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magnetic labeling of natural lipid encapsulations wit ron-based nanoparticles

Nano Research 2018年第6期11卷 2970-2991页

作者： Dewen Ye Yan Li Ning Gu State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Sciences & Medical Engineering Southeast University Nanjing 210096 China Collaborative Innovation Center of Suzhou Nano Science and Technology Suzhou 215123 China

With superior biocompatibility and unique magnetic properties, iron-based nanoparticles （IBNP） are commonly encapsulated in cells and extracellular vesicles （EV） to allow for magnetic force controlled drug delivery and non-invasive tracking. Based on their natural source and similar morphologs; we classify both cells and EVs as being natural lipid encapsulations （NLEs）, distinguishing them from synthetic liposomes. Both their imaging contrast and drug effects are dominated by the amount of iron encapsulated in each NLE, demonstrating the importance of magnetic labeling efficiency. It is known that the membranes function as barriers to ensure that substances pass in and out in an orderly manner. The most important issue in increasing the cellular uptake of IBNPs is the interaction between the NLE membrane and IBNPs, which has been found to be affected by properties of the IBNPs as well as NLE heterogeneity. Two aspects are important for effective magnetic labelling： First, how to effectively drive membrane wrapping of the nanoparticles into the NLEs, and second, how to balance biosafety and nanoparticle uptake. In this review, we will provide a systematic overview of the magnetic labeling of NLEs with IBNPs. This article provides a summary of the applications of magnetically labeled NLEs and the labeling methods used for IBNPs. The review also analyzes the role of IBNPs physicochemical properties, especially their magnetic properties, and the of NLEs in the internalization pathway. At the same time, the future of magnetically labeled NLEs is also discussed. development

关键词： magnetic labeling iron-based nanopartide （IBNP） natural lipid encapsulation（NLE） IBNP-membrane interactions

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Highly Efficient labeling of Human Lung Cancer Cells Using Cationic Poly-L-lysine-Assisted magnetic Iron Oxide Nanoparticles

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Nano-Micro Letters 2015年第4期7卷 374-384页

作者： Xueqin Wang Huiru Zhang Hongjuan Jing Liuqing Cui College of Bioengineering Henan University of Technology

Cell labeling with magnetic iron oxide nanoparticles(IONPs)is increasingly a routine approach in the cellbased cancer treatment.However,cell labeling with magnetic IONPs and their leading effects on the biological properties of human lung carcinoma cells remain scarcely reported.Therefore,in the present study the magnetic c-Fe2O3nanoparticles(MNPs)were firstly synthesized and surface-modified with cationic poly-L-lysine(PLL)to construct the PLL-MNPs,which were then used to magnetically label human A549 lung cancer cells.Cell viability and proliferation were evaluated with propidium iodide/fluorescein diacetate double staining and standard 3-(4,5-dimethylthiazol-2-diphenyl-tetrazolium)bromide assay,and the cytoskeleton was immunocytochemically stained.The cell cycle of the PLL-MNPlabeled A549 lung cancer cells was analyzed using flow cytometry.Apoptotic cells were fluorescently analyzed with nuclear-specific staining after the PLL-MNP labeling.The results showed that the constructed PLL-MNPs efficiently magnetically labeled A549 lung cancer cells and that,at low concentrations,labeling did not affect cellular viability,proliferation capability,cell cycle,and apoptosis.Furthermore,the cytoskeleton in the treated cells was detected intact in comparison with the untreated counterparts.However,the results also showed that at high concentration(400 lg m L-1),the PLL-MNPs would slightly impair cell viability,proliferation,cell cycle,and apoptosis and disrupt the cytoskeleton in the treated A549 lung cancer cells.Therefore,the present results indicated that the PLL-MNPs at adequate concentrations can be efficiently used for labeling A549 lung cancer cells and could be considered as a feasible approach for magnetic targeted anti-cancer drug/gene delivery,targeted diagnosis,and therapy in lung cancer treatment.

关键词： magnetic labeling Iron oxide nanoparticles Poly-L-lysine Human A549 lung cancer cells Cancer treatment

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