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MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle

生物细胞(英文) 2023年第9期47卷 2115-2123页

作者： MINGHONG WEI CHENG XIAO JIAN WU LIHONG QIN HONGLIANG LIU YANG CAO YUMIN ZHAO Institute of Animal Biotechnology Jilin Academy of Agricultural SciencesGongzhuling136100China Institute of Muscle Biology and Growth Research Institute for Farm Animal Biology(FBN)Dummerstorf18196Germany Key Laboratory of Beef Cattle Genetics and Breeding Ministry of Agriculture and Rural AffairsGongzhuling136100China

Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality traits.Mediator Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride deposition.However,the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown.Methods:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle.The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting.Results:The MED4 gene had significantly high expression during preadipocyte differentiation(p<0.05).Overexpression of the MED4 gene increased the expression of the PPARγgene,a preadipocyte differentiation biomarker,improved cellular lipid droplets and triglycerides accumulation,and positively accelerated adipocyte maturation(p<0.05).Interference of the MED4 gene can negatively regulate preadipocyte differentiation.Conclusion:This study showed that the MED4 gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the PPARγgene.

关键词： Chinese red steppe cattle MED4 PPARγ Predipocyte differentiation Lipid metabolism meat quality trait

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A genome-wide association study of five meat quality traits in Yorkshire pigs

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Frontiers of Agricultural Science and Engineering 2014年第2期1卷 137-143页

作者： Qian DONG Huiying LIU Xinyun LI Wei WEI Shuhong ZHAO Jianhua CAO Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of EducationCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China

meat quality is an important trait in the pig industry.To identify genomic regions and haplotype blocks responsible for meat quality traits in pigs,a genome-wide association study was conducted for five traits including intramuscular fat content,pH at 45 min and 24 h,drip loss within 24 h and water-holding capacity in 231 Yorkshire barrows using illumina porcine 60k SNP chips.The results showed that a total of 344 single nucleotide polymorphisms(SNP)were significantly associated with five meat quality traits(Pmeat quality related genes.In addition,25 haplotype blocks based on 116 SNPs were revealed with SNP combination patterns for five traits.Our study added new SNP information for identification of meat quality traits in pigs and will help elucidate the mechanisms of meat quality in pigs.

关键词： pig GWAS meat quality trait SNP

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