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Phosphoinositide and Inositolpolyphosphate Signalling in Defense Responses of Arabidopsis thaliana Challenged by mechanical wounding

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Molecular Plant 2008年第2期1卷 249-261页

作者： Alina Mosblech Sabine Konig Irene Stenzel Peter Grzeganek Ivo Feussner Ingo Heilmann Department of Plant Biochemistry Albrecht-yon-Hailer-Institute for Plant Sciences Georg-August-University G6ttingen Justus-von-Liebig-Weg 1137077 G6ttingen Germany

Various biochemical signals are implicated in Arabidopsis wound signalling, including jasmonic acid （JA）, salicylic acid, auxin, and Ca^2＋. Here, we report on cross-talk of phytohormones with phosphoinositide signals not previously implicated in plant wound responses. Within 30 min of mechanical wounding of Arabidopsis rosette-leaves, the levels of the lipid-derived soluble inositolpolyphosphate, inositol 1,4,5-trisphosphate （InsP3）, increased four to five-fold. Concom- itantly, the precursor lipids, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol transiently depleted, followed by re-synthesis after 30-60 min of stimulation. Increased InsP3 levels with wounding coincided with JA increases over the first hours of stimulation. In dde2-2-mutant plants deficient in JA biosynthesis, no InsP3 increase was observed upon wounding, indicating that JA was required for InsP3 formation, and InsP3 levels increased in wild-type plants challenged with sorbitol, increasing endogenous JA levels. In InsP 5-ptase plants with attenuated phosphoinositide signalling, the induction of wounding-inducible genes was diminished compared with wildtype plants, suggesting a role for phosphoinositide signalling in mediating plant wound responses. The gene-expression patterns suggest that phosphoinositides contribute to both JA-dependent and JA-independent aspects of wound signalling. Weight gain of Plutella xylostella caterpillars feeding on InsP 5-ptase plants was increased compared with that of caterpillars feeding on wild-type plants. The ecophysiological relevance of phosphoinositide signals in plant defense responses to herbivory is discussed in light of recent findings of inositolpolyphosphate involvement in phytohormone-receptor function.

关键词： Phosphoinositide and Inositolpolyphosphate Signalling Arabidopsis mechanical wounding

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Localized Effects of mechanical wounding and Exogenous Jasmonic Acid on the Induction of Secondary Laticifer Differentiation in Relation to the Distribution of Jasmonic Acid in Hevea brasiliensis

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Acta Botanica Sinica 2003年第11期45卷 1366-1372页

作者：田维敏史敏晶于凤义吴继林郝秉中崔克明北京大学生命科学学院北京100871 华南热带农业大学农学院儋州571737 中国农业科学院原子能利用研究所北京100094 中国热带农业科学院橡胶栽培研究所热带作物栽培生理学农业部重点开放实验室儋州571737

The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated with the aid of experimental morphological and radioisotope technique. Most radioactivity of H-3-JA sustained in treated site within one hour while no radioactivity was detected in new shoot and the radioactivity in upper leaf was much less than that in the parts below the treated site, suggesting that JA was mainly transported downwards in the shoot of H brasiliensis. mechanical wounding hindered the entrance of exogenous JA remarkably while held back the entered JA to the regions around wounded site. The effect of exogenous JA and mechanical wounding on the induction of the secondary laticifer differentiation was limited to treated site where high level of JA was expected. mechanical wounding reduced the effect of exogenous JA on the differentiation of secondary laticifer, which could be ascribed to the hindrance of mechanical wounding to the entrance of exogenous JA. It was concluded from the combined data that a high accumulation of JA was required for inducing the secondary laticifer differentiation in H. brasiliensis.

关键词： Hevea brasiliensis jasmonic acid mechanical wounding radioisotope technique laticifer differentiation

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Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 443-450页

作者： Yu-ying ZHAO Chun-lu QIAN Ji-cheng CHEN Yan PENG Lin-chun MAO Department of Food Science and Nutrition College of Biosystem Engineering and Food Seience Zhejiang University Hangzhou 310029 China College of Horticulture and Gardening Yangtze University Jingzhou 434025 China

This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be

关键词： Cucumber mechanical wounding Phospholipase D Lipoxygenase Phospholipid

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Response of Endogenous Salicylic Acid and Jasmonates to mechanical wounding in Pea Leaves

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Agricultural Sciences in China 2008年第5期7卷 622-629页

作者： LIU Yan PAN Qiu-hong ZHAN Ji-cheng TIAN Rong-rong HUANG Wei-dong College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 P.R.China College of Agronomy Inner Mongolia Agricultural University Huhhot 010019 P.R.China

The roles of on endogenous jasmonates （JAs） and salicylic acid （SA） in wounding response were investigated. Pea （Pisum sativum L.） seedlings were treated with three different methods including mechanical wounding, JAs application, and SA application. The contents of endogenous JAs and SA, as well as the activities of the related enzymes were detected by enzyme-linked immunosorbent assay （ELISA）, high performance liquid chromatography （HPLC）, and spectrophotometer, respectively. The results showed that endogenous JA rapidly accumulated within 30 min after wounding. The increase in the activities of both lipoxygenase （LOX） and allene oxide synthase （AOS） lagged behind JAs burst. A second slight increase in JAs level was observed at 24 h after wounding treatment, and at the same time point, higher activities of LOX and AOS were also detected. Endogenous free SA content decreased accompanied with JAs burst. Effects of exogenous JA application were similar to those of wounding treatment on endogenous SA level and phenylalanine ammonia lyase （PAL） activity, whereas exogenous SA application led to the significant inhibition of LOX and AOS activities and the decrease of endogenous JAs level at the early stage of treatment. It is thus suggested that JAs burst and SA decrease in early response to wounding may constitute an important mechanism by which plant starts the related defense reaction and adapts to wounding stress.

关键词： salicylic acid jasmonates mechanical wounding pea (Pisum sativum L.)

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