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Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application

Journal of Oceanology and Limnology 2021年第6期39卷 2107-2115页

作者： Changyou CHEN Pingping WANG Long-Fei WU Tao SONG Beijing Key Laboratory of Bioelectromagnetism Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms Chinese Academy of SciencesBeijing 100029China Aix Marseille University CNRSLCBMarseille F-13402France University of the Chinese Academy of Sciences Beijing 100049China

Magnetotactic bacteria are capable of biosynthesizing magnetic nanoparticles,also called magnetosomes,and swimming along magnetic field *** abilities endow the whole cells of magnetotactic bacteria with such applications as targeted therapy and manipulation of *** have shown that the intact marine magnetotactic bacteria MO-1 kill efficiently antibiotic-resistant pathogen Staphylococcus aureus in vivo,but the biocompatibility of this marine bacterium is *** this study,the strain MO-1 was chosen to analyze its biocompatibility and potential for biomedicine *** showed that MO-1 cells could be guided at 37℃ under an external magnetic field and swim in the blood plasma and *** could keep active locomotivity within 40 min in the plasma and urine,although their velocity slowed *** incubated with human cells,magnetotactic bacteria MO-1 had no obvious effects on cellular viability at low dose,while the cell toxicity increased with the augmentation of the quantity of the MO-1 cells *** the in-vivo experiments,the median lethal dose of magnetotactic bacteria MO-1 in rats was determined to be 7.9×10^(10) bacteria/*** results provided the foundation for the biocompatibility and safety evaluations of magnetotactic bacteria MO-1 and suggested that they could be basically used in clinical targeted therapy.

关键词： magnetotactic bacteria motility cell interaction median lethal dose

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Article Preclinical evaluation of acute systemic toxicity of magnesium incorporated poly(lactic-co-glycolic acid)porous scaffolds by three-dimensional printing Jing

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Biomaterials Translational 2021年第3期2卷 272-284,I0001页

作者： Jing Long Bin Teng Wei Zhang Long Li Ming Zhang Yingqi Chen Zhenyu Yao Xiangbo Meng Xinluan Wang Ling Qin Yuxiao Lai Centre for Translational Medicine and Research and Development Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Centre for Energy Metabolism and Reproduction Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Engineering Laboratory of Biomaterials Additive Manufacturing GuangdongGuangdong ProvinceChina Department of Orthopaedics and Traumatology The Chinese University of Hong KongHong Kong Special Administrative RegionChina Key Laboratory of Health Informatics Chinese Academy of SciencesShenzhenGuangdong ProvinceChina Centre for Translational Medicine and Research and Development Institute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

Biodegradable polymer scaffolds combined with bioactive components which accelerate osteogenesis and angiogenesis have promise for use in clinical bone defect *** preclinical acute toxicity evaluation is an essential assay of implantable biomaterials to assess the biosafety for accelerating clinical *** have successfully developed magnesium(Mg)particles and beta-tricalcium phosphate(β-TCP)for incorporation into poly(lactic-co-glycolic acid)(PLGA)porous composite scaffolds(PTM)using low-temperature rapid prototyping three-dimensional-printing *** PTM scaffolds have been fully evaluated and found to exhibit excellent osteogenic capacity for bone defect *** preclinical evaluation of acute systemic toxicities is essential and important for development of porous scaffolds to facilitate their clinical *** this study,acute systemic toxicity of the PTM scaffolds was evaluated in mice by intraperitoneal injection of the extract solutions of the *** composite scaffolds with different Mg andβ-TCP content(denoted as PT5M,PT10M,and PT15M)were extracted with different tissue culture media,including normal saline,phosphate-buffered saline,and serum-free minimum essential medium,to create the extract *** evaluation was carried out following the National *** acute toxicity was fully evaluated through the collection of extensive data,including serum/organs ion concentration,fluorescence staining,and in vivo median lethal dose *** in major organs(heart,liver,and lung),and Mg ion concentrations in serum of mice,after intraperitoneal injection of the extract solutions,were measured and showed that the extract solutions of PT15M caused significant elevation of serum Mg ion concentrations,which exceeded the safety threshold and led to the death of the *** contrast,the extract solutions of PT5M and PT10M scaffolds did not cause the death of the injected *** median lethal dose of Mg ions in vivo

关键词： acute systemic toxicity clinical translation magnesium median lethal dose porous composite scaffolds

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Estimation of cytotoxic potency by brine shrimp lethality bioassay application of Clerodendrum infortunatum Linn.

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Journal of Coastal Life Medicine 2015年第8期 636-639页

作者： Talukdar Muhammad Waliullah Akter Mst Yeasmin Ashraful Md Alam Wahedul Md Islam Parvez Hassan Molecular Cellular Biology Laboratory Bioscience DepartmentGSSTShizuoka University836-OyaSuruga-kuShizuoka 422-8529Japan FIQC Laboratory Department of FisheriesMatshya BhabanRamnaDhaka-1000Bangladesh Institute of Biological Sciences University of RajshahiRajshahi-6205Bangladesh

Objective:To learn a scientific and systematic knowledge of anticancer,antimicrobial and pharmacological activities of natural products and estimate cytotoxic potency by using ethanol and chloroform extracts of root,leaf and stem of Clerodendrum infortunatum(Verbenaceae)due to its random use in customary and traditional medicine to cure common ailments such as intestinal disorder,diarrhea,tuberculosis and respiratory problems ***:The in vitro application was carried out with the bench-top bioassay method by using brine shrimp lethality ***:All of the crude extracts were found to be lethal and *** LC50 value of ethyl alcohol fraction of root was 20.845 mg/L compared to the standard drug tetracycline of 14.675 mg/L to brine shrimp nauplii,indicating that the extracts were biologically ***:The cytotoxic study of LC50 value showed that a good correlation with the antibiotic *** the comparative correlation error bars and percentage,we understood that ethyl alcohol fraction of root extract was very *** study serves as a basis for further research to lead compounds to be isolated so that it may be as a template for the implications of these results for bioactivity and drug discovery potential of herbal products.

关键词： Plant Ethnobotanical information median lethal dose Cytotoxicity Artemia salina Standard deviation

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Acute Oral Toxicity Studies of a Chinese Herbal Preparation Shenrong Bunao Capsule

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Agricultural Biotechnology 2017年第3期6卷 62-63页

作者： Ping LI Xiangling QU Sibu MA Ming LIU The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine Guiyang College of Traditional Chinese Medicine

[ Objectives] This study was conducted to evaluate the acute oral toxicity of Shenrong Bunao Capsule, and to provide a theoretical basis for drug devel- opmcn! and clinical trims. [ Methods] Kunming mice were randomly assigned into two groups： an experimental group and a control group. In experimental group, Shenrong Bunao Capsule solution at maximum concentration of 45% was administered orally by gavage once at a dose of 30 ml/kg body weight （ BW）, while an equal volume of saline solution was given in the control group. Then, the appearance, behavior, mental state, diet, feces, urine, coat, skin color, respiration, nose, eyes and oral secretions and other daily activities of the mice were observed for 14 d after drug administration. At the end of the experiment, the mice were dissected, and their main organs were examined histopathologically. [Results] The appearance, behavior, respiration, body posture, response to stimuli and weight gain of the mice in control group were all normal. All the mice given 13. 500 g/kg BW Shenrong Bunao Capsule were alive 14 d after drug administration, and their appearance, behavior, respiration, body posture, response to stimuli and weight gains did not show any abnormality. In addition, the body weights of male and female mice had no obvious difference between the experimental group and the control group at the binging, 7 and 14 d after drug administration （P 〈0.05）. [ Conclusions] A maximum dose 13. 500 g/kg BW Shenrong Bunao Capsule, which was equivalent to 141 times of the recommended dosage for adult men was given to the mice, and did not result in any apparent toxic effects, suggesting that the Chines herbal preparation Shenrong Bunao Capsule has very little acute toxicity, and the commonly recommended dosage in clinical trials is safe.

关键词： Shenrong Bunao Capsule Acute toxicity test median lethal dose Maximum dose

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Separation of Molds in Contaminated Fruits and Toxicity Test

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Agricultural Biotechnology 2020年第6期9卷 108-113页

作者： Qing'e LIU Lishui University Lishui 323000China

[Objectives]This study was conducted to investigate the toxicity of molds in contaminative fruits.[Methods]Common fruits were used as materials to isolate contamination molds and screen the most virulent strain to study its pathogenic mechanism.[Results]We isolated eight kinds of strains from red bayberries,pears,oranges and peaches,and the P2 strain from red bayberries was the most virulent strain,which was preliminarily identified as Aspergillus *** toxin from the strain led to the bone marrow cell micronucleus phenomenon,and the greater the toxin dose,the greater the micronucleus *** the extension of time,the micronucleus rate *** SOD activity of the kidney was inhibited after the mice were exposed to the toxin,and the greater the toxin dose,the lower the SOD *** the extension of time,the SOD activity decreased,but increased instead after 48 h because the toxin was metabolized in the *** was always lower than that of control *** MDA content in kidney was also affected after the exposure,and the greater the toxin dose,the higher the MDA ***,it increased with the time prolonged and began to decrease until 48 h,but not less than the control *** indicated mold contamination in fruits produced toxins,and the P2 strain was the most toxic in red bayberries,causing chromosome and kidney injury.[Conclusions]This study provides a theoretical basis for the isolation and identification of molds in different fruits and toxicity tests,which is helpful to enhance people’s understanding of mycotoxins and their harm.

关键词： Mycotoxin median lethal dose Micronucleus Cell apoptosis Super oxide dixmutase

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