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Identification of KCS gene family and functional analysis of FAE-like genes from malania oleifera

Oil Crop Science 2021年第1期6卷 35-40页

作者： Huan Dai Yanyu Zhang Pei Jin Fengping Song Wei Xu Aizhong Liu Zuqing Meng Tianquan Yang Department of Plant Science Tibet Agriculture and Animal Husbandy CollegeLinzhi860000China College of Life Science Northwest UniversityXi’an710069ShaanxiChina Southwest Forestry University Kunming650224China Kunming Institute of Botany Chinese Academy of SciencesKunming650201China

KCS(3-ketoacyl-CoA synthase)is the key enzyme catalyzing the first step of very long chain fatty acid(VLCFA)biosynthesis.Studies showed that different KCSs possessed different substrate preference.malania oleifera are abundance of VLCFAs in its mature seeds,especially the nervonic acid,which is essential for human health.In this study,we identified and characterized 18 KCS genes in M.oleifera genome.Phylogenetic analysis showed that these KCS genes were classified into four subfamilies,including two FAE-like,six KCS-like,eight FDH-like and two CER6.We concentrated on the functional role of two FAE-like genes,Maole003085.T1 and Maole004215.T1 which encoded predicted amino acid residues of 516 and 518 in protein,respectively.Multiple sequence alignment showed that their two proteins contained the known and conserved active sites among FAE-like subfamily.Upon heterologous expression in wild type yeast(Saccharomyces cerevisiae)INVSc1,we found that Maole004215.T1 could produce four new fatty acids including C22:0/C22:1 and C24:0/C24:1,but Maole003085.T1 only produced C22:1.Besides,upon heterologous expression in mutant yeast BY4741-△elo3,we found the Maole003085.T1 could produce C24:0 and C26:0,while the Maole004215.T1 could catalyze the formation of fatty acids C24:0,C26:0 and C28:0.These results showed Maole003085.T1 and Maole004215.T1 had fatty acid elongation activity in yeast,and possessed different substrate preference in the production of different VLCFAs.Interestingly,we found Maole004215.T1 could produce nervonic acid in yeast,which provides molecular basis on the genetic improvement and genic engineering for producing nervonic acid resources by using biotechnological methods.

关键词： malania oleifera KCS gene Very long chain fatty acid Nervonic acid

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Germplasm resources of three wood plant species enriched with nervonic acid

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Plant Diversity 2022年第3期44卷 308-315页

作者： Xing He Tian-Quan Lu Jiang-Ying Li Ping Mao Li Zhang Guo-Wei Zheng Bo Tian Key Laboratory of Sustainable Utilization of Tropical Plant Resources Xishuangbanna Tropical Botanical GardenChinese Academy of Sciences88 Xuefu RoadKunming650223China University of Chinese Academy of Sciences Beijing100049China Xinping Branch Yuxi Tobacco CompanyXinping653400China d Guangnan Forestry and Grassland Bureau Guangnan663300China Traditional Chinese Medicine College Yunnan University of Chinese MedicineKunming650500China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan666303China

Nervonic acid(NA)is a very-long-chain monounsaturated fatty acid with pharmaceutical and nutraceutical functions that plays an important role in treating several neurological disorders.One major source of NA is plant seed oil.Here we report fatty acid profiles of seeds and germplasm diversity of six plant species,including three woody plants with high amounts of NA-enriched seed oil,malania oleifera,Macaranga adenantha,and M.indica.M.oleifera had the largest seed(average 7.40 g single seed),highest oil content(58.71%),and highest NA level(42.22%).The germplasm diversity of M.oleifera is associated with its habitat but not elevation.Seeds of M.adenantha contained higher NA levels(28.41%)than M.indica(21.77%),but M.indica contained a significantly higher oil content(29.22%)and seed yield.M.adenantha germplasm varied among populations,with one population having seeds with high oil content(22.63%)and NA level(37.78%).Although M.indica grow naturally at a range of elevations,no significant differences were detected between M.indica populations.These results suggest that M.indica and M.oleifera have greater potential as a source of NA,which will contribute to constructing a germplasm resource nursery and establishing a selection and breeding program to improve the development of NA-enriched plants.

关键词： Nervonic acid Germplasm resource malania oleifera Macaranga adenantha Macaranga indica Diversity

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Lipid analysis of three special nervonic acid resources in China

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Oil Crop Science 2020年第4期5卷 180-186页

作者： Xinghao Tu Jingya Wan Ya Xie Fang Wei Siewyoung Quek Xin Lv Liqing Du Hong Chen Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Oilseeds Processing of Ministry of Agriculture and Rural AffairsKey Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural AffairsHubei Key Laboratory of Lipid Chemistry and NutritionWuhanChina South Subtropical Crop Research Institute Chinese Academy of Tropical Agricultural Science/Key Laboratory of Tropical Fruit BiologyMinistry of Agriculture and Rural AffairsZhanjiangChina Faculty of Life Science Hubei UniversityWuhanChina School of Chemical Sciences University of AucklandAuckland1142New Zealand

Nervonic acid plays an important role in nutrition and function of the human body.malania oleifera,Acer truncatum and Xanthoceras sorbifolium are China’s unique woody plant rich in nervonic acid in seed oil.This study aims to investigate the lipid composition of these 3 special resources.Their fatty acids were detected by gas chromatography coupled with flame detector(GC-FID).Triglycerides(TAGs)and phospholipids(PLs)were detected by shotgun-mass spectrometry(shotgun-MS).Results showed that M.oleifera oil presented the highest level of nervonic acid(46.20±0.22%)among the 3 oils.Seeds oil of A.truncatum and X.sorbifolium had 3.53±0.20%and 1.83±0.21%nervonic acid respectively.53 species of TAGs and 15 species of PLs were identified in M.oleifera oil,with PLs content of 499.94±22.34μg/g.In A.truncatum oil,PL and TAG species were twice more than those in M.oleifera oil,and its’content of PLs was 76.27±3.21μg/g.In X.sorbifolium oil,75 TAGs and 34 PLs were detected,with the lowest PLs at 23.84±0.17μg/g.The results demonstrated that these 3 vegetable oils have great potential to become nervonic acid supplements for human health.

关键词： Nervonic acid malania oleifera Acer truncatum Xanthoceras sorbifolium Lipid analysis

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