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Construction of dominant nuclear male sterility recurrent selection population in rice and its screening of salt tolerance plants

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Construction of dominant nuclear male sterility recurrent se...

Differential Expression Analysis of male sterility Induced by Space Flight with cDNA-AFLP in Maize

第十四届全国植物基因组学大会

作者： Yunlong Pang Jianlong Xu Zhikang Li Institute of Crop Science Chinese Academy of Agricultural Science

Recurrent selection is based on a population which has very abundant genetic basis, through several cycle of "selection—crossing-selection",breaking the genetic linkage between favorable and unfavorable genes,pyramid... 详细信息

关键词： male sterility recurrent selection introgression Lines salt tolerance

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Differential Expression Analysis of Male Sterility Induced b...

Genetic Study on Barley male sterility

第二届植物分子育种国际会议

作者： M.J. Cao1,2* L.B. Zhang1 G.T. Pan1 H. Lan1 Q.L. Tang1 Y.G. Zhu2 T.Z. Rong1 1 Maize Research Institute/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, 625014, China 2 Key Laboratory of Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan

＜正＞The cDNA-AFLP technique was used to analyze gene differential expression during the pollen development period of maize male sterility induced by space flight. Differential cDNA fragments were eluted, cloned,

关键词： Maize male sterility Space flight cDNA-AFLP

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Agricultural Science & Technology 2009年第4期10卷 108-111页

作者：张凤英刘志萍包海柱内蒙古农牧业科学院内蒙古呼和浩特010031 内蒙古农业大学农学院内蒙古呼和浩特010018

[Objective] The aim was to research the genetic mechanism of barley male sterility, and provide the theoretical reference for breeding strong heterosis hybrid of barley. [ Method] Fertility segregation phenomenon, morphological characteristics and main agronomic characters of male sterility character of 4 kinds of barley male sterility materials 2001 - 17, 2001 - 37, 2001 - 84 and 2001 - 116 and their dedved lines were observed and researched.[ Result] Barley male sterility existed genetic phenomenon of single dominant nuclear gene caused by environmental stimulation, its sterility controlled by MS, the sterile genotype was MSms. There was stable nucleo-cytoplasmic inheritance on barley male sterility which was controlled by cytoplasmic mate sterile gene S and nuclear gene rr, and its genotype was S（rr）, this male sterility belonged to CMS type sterility. Temperature had no effect on fertility. [ Conclusion] There was stable nucleo-cytoplasmic inheritance on barley male sterility, this result played a positively promoting role in barley practical production.

关键词： Barley male sterility Genetic mechanism

Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering male sterility in Plant

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Agricultural Science & Technology 2008年第2期9卷 81-84,128页

作者：刘立科刘根齐侯宁刘春光陈建南张文会樊颖伦吴冰洁中国科学院遗传发育所北京100101 聊城大学生命科学学院山东聊城252059

[ Objective] In order to study the relation between the HSPTO gene and male sterility of plant further. [ Methods ] Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HSP70 antisense fragment to construct HSPTO antisense expression vector. The expression vector was identified by PCR experiment and enzyme digestion. [ Result] The sequence of coloned Osg6B promoter had 97% homology to the published sequence, and the cis-regulatory element in promoter area was integrated. HSP70 antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion. [ Conclusion] The construction of expression vector would lay solid foundation for utilization of genetic engineering male sterility of plant.

关键词： HSP70 Osg6B promoter Gene engineering male sterility

Inheritance of the Pale—Green Leaf Marker and sterility Trait in Photo—Thermo Sensitive Genic male Sterile Rice

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Agricultural Sciences in China 2003年第3期2卷 245-249页

作者： TIANZhen－tao XUEQing－zhong DepartmentofAgronomy CollegeofAgricultureandBiotechnologyZhejiangUniversityHangzhou310029P.R.China DepartmentofAgronomy CollegeofAgricultureandBiotechnologyZhejiangUniversityHangzhou310029P.R.Ch

P/TGMS (photo-thermo sensitive genie male sterility) lines with pale-green leaf color have been developed in japonica rice. The marker trait is used as an assistant selection in the production of the two-lines system hybrid rice for the improvement of F, seed purity. A joint inheritance study of both leaf color and male sterility is presented for P/TGMS line with pale-green leaf color. The segregation ratios for leaf color in the F2 populations of the three crosses showed 13 : 3 and 15 : 1 at early and late sowing stages (April 26 and June 23) respectively, implying that the leaf color is controlled by two genes with fertility gene as dominant. sterility level is higher in the early sowing stage than that in the late sowing. The inducement of male sterility is closely related to longer day-length and higher temperature at the developmental stages of young panicle. The genes to govern the leaf color and male fertility are inherited independently.

关键词： Rice Pale-green leaf male sterility Agronomic trait P/TGMS

MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

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Science China(Life Sciences) 2021年第9期64卷 1533-1545页

作者： Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 311401China Core Facility and Technical Service Center for SLSB School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Cereal&Oil Crop Institute Hebei Academy of Agricultural and Forestry SciencesShijiazhuang 050031China

male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food *** male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid ***,the gene responsible for the male-sterile phenotype has remained ***,we report the map-based cloning and characterization of the MS1 gene in ***1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through *** ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid *** identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding.

关键词： soybean male sterility ms1 kinesin cytokinesis hybrid breeding

Ubiquitinome Profiling Reveals the Landscape of Ubiquitination Regulation in Rice Young Panicles

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Genomics, Proteomics & Bioinformatics 2020年第3期18卷 305-320页

作者： Liya Zhu Han Cheng Guoqing Peng Shuansuo Wang Zhiguo Zhang Erdong Ni Xiangdong Fu Chuxiong Zhuang Zexian Liu Hai Zhou State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Instrumental Analysis and Research CenterKey Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhou 510060China School of Life Sciences Zhengzhou UniversityZhengzhou 450001China College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesNational Centre for Plant Gene ResearchBeijing 100101China

Ubiquitination,an essential post-transcriptional modification(PTM),plays a vital role in nearly every biological process,including development and *** its functions in plant reproductive development,its targets in rice panicles remain *** this study,we used proteome-wide profiling of lysine ubiquitination in rice(*** ***)young *** created the largest ubiquitinome dataset in rice to date,identifying 1638 lysine ubiquitination sites on916 unique *** detected three conserved ubiquitination motifs,noting that acidic glutamic acid(E)and aspartic acid(D)were most frequently present around ubiquitinated *** analysis of Gene Ontology(GO)annotations and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young ***,enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptorlike kinases and cytoplasmic tyrosine and serine-threonine ***,we analyzed the crosstalk between ubiquitination,acetylation,and succinylation,and constructed a potential protein interaction network within our rice ***,we identified ubiquitinated proteins related to pollen and grain development,indicating that ubiquitination may play a critical role in the physiological functions in young *** together,we reported the most comprehensive lysine ubiquitinome in rice so far,and used it to reveal the functional role of lysine ubiquitination in rice young panicles.

关键词： Lysine ubiquitination Ubiquitinome Rice Grain development male sterility

ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development

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Molecular Plant 2023年第8期16卷 1321-1338页

作者： Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei Tingwei Yan Yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu Yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-NanYang Xiangyuan Wan Research Institute of Biology and Agriculture University of Science and Technology BeijingBeijing 100083China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Zhongzhi International Institute of Agricultural BiosciencesBeijing 100192China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTaianShandong 271018China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China

Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific ***,the molecularmechanismunderlying exine formation and thickness remains *** this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in *** an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,*** thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of *** activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and ***,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation.

关键词： ZmMs1/ZmLBD30 LBD transcription factor feedback repression exine male sterility maize

Construction and Analysis of Suppression Subtractive cDNA Library from Fertile Disk Floret Buds of Zinnia elegans

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Journal of Integrative Agriculture 2012年第4期11卷 567-575页

作者： PANG Rui-hua MA Guang-ying LOU Xue-yuan BAO Man-zhu YE Yao-mei Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 P.R.China College of Life Sciences Xinyang Normal University Xinyang 464000 P.R.China

In order to isolate genes related to anther development and understand molecular basis of male sterility,cDNA library of Zinnia elegans was constructed using suppression subtractive hybridization（SSH） approach.672 different expressed clones were selected from the fertile disk floret *** results showed that cDNA inserts were ranged from 100 to 750 bp.303 positive clones screened by dot-blot hybridization were sequenced.273 out of 303 sequenced clones produced readable sequences;these sequences represent 87 non-repetitive *** homology alignment showed that 76 expressed sequence tags（ESTs） had functional annotations in GenBank,the other 11 ESTs without any homology to the known *** addition,87 ESTs were divided into 17 groups according to MIPS of Arabidopsis thaliana *** data from the cDNA library have been deposited with the GenBank under the accession numbers *** an important result in this study,7 genes related to anther development were *** from semi-quantitative RT-PCR showed 6 genes were expressed only in disk florets of fertile plants compared with that of male sterile *** ESTs obtained provide important clues for further isolation and identification of fertility-related genes in ***.

关键词： Zinnia elegans suppression subtractive hybridization(SSH) male sterility anther development expressed sequence tags(ESTs)