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Mosquito larvicidal and pupicidal activity of Euphorbia hirta Linn.(Family:Euphorbiaceae) and Bacillus sphaericus against Anopheles stephensi Liston.(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2013年第2期6卷 102-109页

作者： C.Panneerselvam K.Murugan K.Kovendan P.Mahesh Kumar J.Subramaniam DRDO-BU Center for Life Sciences Bharathiar UniversityCoimbatore-641046Tamil NaduIndia Division of Entomology Department of ZoologySchool of Life Sciences.Bharathiar UniversityCoimbatore-641046Tamil NaduIndia

Objective:To explore the larvicidal and pupicidal activity of Euphorbia hirta（***） leaf extract and Bacillus sphaericus（***） against the malarial *** siephensi（An. stephensi）.Methods:The larvicidal and pupicidal activity was assayed against *** at various concentrations ranging from（75-375 ppm） under the laboratory as well as field conditions. The LC50 and LC90 value of the *** leaf extract was determined by probit ***: The plant extract showed larvicidal effects after 24 h of exposure;however,the highest larval mortality was found in the methanol extract of *** against the first to fourth instars larvae and pupae of values LC50=137.40,172.65,217.81,269.37 and 332.39 ppm;*** against the first to fourth instars larvae and pupae of values LC50= 44.29,55.83,68.51,82.19 and 95.55 ppm, ***,combined treatment of values of LC50= 79.13,80.42,86.01,93.00 and 98.12 ppm,*** mortality was observed in the ***:These results suggest methanol leaf extracts of *** and *** have potential to be used as an ideal ecofriendly approach for the control of the malarial *** as target species of vector control *** study provides the first report on the combined mosquito larvicidal and pupicidal activity of this plant crude extract and bacterial toxin against *** mosquitoes.

关键词： Euphorbia hirta Anopheles stephensi Bacillus sphaericus Larvicidal and pupicidal activity Field trial malarial vector

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The　development　of　a　recombinant　hybrid　protein　of　Plasmodium　falciparum　and　analysis　of　its　antigenicity　and　protectivity

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Journal of Medical Colleges of PLA(China) 1994年第3期9卷 161-169页

作者：李全贞李英杰谢毅任大明毕惠祥徐秉锟

A hybrid gene encoding several putative protective epitopes from erythrocytic stage antigens (MSA1,MSA2 and RESA) of P. falciparum as well as exgenous T cell enhancer epitopes from interleukin-1 and tetanus toxin was synthesized chemically. This gene (named HGFC) was cloned and connected with another hybrid gene (HPFA) synthesized previously to make a bigger hybrid gene (HGFCAC). HGFC and HGFCAC were cloned in an expression vector pWR450-1 and transformed into E. Coli JM109. The engineered bacteria could express fusion proteins with molecular weights of 65 and 77 kDa after inducing with isopropylthio-β-D-galactoside (IPTG). The expression rate was about 35% of total bacterial proteins. The expressed products showed sepcific immunological reaction with rabbit antibodies against P. falciparum peptide Glu-Glu-Asn-Val-Glu-His-Asp-Ala (EENVEHDA)by Western blotting. The fusion proteins were pruified by precipitation with amonium sulfate. gel filtration and ion-exchange chromatography and the purity was 82%. The purified protein reacted specifically with mouse immune serum against falciparum blood stage antigens by dot enzyme-linked immunosorbent assay (dot-ELISA).The fusion protein was emulsified with Freund's complete adjuvant (FCA) and used to immunize rabbits. The immune serum can recognize P. falciparum erythrocytic stage antigens of Fcc-1/HN strain and Yunnan strain and had weak cross reaction with P. vivax,but had no reaction with P. cynomlogi and P. berghei antigens. The protective effect of the antibody was tested by in vitro inhibition test to cultured falciparum parasites. Preliminary results indicated that the immune sera could effectively reduce the invasion rates of the parasites to red blood cells and inhibit the growth of the in vitro cultured falciparum parasites. The inhibitory capacity of the immune sera to parasite invasion is enhanced as the amount of the sera increases and the incubation time of the sera with the parasites is *** was shown th

关键词： Plasmodium falciparum recombinant protein hybrid antigen inhibition test in vitro malarial vaccine

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Subcloning　and　expression　of　two　conserved　regions　（Ⅰ，Ⅴ）　onP190　antigen　of　Plasmodium　falciparum　in　***

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Journal of Medical Colleges of PLA(China) 1994年第1期9卷 36-39页

作者：潘卫庆杨树桐邓海琳陆德如

Two DNA fragments, designated as P190CRI (AA1-55) and P190CRV (AA1597-1667)respectively, which encode amino acid residues of conserved region Ⅰ and Ⅴ on the P190 antigen were amplified by polymerase chain reaction from genomic DNA in FCCl/HN strain of Plasmodium falciparum isolated from Hainan Province, China. It was found that there were five base substitutions in the P190CR V, in comparison with the nucleotide sequences of MAD20 strain. These two fragments sequenced were inserted into pGEX-2T plasmid. E. coli JM109 (DE3) were transformed with the recombinant plasmids and the parental plasmid. The results showed that the two fragments were expressed as high-level C-terminal fusions with glutathione S-transferase (GST). The fusion proteins were easily purified from bacterial lysates by affinity chromatography using glutathione Sepharose 4B.

关键词： P190 antigen Plasmodium falciparum malarial vaccine genetic engineering

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The Analysis of Results of Surveillance on Malaria for the Chinese Workers Who Worked in Gabon

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旅行医学科学 1995年第4期1卷 188-189页

作者： Du Jie Liu Xiaomei Wang Yuping Dalian Health Quarantine Bureau, P. R. China

The retrospect investigation for 127 returned Chinese labor services who worked in Gabon were carried out, and malaria blood smear, blood serum test were carried out. There were 32 workers got malaria, the proportion was 25. 2 per cent. For three of the patients, the malarial parasite test presented positive reaction, and the superintendence of quarantine doctor, they were isolated to cure. During the three patients had another suffering from malaria fever,they took antimalaria medicine, their symptom was improved, the course of disease was reduced, and the curative affect was obvious. For the Chinese labor services who go to the serious malaria country, it is important that the health protection of travel medicine should be done well.

关键词： malaria malarial parasite retrospect investigation

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