontrol Trichoderma spp. Snef 85 was gained by fermentation screen which had effectively nematicidal toxin to meloidogyne spp. and genetic stability. The fermented broth of strain Snef 85 had poisonous effect on nematodes, and significant virulence to different nematodes was detected, the most poisonous effect on nematodes was meloidogyne spp., Aphelenchoides besseyi, Heterodera glycines, secondly was Ditylenchus destructor and Caenarhabditis spp. was the least. In dual-culture test, the inhibitory effect of strain Snef 85 against Bipolaris maydis was better and the inhibitory rate was 59.53%, the inhibitory effects of strain Snef 85 against Fusarium moniliforme and Botrytis cinerea were worse and the inhibitory rates were 37.75% and 37.61%, respectively. In cylinder plate tests, the fermented broth of strain Snef 85 had significant inhibitory effect to Bipolaris sorokiniana, Sclerotinia sclerotiorum, Exserohilum turcicum, Fusarium oxysporum. There were no inhibitory effect to Verticillium dahliae and Fusarium solani. Strain Snef 85 was identified as Trichoderma harzianum based on culture characteristics observation, morphological characteristics observation and the assay of ITS sequence. The biological characters of strain Snef 85 were studied. Th

关键词： meloidogyne spp. Trichoderma spp. metabolite biocontrol

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Research on Incidence Regularity and Ecological Control of Root-knot Nematode Disease

Research on Incidence Regularity and Ecological Control of R...

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中国植物病理学会2011年学术年会

作者：陈国康 College of Plant Protection Southwest University

＜正＞The investigation on the incidence regularity of root-knot nematode disease（Meloidogene spp.） was conducted in the ***,the research tried to explore probable way for ecological control of the soil borne disease caused by Meloidogene spp..The main results of the study were as follows. *** the special condition of agricultural cultivation system and the climate in area of Chongqing,the investigation on the incidence regularity of root-knot nematode disease was necessarily carried out in a typical agricultural base for vegetable growth.（1） It was found the disease Meloidogene *** tomato annually occurred at April in the *** disease incidence,whereafter,got more and more *** disease rate and index both increased dramatically in June,generally reached the top in July.（2） Analysis showed there was a correlationship between the disease rate of root-knot nematode on tomato and the average air *** the environmental temperature increasing,the disease index added,the disease rate attained 100%in July of the summer.（3） As contrast of two kinds of planting pattern,one was tomato（summer crop） -cabbage（winter crop）,the other was tomato（summer crop） - cucumber（winter crop）,the result showed the tomato planted after cabbage in the first pattern had a lower disease incidence than the second pattern infected by Meloidogene spp..It indicated the disease incidence could be decreased when cabbage,as a less susceptible host was grown before tomato was planted *** put a proof that the ecological agriculture could be applied to disease control.（4） It was observed in the research that tomato crop declined,discolored into yellow,wilted slowly when it’s just infected by Meloidogene spp.,while it’s both invaded by Meloidogene *** Corticium rolfsii,the plant wilted quickly even got dead *** was suggested these two types of diseases had a lethal threaten to tomato production. *** on the perineum vein characteristics of Meloido

关键词： meloidogyne spp. disease incidence pathogen identification nematodes community behavior pattern biological control fungi

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Genes expression related to Salicylic Acid Signaling Pathway in Overexpression Plants with MiPDCD6 in tomato

Genes expression related to Salicylic Acid Signaling Pathway...

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中国植物病理学会2021年学术年会

作者：邓小大 LI Yuan WU Luping KANG Zhiqiang YUAN Yongqiang 王新荣 Guangdong Province Key Laboratory of Microbial Signals and Disease Control College of Plant ProtectionSouth China Agricultural University

Root-knot nematodes（RKN,meloidogyne spp.） is one of the most destructive plantparasitic *** 98 species of RKN have been reported,which can invade more than 4000 plant species and seriously threatens global agricultural *** the process of parasitism,RKN secreted effectors into host plant root cells through the hollow stylet,suppress the host defense responses and induce the transformation of root cells into specialized hypertrophied and multinucleate feeding ***6,a programmed death protein 6（PDCD6） of meloidogyne incognita,is mainly secreted by the esophageal glands and interacts with R2 R3-MYB transcription factor LeMYB330 *** of MiPDCD6 gene in tomato could enhance plant susceptibility and promote RKN ***,silencing of MiPDCD6 gene by Virus-induced gene silencing（VIGS）method decreased the parasitic capacity of RKN and the number of root galls,the expression of PR1 gene was up-regulated,which is a marker gene of salicylic acid（SA） signal transduction ***330 protein is involved in the regulation of plant *** of LeMYB330 gene enhanced the susceptibility of tomato,significantly increased the numbers of root galls,and suppressed PR1 gene *** results may suggest that the suppression of plant defense response by MiPDCD6 is related to the SA signaling *** SA signal pathway plays an important role in plant PTI immune response,we hypotheses that MiPDCD6 may suppress tomato PTI through SA signaling ***,in our study we constructed overexpression plants with MiPDCD6 in tomato and analyzed their transcriptomes.A total of 2366 differentially expressed genes（DEGs） were identified in MiPDCD6 over-expression tomato plants,including 1354 up-regulated genes and 1012 down-regulated *** KEGG pathway related to plant immunity enriches a large amount of DEGs,including plant hormone signal transduction（sly04075）,plant-pathogen interaction（sly04626

关键词： meloidogyne spp. SA signaling pathway PTI immunity

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