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A meloidogyne incognita effector Minc03329 suppresses plant immunity and promotes parasitism

Journal of Integrative Agriculture 2023年第3期22卷 799-811页

作者： ZHOU Jing-jing ZHANG Xiao-ping LIU Rui LING Jian LI Yan YANG Yu-hong XIE Bing-yan ZHAO Jian-long MAO Zhen-chuan State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

meloidogyne incognita is a devastating plant-parasitic nematode.Effectors play important roles during the stages of nematodes infection and parasitism,but their molecular functions remain largely unknown.In this study,we characterized a new effector,Minc03329,which contains signal peptide for secretion and a C-type lectin domain.The yeast signal sequence trap experiments indicated that the signal peptide of Minc03329 is functional.In situ hybridization showed that Minc03329 was specifically expressed in the subventral esophageal gland.Real-time qPCR confirmed that the expression level of Minc03329 transcript was significantly increased in pre-parasitic and parasitic second-stage juveniles(pre-J2s and par-J2s).Tobacco rattle virus(TRV)-mediated gene silencing of Minc03329 in host plants largely reduced the pathogenicity of nematodes.On the contrary,ectopic expression of Minc03329 in Arabidopsis thaliana significantly increased plant susceptibility to nematodes.Transient expression of Minc03329 in Nicotiana benthamiana leaves suppressed the programmed cell death triggered by the pro-apoptotic protein BAX.Moreover,the transcriptome analysis of Minc03329-transgenic Arabidopsis and wild type revealed that many defense-related genes were significantly down-regulated.Interestingly,some different expressed genes were involved in the formation of nematode feeding sites.These results revealed that Minc03329 is an important effector for M.incognita,suppressing host defense response and promoting pathogenicity.

关键词： meloidogyne incognita effector C-type lectin pathogenicity plant immunity

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Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of Arachis hypogaea(Fabales:Fabaceae)against meloidogyne incognita(Tylenchida:Heteroderidae)

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生物细胞(英文) 2023年第9期47卷 2101-2113页

作者： XUEJIN YANG YUANYUAN ZHOU XINYI PENG XIAOHONG FU JIANQING MA JIANFENG LIU DANDAN CAO College of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaoding071002China Hebei Innovation Center for Bioengineering and Biotechnology Hebei UniversityBaoding071002China

Background:Plant root-knot nematode(RKN)disease is a serious threat to agricultural production across the world.meloidogyne incognita is the most prominent pathogen to the vegetables and cash crops cultivated.Arachis hypogaea can effectively inhibit M.incognita,but the underlying defense mechanism is still unclear.Methods:In our study,the chemotaxis and infestation of the second-stage juveniles(J2s)of M.incognita to A.hypogaea root tips were observed by the Pluronic F-127 system and stained with sodium hypochlorite acid fuchsin,respectively.The transcriptome data of A.hypogaea roots with non-infected or infected by J2s were analyzed.Results:The J2s could approach and infect inside of A.hypogaea root tips,and the chemotactic migration rate and infestation rate were 20.72%and 22.50%,respectively.Differential gene expression and pathway enrichment analyses revealed ubiquinone and other terpenoid-quinone biosynthesis pathway,plant hormone signal transduction pathway,and phenylpropanoid biosynthesis pathway in A.hypogaea roots responded to the infestation of M.incognita.Furthermore,the AhHPT gene,encoding homogentisate phytyltransferase,was considered to be an ideal candidate gene due to its higher expression based on the transcriptome data and quantitative real-time PCR analysis.Conclusion:Therefore,the key gene AhHPT might be involved in the A.hypogaea against M.incognita.These findings lay a foundation for revealing the molecular mechanism of A.hypogaea resistance to M.incognita and also provide a prerequisite for further gene function verification,aiming at RKN-resistant molecular breeding.

关键词： Arachis hypogaea meloidogyne incognita Transcriptome data AhHPT gene

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Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode meloidogyne incognita

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第3期12卷 219-225页

作者： Xiao-ning YAN Richard A. SIKORA Jing-wu ZHENG Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Soil-Ecosystem Phytopathology and Nematology Institute for Resource Conservation and Plant Science (INRES) Department of Plant Health University of Bonn Bonn 53115 Germany Institute of Environment and Plant Protection Chinese Academy of Tropical Agricultural Sciences Danzhou 5 7173 7 China

Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control.Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against meloidogyne incognita.Among the 294 isolates screened,23 significantly reduced galls formed by M.incognita in greenhouse test.The 10 most effective isolates were Fusarium(5),Trichoderma(1),Chaetomium(1),Acremonium(1),Paecilomyces(1),and Phyllosticta(1) .Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M.incognita were studied.They reduced the number of galls by 24.0%-58.4%in the first screening and 15.6%-44.3%in the repeated test,respectively.Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings.Fusarium isolates had colonization preference on the roots,their root colonizations ranging from 20.1%to 47.3%of the total root area.Trichoderma Tr882,Paecilomyces Pa972,and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts.Acremonium Ac985,Chaetomium Ch1001,Paecilomyces Pa972,and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M.incognita.Based on these results,Chae-tomium Ch1001 was considered to have the highest potential as a seed treatment agent for M.incognita biocontrol.

关键词： Cucumber endophytic fungi meloidogyne incognita Control efficacy Colonization In vitro activity

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Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of meloidogyne incognita on tomato

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Journal of Integrative Agriculture 2017年第6期16卷 1347-1357页

作者： JIN Na XUE Hui LI Wen-jing WANG Xue-yan LIU Qian LIU Shu-sen LIU Pei ZHAO Jian-lon JIAN Heng Department of Plant Pathology China Agricultural University/Key Laboratory of Plant Pathology Ministry of Agriculture Beijing 100193 P.R.China

Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of meloidogyne spp.,was evaluated as a potential biocontrol agent of meloidogyne incognita under in vitro and protective field.Microscopic observations showed that HDZ-9-47 parasitized eggs of M.incognita within 7 days.In vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles（J2s）of M.incognita and inhibited more than 50%egg hatching.In the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and methods.In addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.And tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control.

关键词： meloidogyne incognita Streptomyces rubrogriseus biocontrol parasitism nematicidal metabolites control efficacy

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Sources for Heat-Stable Resistance to Southern Root-Knot Nematode (meloidogyne incognita) in Solanum lycopersicum

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Agricultural Sciences in China 2009年第6期8卷 697-702页

作者： WU Wen-wen SHEN Huo-lin YANG Wen-cai Department of Vegetable Science College of Agronomy and Biotechnology China Agricultural University Beijing 100193 P.R.China

Southern root-knot nematode （meloidogyne incognita） is a major problem in vegetable production in China due to the expansion of plastic tunnel and solar greenhouse. Using resistant cultivars is an effective approach to control the disease. Nine genes, Mi-1 to Mi-9, have been reported and only Mi-1 has been successfully used in tomato breeding. However, Mi-1 is inactive at a temperature above 28~C. In order to identify sources for heat-stable resistance to southern root-knot nematode, 53 genotypes of tomato （Solarium spp.） were inoculated with an isolate of M. incognita in the growth chamber at 28 or 32℃ for initial screening. 28 lines had less than 25 galls and were considered as resistant candidates. The top 60% （16 in total） of resistant candidates obtained from each temperature were subject to re-evaluation at 32~C using the same nematode isolate. Three lines ZN17, ZN 48, and LA0385 showed heat-stable resistance with an average of 10 galls or less per plant. LA0385 is a wild species, while ZNI7 and ZN48 are elite breeding lines. These lines were grown in a greenhouse for two seasons, and also showed high resistance with less than 10 galls per plant. Thus they were considered as good sources for breeding resistance to southern root-knot nematode in tomato.

关键词： heat-stable resistance meloidogyne incognita tomato

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Associated bacteria of different life stages of meloidogyne incognita using pyrosequencing-based analysis

Associated bacteria of different life stages of Meloidogyne ...

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中国烟草学会2015年年会

作者： Yi Cao Baoyu Tian Xinglai Ji Shenghua Shang Chaojun Lu Keqin Zhang Key Laboratory for Conservation and Utilization of Bio-resource and Key Laboratory for Microbial Resources of the Ministry of EducationYunnan University Key Laboratory of Molecular Genetics Guizhou Academy of Tobacco Science College of Life Science Fujian Normal University

The root knot nematode(RKN), meloidogyne incognita, belongs to the most damaging plant pathogens worldwide, and is able to infect almost all cultivated plants, like tomato. Recent research supports the hypothesis that bacteria often associated with plant-parasitic nematodes,function as nematode parasites, symbionts, or commensal organisms etc. In this study, we explored the bacterial consortia associated with M. incognita at different developmental stages,including egg mass, adult female and second-stage juvenile using the pyrosequencing approach.The results showed that Proteobacteria, with a proportion of 71–84%, is the most abundant phylum associated with M. incognita in infected tomato roots, followed by Actinobacteria,Bacteroidetes, Firmicutes etc. Egg mass, female and second-stage juvenile of M. incognita harbored a core microbiome with minor difference in communities and diversities. Several bacteria genera identified in M. incognita are recognized cellulosic microorganisms, pathogenic bacteria, nitrogenfixing bacteria and antagonists to M. incognita. Some genera previously identified in other plantparasitic nematodes were also found in tomato RKNs. The potential biological control microorganisms, including the known bacterial pathogens and nematode antagonists, such as Actinomycetes and Pseudomonas, showed the largest diversity and proportion in egg mass, and dramatically decreased in second-stage juvenile and female of M. incognita. This is the first comprehensive report of bacterial flora associated with the RKN identified by pyrosequencingbased analysis. The results provide valuable information for understanding nematode–microbiota interactions and may be helpful in the development of novel nematode-control strategies.

关键词： meloidogyne incognita Biological control Nematode-associated bacterial consortia Microbial diversity Pyrosequencing

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Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode

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Horticultural Plant Journal 2023年第1期9卷 133-144页

作者： Yang Liu Hongyi Cao Jian Ling Yuhong Yang Yan Li Bingyan Xie Jianlong Zhao Zhenchuan Mao Institute of Vegetables and Flowers Chinese Academy of Agricultural ScienceBeijing 100081China

Root-knot nematodes(RKNs)cause severe diseases in peppers annually around the world.In pepper,the Me3 gene provides a heat-stable and broad-spectrum resistance to RKNs.In this study,several simple sequence repeat(SSR)markers and insertion/deletion(In Del)markers were developed to fine map the Me3 gene.Analysis of 2272 individuals(F2progenies)revealed that Me3 was located in a 45-kb DNA region between markers SSR784 and SSR339,in which there were three candidate genes.Among them,as a novel nucleotide binding site and leucine rich repeat(NBS-LRR)family gene,the DNA sequence of Capana09g000163 of pepper line‘HDA149’was 6348 bp in length,with a 2802-bp open reading frame encoding 933 amino acids,including NB-ARC and LRR domains.Tobacco transient expression assays demonstrated that expression of Capana09g000163 triggered a hypersensitive response(HR)in Nicotiana benthamiana leaves.Subcellular localization results showed that the Capana09g000163 protein was localized in the cell nucleus.Ectopic expression of Capana09g000163 in Arabidopsis significantly increased resistance against meloidogyne incognita compared with the wild-type(WT)Arabidopsis.Furthermore,M.incognita was almost unable to develop in transgenic Arabidopsis expressing Capana09g000163.Taken together,we cloned the Me3 gene and verified that it induced resistance against M.incognita with the methods of map-based cloning and transgenic technology,which may be of great significance to pepper breeding for resistance against RKNs.

关键词： Capsicum annuum L meloidogyne incognita Nematode-resistant gene Fine mapping Transgenic technology

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桑根受南方根结线虫(meloidogyne incognita)侵染后的组织病理学研究

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中国蚕业 1986年第1期 57-57页

作者： MD.SamsulHaque 许晓风

Narayanan等(1966)在迈所(Mysore)第一次记录了桑树根结线虫的发生情况。1976年Haque在印度北汉坡(Berhampore)和西阪哥(West Bengal)的调查过程中,发现桑根受南方根结线虫(M.incogtina)的侵染严重。但迄今为止关于根结线虫对桑树的病... 详细信息

Narayanan等(1966)在迈所(Mysore)第一次记录了桑树根结线虫的发生情况。1976年Haque在印度北汉坡(Berhampore)和西阪哥(West Bengal)的调查过程中,发现桑根受南方根结线虫(M.incogtina)的侵染严重。但迄今为止关于根结线虫对桑树的病理影响几乎未见报道过。本研究旨在观察由南方根结线虫引起的桑根组织的组织病理学变化。材料和方法将受线虫侵染的以及健康的当地栽培的

关键词：南方根结线虫南方根癌线虫桑根 meloidogyne incognita 侵染组织病理学病理组织学组织学

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Synthesis, nematicidal activity and docking study of novel chromone derivatives containing substituted pyrazole

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Chinese Chemical Letters 2018年第6期29卷 911-914页

作者： Wei Li Jiuhui Li Hongfeng Shen Jiagao Cheng Zhong Li Xiaoyong Xu Shanghai Key Laboratory of Chemical Biology School of Pharmacy East China University of Science and Technology Shanghai 200237 China Shanghai Collaborative innovation Center for Biomanufacturing Technology Shanghai 20023F China

A series of chromone derivatives containing substituted pyrazole were designed and synthesized.Preliminary bioassays showed that most of the synthesized compounds exhibited good nematicidal activity in vivo against meloidogyne incognita at 10 mg/L. Among the tested compounds, A10 and A11 exhibited 100% inhibition rates. In addition, the molecular docking results indicated that both compound A10 and A11 interacts with amino acid residue Tyr121, Trp279, Tyr70, Trp84 and Phe330 of ACh E via hydrogen bond and p–p stacking. This investigation suggested that the chromone containing substituted pyrazole scaffold could be further optimized to explore novel, high-bioactivity nematicidal leads.

关键词： Chromone Substituted pyrazole meloidogyne incognita Nematicidal activity Molecular docking

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Improvement of Bio-Efficacy to Reduce Bacterial Wilt Complex Disease in Tomatoes through Bacillus amyloliquefaciens and Pseudomonas fluorescens under Field Conditions

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Journal of Agricultural Science and Technology(B) 2021年第3期11卷 109-120页

作者： Dinesh Singh Dhananajay Kumar Yadav Harender Kumar Sharma Garima Chaudhary Division of Plant Pathology ICAR-Indian Agricultural Research InstituteNew Delhi 110012India Division of Nematology ICAR-Indian Agricultural Research InstituteNew Delhi 110012India

Bacterial wilt complex disease of tomato(Solanum lycopersicum L.)was incited jointly by bacterial wilt pathogen Ralstonia solanacearum and meloidogyne incognita worldwide.Bio-efficacy of bacterial antagonists i.e.B.amyloliquefaciens DSBA-11 and P.fluorescens DTPF-3 was studied against the wilt disease complex in tomato at National Phytotran facility Indian Agricultural Research Institute(IARI),New Delhi,at 26±2°C.Minimum wilt disease incidence(26.00%)with the highest bio-control efficacy(64.15%),less juvenile population(19.33 J2/g of soil)of M.incognita was recorded in the combined application of DTPF-3+DSBA-11 after 30 d of inoculation under glasshouse conditions.In a field study,minimum bacterial wilt disease incidences 19.0%and 20.4%were recorded in the bleaching powder treatment followed by mixed application of DSBA-11+DTPF-3,19.6%and 21.2%wilt incidence in 2014 and 2015 respectively.However,a reduction of root-knot gall index was recorded a maximum of 59.76%and 69.62%in DSBA-11+DTPF-3 treated plants followed by 54.88%and 60.13%over control in DTPF-3 treatment in 2014 and 2015 respectively.The yield of tomato fruit was increased over control by 17.48%and 16.97%in 2014 and 2015 respectively under field conditions.A combination of P.fluorescens DTPF-3+B.amyloliquefaciens DSBA-11 suppressed bacterial wilt and root-knot diseases and also increased the yield of the tomato fruit significantly(p<0.05)under field conditions.

关键词： Bacillus amyloliquefaciens bacterial wilt complex meloidogyne incognita Ralstonia solanacearum Pseudomonas fluorescens tomato

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