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Selection of culture conditions for callus induction and proliferation by somatic embryogenesis of Pinus koraiensis

Journal of Forestry Research 2021年第2期32卷 483-491页

作者： Fang Gao Chunxue Peng Hao Wang Hailong Shen Ling Yang State Key Laboratory of Tree Genetics and Breeding School of ForestryNortheast Forestry UniversityHarbin 150040People’s Republic of China

The induction and proliferation of embryogenic callus are key steps for large-scale propagation of somatic embryogenesis pathway and long-term preservation of coniferous *** can be induced from immature embryos of Korean pine(Pinus koraiensis *** Zucc.;Pinaceae)as explants,but there are problems,such as low proliferation efficiency,loss of embryogenicity,poor vigor;thus,best conditions for proliferation and culture of immature embryos of Korean pine are not yet *** solve the problems with somatic embryogenesis of Korean pine and determine the best culture conditions for callus induction and proliferation,we varied hormone concentration,subculture cycle of proliferation and other plant growth regulators combinations in media to induce callus formation by megagametophytes of three Korean pine families at different developmental stages,then analyzed the effects on embryogenic callus retention and cell proliferation using a quadratic regression orthogonal rotation *** results showed that the family origin and collection date of explants significantly affected callus induction(induction rate reached 1.67%).Embryogenic maintenance and callus proliferation were best on DCR medium supplemented with 0.25 mg L^(−1)6-benzyl adenine,1 mg L^(−1)naphthaleneacetic acid,30 g L^(−1)sucrose,500 mg L^(−1),L-glutamine,500 mg L^(−1)casein hydrolysis and 6.5 g L^(−1)*** addition,the combination of 2,4-dichlorophenoxyacetic acid+6-benzyl adenine also had a better proliferative effect on *** effects of different combinations of growth regulators on callus proliferation efficiency were significantly *** to new medium every 13–15 days not only maintained robust callus vigor,but also yielded a larger proliferation *** techniques and conditions for embryogenic callus induction and proliferation of Korean determined here will serve as a foundation for establishing a large-scale system for somatic embryogenesis and propagation of

关键词： Korean pine Somatic embryogenesis Embryogenic callus megagametophytes Embryonic maintenance

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