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morn motifs in plant PIPKs are involved in the regulation of subcellular localization and phospholipid binding

Cell Research 2006年第5期16卷 466-478页

作者： Hui Ma~(1,*) Ying Lou~1 Wen Hui Lin~1 Hong Wei Xue~1 ~1National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Science(SIBS),Chinese Academy of Sciences and Parmer Group of Max-Planck-Institute of Molecular Plant Physiology(MPI-MP)on"Plant Molecular Physiology and Signal Transduction",300 Fenglin Road,200032 Shanghai,China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and Signal Transduction” Shanghai China Unit of Molecular Signal Transduction ERRB NICHD Bldg 49 Bethesda

Multiple repeats of membrane occupation and recognition nexus （morn） motifs were detected in plant phosphati-dylinositl monophosphate kinase （PIPK）,a key enzyme in PI-signaling *** analysis indicates that allthe morn motifs （with varied numbers at ranges of 7-9）,which shared high homologies to those of animal ones,werelocated at N-terminus and sequentially arranged,except those of OsPIPK1 and AtPIPK7,in which the last morn motifwas separated others by an～100 amino-acid"island"region,revealing the presence of two kinds of morn arrangementsin plant *** employing a yeast-based SMET （sequence of membrane-targeting） system,the morn motifswere shown being able to target the fusion proteins to cell plasma membrane,which were further confirmed by expressionof fused morn-GFP *** detailed analysis via deletion studies indicated the morn motifs in OsPIPK1,together with the 104 amino-acid"island"region are involved in the regulation of differential subcellular localization,*** membrane or nucleus,of the fused *** Western blot analysis of the recombinant morn polypep-tide,expressed in Escherichia coli,showed that morn motifs could strongly bind to PA and relatively slightly to PI4Pand PI（4,5）*** results provide informative hints on mechanisms of subcellular localization,as well as regulationof substrate binding,of plant PIPKs.

关键词： membrane targeting PIPK morn motif subcellular localization

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