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Levels of biological markers of nitric oxide in serum of patients with squamous cell carcinoma of the oral cavity

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International Journal of Oral Science 2013年第3期5卷 141-145页

作者： Wioletta Ratajczak-Wrona Ewa Jablonska Bozena Antonowicz Dorota Dziemianczyk Stanislawa Zyta Grabowska Department of Immunology Medical University of Bialystok Department of Oral Surgery Medical University of Bialystok Maxillofacial and Plastic Surgery Clinic Medical University of Bialystok

The aim of the study was a determination of the levels of nitric oxide（NO）and its biological markers such as malonyldialdehyde（MDA）and nitrotyrosine in the serum of patients with squamous cell carcinoma（SCC）of the oral cavity and identification of the relationships between NO and those *** studies were performed on patients with SCC of the oral cavity before and after *** reaction was used for the estimation of the total concentration of NO in *** nitrotyrosine level in serum was assessed with an enzyme-linked immunosorbent assay（ELISA）kit,and MDA level using a spectrophotometric *** concentrations of NO in blood serum were determined in patients with stage IV of the disease before treatment in comparison to the control group and patients with stages II and III of the ***,higher concentrations of MDA and nitrotyrosine were determined in the serum of patients in all stages of the disease in comparison to healthy *** treatment,lower concentrations of NO in the serum of patients with stage IV of the disease were observed in comparison to the amounts obtained prior to *** addition,lower levels of nitrotyrosine in the serum of patients with all stages of the disease were recorded,whereas higher concentrations of MDA were determined in these patients in comparison to results obtained before *** compounds formed with the contribution of NO,such as MDA and nitrotyrosine,may lead to cancer progression in patients with SCC of the oral cavity,and contribute to formation of resistance to therapy in these patients as ***,the lack of a relationship between concentrations of NO and MDA,and between NO and nitrotyrosine in serum suggests that the process of lipid peroxidation and nitration in patients with SCC does not just depend on NO.

关键词： malonyldialdehyde nitric oxide nitrotyrosine squamous cell carcinoma of the oral cavity

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Nitric oxide production is associated to increased lipoperoxidation and active caspase-3 in demyelinated brain regions of the taiep rat

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Advances in Bioscience and Biotechnology 2012年第6期3卷 695-704页

作者： Guadalupe Soto-Rodríguez Daniel Martínez-Fong Rosa Arroyo Patricia Aguilar-Alonso Hector Rubio José Ramón Eguibar Araceli Ugarte Maricela Torres-Soto Juan Antonio González-Barrios Jorge Cebada Eduardo Brambila Bertha Alicia Leon-Chavez Departamento de Fisiología Biofísica y Neurociencias Cinvestav Apdo. Postal 14-740 México D.F. 07000 México División de Medicina Genómica del Hospital regional 1o de Octubre ISSSTE Avenida Instituto Politécnico Nacional 1669 México D. F. 07760 México Escuela de Biología BUAP Puebla Pue. Mexico Facultad de Ciencias Químicas BUAP 14 sur y Av. Edif. 138 San Claudio 72570 Puebla Pue. México Facultad de Medicina Universidad Autónoma de Yucatán Mexico Instituto de Fisiología BUAP 14 sur 6301 Puebla Pue. México

We previously showed that the increase in nitric oxide (NO) levels and NO synthase (NOS) expression correlate with the progression of reactive astrocytosis and demyelination in the brains of 6-month-old taiep rats. Increased levels of NO can result in high concentration of peroxynitrite and thus cause tissue damage, which consists of lipoperoxidation of the cytoplasmic membrane, such as the myelin, and of apoptotic and necrotic cell-death. On this basis, we studied whether the increased NO production is associated with lipoperoxidation and cell death in the cerebellum and brainstem over the age (1, 3, 6, and 8 months) of taiep rats. The results were compared with those obtained in matched Sprague-Dawley (SD) rats. We measured the levels of nitrites (NO production), malonyldialdehyde, and 4-hydroxyalkenal (lipoperoxidation) in brain tissue homogenates. The three NOS isoforms and cleaved caspase-3 were evaluated by using ELISA and immunostaining techniques. Our results showed that NO production and lipoperoxidation increased in the cerebellum and brainstem as the age of the taiep rats increased compared to SD rats. The overexpression of nNOS and iNOS were in the Purkinje cells, magnocellular neurons, and in oligodendrocytes, whereas the glial cells showed strong cleaved-caspase-3 immunoreactivity. In summary our results suggest that NO plays a role in the demyelination and cell death in the taiep rat.

关键词： Hypomyelination Nitrite malonyldialdehyde Apoptosis Necrosis

A Clinical Study of Garlicin in Treating Acute Cerebral Infarction

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Chinese Journal of Integrative Medicine 1998年第2期4卷 89-94页

作者：史载祥张久亮毛建生张铁忠李海聪杜金行黄力曹启富自伯海石义亭刘爱华魏青

Objective: To evaluate the effect of garlicin in treating acute cerebral ***: One hundred and one patients of acute cerebral infarction diagnosed by CT were treated with garlicin (garlicin group) and compared with another group of 53 patients treated with ligustrazine (control group). The neurologic deficiency score and neurologic function score were used for evaluation of ***: The effective rates of garlicin and ligustrazine were 89. 36% and 84. 85% respectively. The improvement scores of neurologic function were 18.46±10.69 and 11.94 ± 6.79 respectively. The difference between the two groups was significant. Before treatment the serum level of MDA in the garlicin group was 6.015 ±1.26 nmol/ml, and it decreased to 4. 87 ± 1.16 nmol/ml after treatment, the change being more significant than that of the control group (from 6.02 ±0.73 nmol/ml to 6.47 ±1.04 nmol/ml). The rheological parameters and cerebral blood flow were also improved after garlicin ***: Garlicin could increase the perfusion flow of brain, improve the microcirculation, and scavenge free radicals so as to relieve the ischemia and reperfusion injury of the brain.

关键词： garlicin acute cerebral infarction ligustrazine malonyldialdehyde hemorheology

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PROTECTIVE EFFECT OF SHENG MAI SAN ON ADRIAMYCIN-INDUCED CARDIOTOXICITY——AN EXPERIMENTAL STUDY

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Medical Bulletin of Shanghai Jiaotong University 1989年第Z1期12卷 39-43页

作者：荣烨之温文虎傅良雄韩玉昇 Department of Internal Medicine Xinhua Hospital SSMU Shanghai Department of Biophysics Faculty of Basic Medical Sciences SSMU Shanghai

By measuring the contents of lipid-bound sialic acid (LSA), malonyldialdehyde (MDA), activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD) of the myocardial cells, it has been shown that Sheng Mai San can protect the myocardial cells from the adriamycin-induced cardiotoxicity ultrastructrally, inhibit their metabolism of lipid-bound sialic acid and improve the scavenging ability of myocardial cells for semiquinoid free radicals.

关键词： adriamycin lipid-bound sialic acid malonyldialdehyde glutathione peroxidase animal experiment superoxide dismustase

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