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High temperature induced masculinization of zebrafish by down-regulation of sox9b and esr1 via DNA methylation

Journal of Environmental Sciences 2021年第9期33卷 160-170页

作者： Jiangyuan Han Yan Hu Yongmei Qi Cong Yuan Sajid Naeem Dejun Huang Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution School of Life SciencesLanzhou UniversityLanzhou 730000China.

Elevated temperature could influence the sex differentiation by altering the expression of sex-related genes in ***, the underlying mechanisms by which the gene expression is altered remain poorly ***, we aimed to explore the role of DNA methylation in sex differentiation of zebrafish(Danio rerio) in response to elevated *** results showed that high temperature(33℃) exposure of fish from 20 to 30 days post fertilization(dpf), compared to normal temperature(28℃), resulted in male-biased sex ratio and decreased expression of female-related genes including cyp19a1a, sox9b and ***, the expressions of DNA methyltransferases dnmt3a1 and dnmt3a2, and the DNA methylation levels in sox9b and esr1 promoter were significantly increased by high temperature, strongly implying that DNA methylation is involved in high temperature-induced masculinization of ***-treatment with 5-aza-2-deoxycytidine(a DNA methylation inhibitor) attenuated the high temperature-induced masculinizing effect, recovered the expression of esr1 and sox9b, suppressed the transcription of dnmt3a1 and dnmt3a2, and decreased the methylation of esr1 and sox9b promoter, further confirming that DNA methylation plays an important role in high temperature-induced masculinization of ***, the methylation of sox9b promoter decreased the enrichment of transcription factor CREB(cAMP-responsive element binding proteins).Overall, these findings suggest that high temperature induce masculinization of zebrafish by down-regulation of femalerelated genes via DNA methylation, providing a new insight in understanding the epigenetic mechanism of thermal-mediated sex differentiation in fish.

关键词： Temperature Sex ratio masculinization DNA methylation

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Temperature-control-induced masculinization in tiger puffer Takifugu rubripes

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Journal of Oceanology and Limnology 2019年第3期37卷 1125-1135页

作者： ZHOU He ZHUANG Zixin ZHANG Rui XU Qizheng LIANG Yuting JIANG Zhiqiang LI Xia MA Tianyu LI Yajuan Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea Ministry of Agriculture Dalian Ocean University

This study used tiger puffer Takifugu rubripes to explore new,simple methods for lowtemperature-induced masculinization in a cultured fish without the use of ultraviolet irradiation or sex *** orthogonal test L9(3^4)design was used to consider three factors at three levels:treatment starting times(days post-hatch,dph:factor A)of 20,50 and 80 dph;treatment temperatures(factor B)of 13℃,15℃and 17℃;and treatment durations(factor C)of 30,45 and 60 days.A control group was reared at 21±1℃.The experiments were repeated *** 230 dph,the gonads were removed from thirty randomly sampled fish in each *** observations and analysis of single nucleotide polymorphisms(SNP)were used to identified pseudo males,which biological sex was male and genetic sex were female(XX).Treatment group 4(A2B1C2)resulted in the highest proportion of males(75%).According to the intuitive analysis of the orthogonal-array experiments,the optimal combination of lowtemperature-induced masculinization of *** was *** population sex ratio depended on the three factors in the sequence B→A→C.A comparison of the daily increases in length and weight during and after the low-temperature conditions showed that the absolute daily increases in weight and length were significantly less during treatment than after *** increases in weight and length did not significantly differ between the treatment groups and the controls(P>0.05),demonstrating that the growth rate could return to normal after the low-temperature *** study establishes a low-temperatureinduced masculinization technology for *** and demonstrates that although the growth rate(length and weight)decreased in an array of nine treatment groups during the processing time,it returned to a normal level after *** results should serve as a guide for achieving the masculinization of *** in production.

关键词： Takifugu rubripes temperature control masculinization orthogonal test growth rate

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The optimum dose and period of 17α-methyltestosterone immersion on masculinization of red tilapia(Oreochromis spp.)

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Aquaculture and Fisheries 2023年第2期8卷 174-179页

作者： Thuchapol Karaket Aisawan Reungkhajorn Pattareeya Ponza Fisheries Science Program Department of Agricultural ScienceFaculty of Agriculture Natural Resources and EnvironmentNaresuan UniversityPhitsanulok65000Thailand

This study was carried out to examine the effect of combining factors for immersion technique on masculinization of red tilapia(Oreochromis spp.).Three selected doses(1000,1,500,and 2000μg/L)of 17α-methyltestosterone(17 MT)immersion,2 exposure times(3 and 6 h)once or twice a week,and interaction of these factors were tested.A total of 12 treatment combinations under 3×2×2 factorial experiments in randomized complete block design were conducted to compare with the negative control group(no 17 MT-treated)and positive control group(17 MT-treated feed administration).One hundred red tilapia larvae(14 days post-hatch)were randomly allocated in each treatment and were operated in a 10-L glass jar for 21 *** the end of the treatment regime,the survival rate was ***,fish were raised in a 150-L glass tank using normal commercial feed for 2 months and sex ratios were determined via the gonad squash *** of the immersion treatments tested resulted in a statistically significant(p>0.05)decrease in survival *** 17 MT-treated groups produced progenies with a significantly higher(pmasculinization of red tilapia were *** male ratio was significantly affected by the dose-period and dose-frequency treatments(p<0.05).The highest male proportion(95.33%±0.58%)was recorded at 1000μg/L of 17 MT for 6 h once a week which was significantly higher(p<0.05)than other *** production cost of the best group was the cheapest(THB0.0066/fish)and decreased from the traditional procedure by about 10.81%.Therefore,this technique could be implemented for decreasing cost and operating process in commercial tilapia farms.

关键词： masculinization Red tilapia 17α-methyltestosterone Immersion technique

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The effectiveness of zinc supplementation in men with isolated hypogonadotropic hypogonadism

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Asian Journal of Andrology 2017年第3期19卷 280-285页

作者： Yan-Ling Liu Man-Na Zhang Guo-Yu Tong Shou-YueSun Yan-Hua Zhu Ying Cao Jie Zhang Hong Huang Ben Niu Hong Li Qing-Hua Guo Yan Gao Da-Long Zhu Xiao-Ying Li Shanghai Institute of Endocrinology and Metabolism Department of Endocrinology and Metabolism Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Endocrinology Drum Tower Hospital Affiliated to Nanjing University Medical School Nanjing 210008 China Department of Endocrinology The Third Affiliated Hospital Sun Yat-sen University Guangzhou 510630 China Department of Endocrinology and Metabolism Nanfang Hospital Southern Medical University Guangzhou 510515 China Department of Endocrinology Guangxi Medical University and First Affiliated Hospital Nanning 530021 China Department of Endocrinology The First People's Hospital of Yunnan Province Kunming 650032 China Department of Endocrinology The Affiliated Hospital of Guiyang Medical College Guiyang 550004 China 00853 China.

A multicenter, open-label, randomized, controlled superiority trial with 18 months of follow-up was conducted to investigate whether oral zinc supplementation could further promote spermatogenesis in males with isolated hypogonadotropic hypogonadism （IHH） receiving sequential purified urinary follicular-stimulating hormone/human chorionic gonadotropin （uFSH/hCG） replacement. Sixty-seven Chinese male IHH patients were recruited from the Departments of Endocrinology in eight tertiary hospitals and randomly allocated into the sequential uFSH/hCG group （Group A, n = 34） or the sequential uFSH plus zinc supplementation group （Group B, n = 33）. In Group A, patients received sequential uFSH （75 U, three times a week every other 3 months） and hCG （2000 U, twice a week） treatments. In Group B, patients received oral zinc supplementation （40 mg day-1） in addition to the sequential uFSH/hCG treatment given to patients in Group A. The primary outcome was the proportion of patients with a sperm concentration 〉1.0 × 106 ml-1 during the 18 months. The comparison of efficacy between Groups A and B was analyzed. Nineteen of 34 （55.9%） patients receiving sequential uFSH/hCG and 20 of 33 （60.6%） patients receiving sequential uFSH/hCG plus zinc supplementation achieved sperm concentrations ≥1.0 × 106 ml-1 by intention to treat analyses. No differences between Group A and Group B were observed as far as the efficacy of inducing spermatogenesis （P = 0.69）. We concluded that the sequential uFSH/hCG plus zinc supplementation regimen had a similar efficacy to the sequential uFSH/hCG treatment alone. The additional improvement of 40 mg day-1 oral zinc supplementation on spermatogenesis and masculinization in male IHH patients is very subtle.

关键词： gonadotropin isolated hypogonadotropic hypogonadism masculinization spermatogenesis zinc supplementation

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Analysis of the clinical characteristics and neonatal outcomes of seven cases of pregnancy luteoma discovered incidentally at cesarean section

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Gynecology and Obstetrics Clinical Medicine 2021年第2期1卷 83-86页

作者： Junhua Shen Lanlan Tang Genping Huang Xiaoxia Bai Baohua Li Zhengping Wang Department of Obstetrics Women’s HospitalZhejiang University School of MedicineXueshi Rd no.1HangzhouZhejiangPR China Department of Pathology Women’s HospitalZhejiang University School of MedicineXueshi Rd no.1HangzhouZhejiangPR China

Objective:In this study,we summarize the clinical characteristics and neonatal outcomes of patients with pregnancy luteomas that were identified incidentally at cesarean section in our *** also provide a review of the existing literature relating to this ***:A total of seven cases of pregnancy luteoma were enrolled from our hospital into this retrospective study between March 2013 and February *** then evaluated the clinical characteristics and neonatal outcomes of these ***:All seven patients with pregnancy luteomas found incidentally during Cesarean sections at term as a result of obstetric *** masses were unilateral and ranged from 2 to 10 cm in *** patients underwent partial ovariectomies or oophorocystectomy due to presumptive benign ovarian *** patients had gestational diabetes and one patient had gestational *** patient developed hirsutism and a deepened *** the seven newborns,three were girls and four were *** examinations of the newborns were normal and no virilization was detected among the infant ***:Pregnancy luteoma may be more common than *** strategies are now needed to educate obstetricians about the clinical features of pregnancy luteoma so that they can avoid unnecessary surgery.

关键词： Pregnancy luteoma Tumor-like ovarian lesion Ovarian tumor masculinization

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The factorial structure of self-reported androgen-promoted physiological traits

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Natural Science 2010年第10期2卷 1164-1170页

作者： Lee Ellis Shyamal Das 不详

Androgens make major contributions to average sex differences in anatomy, physiology, and behavior. Despite having established their crucial role in sexual differentiation, much remains to be learned about how androgens coordinate their influences. The present study was undertaken to shed light on androgenic effects on the body using self-reported survey data. We analyzed the ratings provided by over 11,000 college students on the magnitude of eleven traits that previous research has shown to be influenced by testosterone or other androgens. Predictably, the average values for all eleven traits were significantly greater in males than in females. Nevertheless, when data were analyzed separately according to sex of the respondents, some of the traits failed to positively correlate with one another, suggesting that not all an-drogen-influenced traits differentiate in a simple fashion. Factor analysis of these eleven traits by sex reinforced this view by identifying four factors. In men, the primary factor loaded most heavily on: masculine body build, masculine mannerisms, overall physical strength, upper body strength, and lower body strength. The primary factor for women was limited to: upper body strength, lower body strength, and overall physical strength. In both sexes, the primary factor was interpreted as reflecting the influence of perinatal and post-pubertal testosterone exposure. The other three factors may reflect the effects of other androgens (e.g., androstenediol), or the influence of female hormones such as estradiol. Findings were discussed in terms of future use of self-reported physiological measures for assessing androgenic effects on the human body.

关键词： Androgen-promoted physical traits Testosterone masculinization Physical strength Factorial structure Sex differences

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