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An active piggyBac-like element in macdunnoughia crassisigna

Insect Science 2008年第6期15卷 521-528页

作者： Min Wu Zhi-Chan Sun Chun-Lin Hu Gu-Feng Zhang Zhao-Jun Han Key Laboratory of Monitoring and Management of Plant Disease and Insects Ministry of Agriculture Nanjing Agricultural University Nanjing Tongzhou Station of Plant Protection Tongzhou Jiangsu Province China

In this paper, a highly conservedpiggyBac-like sequence, designated as McrPLE was cloned from a lepidopteran insect, macdunnoughia crassisigna. It is 2 472 bp long in full length with a single open reading frame and encodes a 595 amino acid transposase. It shares identical terminal and sub-terminal repeats with T. ni IFP2 and is flanked by the typical TTAA target-site duplications. Alignment and phylogenetic analysis revealed that McrPLE had greater than 99.5% identity and appeared to be the closest one in phylogeny to IFP2 among the PLEs so far found in various species. Plasmid-based excision and transposition assay proved it was mobile in cell culture. Otherwise, McrPLE element and all other highly conserved IFP2 sequences reported previously were found to share three common nucleotide substitutions. This suggests that the original IFP2 may be a related variant of a predecessor element that became widespread. The existence of nearly identical piggyBac sequence in reproductively isolated species was thought also a strong indication of horizontal transmission, which raises important considerations for the stability and practical use ofpiggyBac transformation vectors.

关键词： piggyBac-like element (PLE) macdunnoughia crassisigna transposon

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