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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Yue-mei Feng~(1,3),Ling-Yan Su~(1,3),De-Xiang Li~2,Yi-Ping Zhang~2,Yong-Gang Yao~1 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province,Kunming Institute of Zoology,Kunming,Yunnan 650223,China 2.Yunnan Police Officer Academy,Kunming,Yunnan 650223,China 3.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China

Drug addiction is a very serious social problem and continues to cause enormous financial costs on society. The available treatments remain to be inadequate for the drug abusers and addiction relapse rates vary depending on the substance being ***,an improved understanding of the biological change during the process of drug abstinence will lead to more effective treatment and eventually to improve the recovery of drug *** studies have demonstrated that the endocrine and metabolism systems were actively involved in this *** is the main secretory product of the pineal gland which is known to affect drug *** also functions as a powerful antioxidant to protect mitochondria and mtdna from the oxidative damage caused by reactive oxygen species in the central nervous *** hypothesized that the change of plasma melatonin level and alteration of mtdna in peripheral blood could be used as biomarkers for better drug *** this study,we detected the plasma melatonin level and mtdna copy number change and damage in 13 opiate dependent females who received drug detoxification at 0,3 and 6 *** overall pattern in the drug abusers during abstinence was compared to that of 17 matched normal *** opiate abusers had a mean age of 31.7±2.4 years and were recruited from the Kunming Compulsory Drug Detoxification *** control group had a mean age of 30±1.7 years and had no history of medical,neurological or psychiatric *** melatonin level was quantified by enzyme-linked immunosorbent assay(ELISA).Quantitative real-time PCR was used to detect mtdna copy number change and *** melatonin level,mtdna copy number and mtdna damage between the normal controls and drug abusers undergoing abstinence at different time points were compared by unpaired t *** also examined the time-dependent change of plasma melatonin level, mtdna copy number change and damage in these drug abusers during drug abst

关键词： drug addiction melatonin mtdna copy number mtdna damage

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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero Estel Vinolas‑Vergés Sergi Bonet Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Institution for Research and Advanced Studies(ICREA) Barcelona ES‑08010Spain

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtdnac) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtdnac and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtdnac and sperm function and fertilising potential in pigs. A qPCR method for mtdnac quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently *** First, the q PCR method was found to be sensitive and efficient for mtdnac quantification in pig sperm. By using this technique, mtdnac was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtdnac were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater *** These findings enrich our comprehension of the association of mtdnac with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

关键词： Fertility Mitochondria Motility mtdna copy number qPCR Sperm

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