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Contribution of molecular cladistics to the taxonomy of Rutaceae in China

Journal of Systematics and Evolution 2009年第2期47卷 144-150页

作者： Paul Pui-Hay BUT Alice Wing-Sem POON Pang-Chui SHAW Mark P.SIMMONS Harald GREGER Department of Biology Chinese University of Hong Kong Department of Biochemistry Chinese University of Hong Kong Department of Biology Colorado State UniversityFort Collins Comparative and Ecological Phytochemistry Faculty Center of BotanyUniversity of Vienna

The treatment of Rutaceae in the Chinese flora chose to follow Engler in recognizing Rutoideae and Toddalioideae as two separate subfamilies.Morphological and chemical comparisons,however,suggested group-ing those two subfamilies in one subfamily,Rutoideae.This move has received support from molecular phyloge-netic analyses,which also showed that the Chinese taxa in Euodia should be placed in Tetradium and melicope following Hartley.Investigations into the chemistry and molecular phylogeny of Murraya also indicated that the species in the section Bergera without yuehchukene should be removed from Murraya.These findings clearly show the value of molecular cladistics to the taxonomy of Rutaceae in China and also directions for further inves-tigations.

关键词： Bergera Euodia melicope Murraya Rutaceae Rutoideae Tetradium Toddalioideae

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Direct Shoot Regeneration from Callus of <i>melicope lunu-ankenda</i>

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Natural Science 2015年第2期7卷 81-87页

作者： Ab. Zuraida Rahman Ayu Nazreena Othman Fatin Liyana Izzati Kamaruddin Aziz Bin Ahmad Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Universiti Malaysia Terengganu Kuala Nerus Malaysia Centre for Fundamental and Liberal Education Universiti Malaysia Terengganu Kuala Nerus Malaysia

melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.

关键词： melicope lunu-ankenda Regeneration Plant Growth Regulator In Vitro

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