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Enhanced proliferation,invasion,and epithelial-mesenchymal transition of nicotine-promoted gastric cancer by periostin

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World Journal of Gastroenterology 2011年第21期17卷 2674-2680页

作者： Yu Liu Bao-An Liu Division of Basic Medicine Department ofPathology Central South University Xiangya School of Medicine Changsha 410013 Hunan Province China

AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were treated with nicotine and periostin protein expression was determined by *** mRNA in gastric cancer cells was silenced using small interfering RNA(siRNA) techniques and periostin gene expression was evaluated by quantitative reverse transcription-polymerase chain *** cancer cells transfected with control or periostin siRNA plasmid were compared in terms of cell proliferation using the methylthiazolyldiphenyl-tetrazolium bromide *** apoptosis was compared using annexin V-fluoresceine isothiocyanate and propidium iodine double *** invasion was determined using the Boyden chamber invasion assay,and the EMT marker Snail expression was evaluated by ***:Nicotine upregulated periostin in gastric cancer cells through a COX-2 dependent pathway,which was blocked by the COX-2-specific inhibitor *** mRNA expression was decreased by ～87.2% by siRNA in gastric cancer cells,and stable periostinsilenced cells were obtained by G418 ***-silenced gastric cancer cells exhibited reduced cell proliferation,elevated sensitivity to chemotherapy with 5-fluorouracil,and decreased cell invasion and Snail expression(P growth,invasion,drug resistance,and EMT facilitated by nicotine.

关键词： Cyclooxygenase-2 malignant growth RNA interference Snail Smoking

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