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Journal of Integrative Plant Biology 2009年第3期51卷 279-286页

作者： Wen Li Lin Qi Xiaodong Lin Huhui Chen Zhanqi Ma Keqiang Wu Shangzhi Huang School of Life Sciences Sun Yat-sen University Guangzhou 510275 China Institute of Plant Biology School of Life Sciences National Taiwan University Taipei 106 China

A manganese superoxide dismutase （Mn-SOD） gene, NnMSD1, was identified from embryonic axes of the sacred lotus （Nelumbo nucifera Gaertn.）. The NnMSD1 protein contains all conserved residues of the Mn-SOD protein family, including four consensus metal binding domains and a signal peptide for mitochondrial targeting. Southern blot analysis suggests the existence of two *** genes in sacred lotus. NnMSD1 was highly expressed in developing embryonic axes during seed development, but appeared in cotyledons only at the early stage of development and became undetectable in the cotyledons during late embryogenesis. The expression of the NnMSD1 gene in germinating embryonic axes, in response to various stresses such as heat shock, chilling, and exposure to stress-related chemicals, was also studied. Heat shock strongly inhibited the expression of the NnMSD1 gene, whereas the NnMSD1 transcript level increased strongly in chilling stress treatment. An increase in expression was also highly induced by H2O2 in germinating embryonic axes. The results suggest that the expression pattern of the NnMSD1 gene differed between developing axes and cotyledons, and that the NnMSD1 gene expression responds strongly to chilling and oxidative stress.

关键词： manganese superoxide dismutase sacred lotus (Nelumbo nucifera Gaertn.) seed development and germination stress.

Mitochondrial DNA4977-bp deletion correlated with reactive oxygen species production and manganese superoxide dismutase expression in gastric tumor cells

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Chinese Medical Journal 2009年第4期122卷 431-436页

作者： WANG Juan LV You-yong Laboratory of Molecular Oncology Beijing Cancer Hospital andInstitute Peking University School of Oncology Beijing 100142China

Background Mitochondrial DNA 4977-bp deletion （△mtDNA^4977） was reported in many human neoplasia. However, its biological significance remains to be evaluated and the molecular mechanism needs to be investigated. In this study, we analyzed the frequency of △mtDNA^4977 in gastric cancer （GC） cell lines and tissues, as well as reactive oxygen species （ROS） contents and manganese superoxide dismutase （MnSOD） expression levels in GC cell lines to explore its biological significance and molecular mechanism. Methods Semi-quantitative PCR and real-time PCR were used to detect the incidence of △mtDNA^4977 in 13 GC cell lines and 272 human gastric tissues （108 GC specimens and the respective adjacent normal tissues, and 56 normal gastric mucosa from non-cancer patients）. We further identified intracellular ROS production by flow cytometry and MnSOD expression by semi-quantitative reverse transcription-PCR （RT-PCR） and Western blotting. Statistical analyses were carried out using the Logistic regression analysis and Kaplan-Meier method. Results Based on our earlier study, we optimized the PCR amplification condition by reducing the cycle number. In this study, we systematically documented the high incidence of △mtDNA^4977 in GC cell lines （10/13, 76.9%）, GC tissues （86/108, 79.6%）, matched normal tissues （73/108, 67.6%）, and normal gastric mucosa of non-cancer patients （29/56, 51.8%）. A significantly higher incidence of mutated △mtDNA^4977 was observed in GC tissues with respect to the adjacent normal tissues （79.6% vs 67.6%, P=-0.045）, and they were both higher than that in normal controls （P 〈0.05）. Most importantly, we linked the △mtDNA^4977 mutations with the expression level of MnSOD and ROS contents. The cell lines containing lower expression level of MnSOD was found to have generally higher frequent △mtDNA^4977 and more ROS. Conclusion The decreased anti-oxidative ability, which leads to increased ROS contents, is correlated with the mtDNA damage during gastric c

关键词： DNA, mitochondrial manganese superoxide dismutase reactive oxygen species stomach neoplasms

Differential Regulation of Proteins and a Possible Role for manganese superoxide dismutase in Bioluminescence of Panellus stipticus Revealed by Suppression Subtractive Hybridization

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Advances in Microbiology 2016年第9期6卷 613-626页

作者： Galina A. Vydryakova John Bissett ECORC Agriculture and Agri-Food Canada Ottawa Canada

Suppression subtractive hybridization (SSH) was employed to investigate bioluminescence in Panellus stipticus (Bull.) P. Karst. by detecting proteins differentially expressed in bioluminescent and luminescent strains. Comparisons of luminescent and non-luminescent monokaryon cultures of North American strains revealed differences in transcript levels of proteins responsible for post-translational modification (PTM) of enzymes. A similar comparison of a luminescent strain of P. stipticus from North America with a non-luminescent European strain revealed the presence of extracellular manganese superoxide dismutase (MnSOD) in the luminescent form, in addition to proteins involved in PTM. The application of MnSOD-specific inhibitors to luminescent mycelium resulted in the rapid loss of luminescence. The relevance to luminescence of proteins involved in PTM is discussed, together with a possible role for MnSOD that considers the potential for SODs to form stable complexes with catechols revealed in previously published research. In light of the recent discovery that hispidine may be the precursor of fungal luciferin, we consider a hypothetical mechanism for fungal luminescence in which the ο-hydroquinone moiety of a hispidine derivative ligates with the extracellular form of MnSOD producing a semiquinone-radical complex, with the resultant semiquinonato complex potentially reacting with molecular oxygen or other reactive oxygen species to produce sufficiently excited intermediates to emit light on relaxation.

关键词： Panellus stipticus Mushroom Bioluminescence Suppression Subtractive Hybridization manganese superoxide dismutase

Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer

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World Journal of Gastroenterology 2010年第37期16卷 4738-4746页

作者： Jian-Feng Yi Shi-Liang Kang Xiang-Ting Zeng Yu-Min Li Tao Liu Jun-Qiang Zhang Wen-Ting He Xun Li Wen-Ce Zhou The First College of Clinical Medicine of Lanzhou University Lanzhou 730000 Gansu Province China Key Laboratory of Digestive System Tumors Lanzhou University Second Hospital China Department of General Surgery The Fir st Hospital of Lanzhou Unive rsityLanzhou 730000Gansu ProvinceChina

AIM: To investigate the effects of superoxide dismutase (SOD) polymorphisms (rs4998557 , rs4880), Helicobacter pylori (H. pylori ) infection and environmental factors in gastric cancer (GC) and malignant potential of gastric precancerous lesions (GPL). METHODS: Copper-zinc superoxide dismutase (SOD1, CuZn-SOD)-G7958A (rs4998557 ) and manganese superoxide dismutase (SOD2, Mn-SOD)-Val16Ala (rs4880 ) polymorphisms were genotyped by SNaPshot multiplex polymerase chain reaction (PCR) in 145 patients with GPL (87 cases of gastric ulcer, 33 cases of gastric polyps and 25 cases of atrophic gastritis), 140 patients with GC and 147 healthy controls. H. pylori infection was detected by immunoblotting analysis. RESULTS: The SOD1-7958A allele was associated with a higher risk of gastric cancer [odds ratio (OR) = 3.01, 95% confidence intervals (95% CI): 1.83-4.95]. SOD216Ala/Val genotype was a risk factor for malignant potential of GPL (OR = 2.04, 95% CI: 1.19-3.49). SOD216Ala/genotype increased the risk of gastric cancer (OR = 2.85, 95% CI: 1.66-4.89). SOD1-7958A/genotype, SOD2-16Ala/genotype, alcohol drinking, positive family history and type Ⅰ H. pylori infection were associated with risk of gastric cancer, and there were additive interactions between the two genotypes and the other three risk factors. SOD2-16Ala/Val genotype and positive family history were associated with malignant potential of GPL and jointly contributed to a higher risk for malignant potential of GPL (OR = 7.71, 95% CI: 2.10-28.22). SOD1-7958A/genotype and SOD2-16Ala/genotype jointly contributed to a higher risk for gastric cancer (OR = 6.43, 95% CI: 3.20-12.91). CONCLUSION: SOD1-7958A/and SOD2-16Ala/-genotypes increase the risk of gastric cancer in Chinese Han population. SOD2-16Ala/-genotype is associated with malignant potential of GPL.

关键词： Copper-zinc superoxide dismutase manganese superoxide dismutase Gastric cancer Gastric precancerous lesions Gene polymorphisms Interaction

NADPH Oxidase Accounts for Changes in Cerebrovascular Redox Status in Hindlimb Unweighting Rats

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Biomedical and Environmental Sciences 2015年第11期28卷 799-807页

作者： PENG Liang RAN Hai Hong ZHANG Ying ZHAO Yu FAN Yong Yan PENG Li ZHANG Ran CAO Feng Department of Cardiology Chinese People's Liberation Army General Hospital Department of Geriatric Hematology Chinese People's Liberation Army General Hospital

Objective The roles of cerebrovascular oxidative stress in vascular functional remodeling have been described in hindlimb-unweighting （HU） rats. However, the underlying mechanism remains to be established. Methods We investigated the generation of vascular reactive oxygen species （ROS）, Nox2/Nox4 protein and mRNA levels, NADPH oxidase activity, and manganese superoxide dismutase （MnSOD） and glutathione peroxidase-1 （GPx-1） mRNA levels in cerebral and mesenteric smooth muscle cells （VSMCs） of HU rats. Results ROS production increased in cerebral but not in mesenteric VSMCs of HU rats compared with those in control rats. Nox2 and Nox4 protein and mRNA levels were increased significantly but MnSOD/GPx-1 mRNA levels decreased in HU rat cerebral arteries but not in mesenteric arteries. NADPH oxidases were activated significantly more in cerebral but not in mesenteric arteries of HU rats. NADPH oxidase inhibition with apocynin attenuated cerebrovascular ROS production and partially restored Nox2/Nox4 protein and mRNA levels, NADPH oxidase activity, and MnSOD/GPx-1 mRNA levels in cerebral VSMCs of HU rats. Conclusion These results suggest that vascular NADPH oxidases regulate cerebrovascular redox status and participate in vascular oxidative stress injury during simulated microgravity.

关键词： Microgravity Oxidative stress NADPH oxidase manganese superoxide dismutase Glutathione peroxidase-1

Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

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World Journal of Gastroenterology 2016年第17期22卷 4345-4353页

作者： Lei Li Hong-Hai Hong Shi-Ping Chen Cai-Qi Ma Han-Yan Liu Ya-Chao Yao Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical University Department of Biochemistry Zhongshan School of Medicine Sun Yat-sen University Department of Clinical Laboratory Medicine the Second People’s Hospital of Guangdong Province

AIM: To investigate the anti-apoptotic capability of the hepatitis B virus(HBV) in the HepG2 hepatoma cell line and the underlying ***: Cell viability and apoptosis were measured by MTT assay and flow cytometry, respectively. Targeted knockdown of manganese superoxide dismutase(Mn SOD), AMP-activated protein kinase(AMPK) and hepatitis B virus X protein(HBx) genes as well as AMPK agonist AICAR and antagonist compound C were employed to determine the correlations of expression of these ***: HBV markedly protected the hepatoma cells from growth suppression and cell death in the condition of serum deprivation. A decrease of superoxide anion production accompanied with an increase of Mn SOD expression and activity was found in Hep G2.215 cells. Moreover, AMPK activation contributed to the up-regulation of Mn SOD. HBx protein was identified to induce the expression of AMPK and Mn SOD. CONCLUSION: Our results suggest that HBV suppresses mitochondrial superoxide level and exerts an antiapoptotic effect by activating AMPK/Mn SOD signaling pathway, which may provide a novel pharmacological strategy to prevent HCC.

关键词： Hepatitis B virus Reactive oxygen species Apoptosis manganese superoxide dismutase AMP-activated protein kinase

Effect of Nitric Oxide on Esophageal Cancer Cell Line TE-1

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Chinese Medical Sciences Journal 2013年第1期28卷 44-49页

作者： Guo-gui Sun Wan-ning Hu Jun Zhang Cheng-lin Li Cong-rong Yang Department of Chemoradiotherapy Tangshan People's Hospital Affiliated to Hebei United University Department of Radiology Fourth Hospital of Hebei Medical University

Objective To investigate the radiosensitizing effect of nitric oxide(NO) combined with radiation on esophageal cancer cell line *** Methyl thiazolyl tetrazolium(MTT) assay was used to assess the effects of NO and radiation on TE-1 cells regarding inhibition of cell *** cytometry was used to examine the effect of NO and radiation on cell apoptosis and *** transcription polymerase chine reaction and Western blot were used to evaluete the effect of NO on mRNA and protein expression of manganese superoxide dismutase(MnSOD).Results NO inhibited the proliferation of TE-1 cells while significantly enhancing their *** application of NO combined with radiation significantly increased the apoptosis rate and G2/M phase proportion of TE-1 cells,with substantial decreases in the MnSOD mRNA and protein expression *** NO reduces the MnSOD mRNA and protein expression levels by affecting TE-1 cell cycle,further inhibiting the apoptosis of esophageal cancer cells and enhancing the killing effect of radiation on esophageal cancer cells.

关键词： nitric oxide manganese superoxide dismutase esophagealcancer cells radio-sensitization enhancement