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Sex Control of Silkworm Bombyx mori by UsingSensitive Character to IncubatingTemperature and Humidity

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Chinese Science Bulletin 1994年第1期39卷 67-71页

作者：潘庆中陈业林陈劲伟林健荣黄自然 Department of Sericulture South China Agricultural University Guangzhou 510642 PRC Regional Sericulture Training Center for Asia-Pacific

Because the male silkworm is economically superior to the female in sericulture,male-silkworm rearing through sex control is an interesting question. In order to rearmale silkworms only, a balanced lethal system was established in Japan and former *** utilizing the recessive female sex-linked gene. Tajima et al. eliminated femaleindividuals by means of mechanical or manual methods according to the sex-limitedegg color or sex-limited larva marking. In addition, Xu An-ying et al. tried to ob-tain only male individuals by inducing androgenesis. However, the above methodscannot yet be utilized in sericulture production because of their harmful influenceson the vitality of silkworm larvas or the hatching rate.

关键词： silkworm Bombyx mori sex control incubation.

Expression and subcellular localization of a novel gene Bm-Xof the silkworm, Bombyx mori

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American Journal of Molecular Biology 2012年第3期2卷 224-231页

作者： Zhengbing Lv Yunlong Liu Ying Chen Bo Li Lili Liu Jian Chen Zuoming Nie Qing Sheng Wei Yu Yaozhou Zhang Institute of Biochemistry Zhejiang Sci-Tech University Hangzhou China

According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained its ORF for further study by bioinformatics analysis. It is 444 bp and encodes 147 amino acid residues, with a predicted molecular weight (MW) of 16.4 kD and an isoelectric point (pI) of 3.69. In this study, we successfully constructed a recombinant plasmid pET-28a(+)-Bm-X and expressed it in Escherichia coli. We used the fusion protein rBm-X which purified by Niaffinity chromatography to produce polyclonal antibodies against Bm-X for Western blot analysis. The analysis revealed that Bm-X was expressed in the larval midgut, the epidermis and the silk gland. In addition, the subcellular localization analysis of silkworm ovary epithelial cells (BmN cells) showed that Bm-X protein was located both in cytoplasm and nucleus, and the signal was stronger in cytoplasm than in nucleus. Our findings indicate that Bm-X gene is a novel species-specificity gene and its expression product can be detected in tissues of the fifth silkworm instar larvae and BmN cells.

关键词： Bombyx mori Novel Gene Expression Subcellular Localization Bm-X

Critical analysis of correlation and heritability phenomenon in the silkworm, Bombyx mori(Lepidoptera: Bombycidae)

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Advances in Bioscience and Biotechnology 2011年第5期2卷 347-353页

作者： Tribhuwan Singh Madan Mohan Bhat Mohammad Ashraf Khan 不详

Correlation and heritability studies of quantitative traits are a pre-requisite for judicious selection for genetic improvement of complex characters of economic importance. The success of selection is governed by the degree to which the desired trait is transmitted to the succeeding generation. The nature of selection is to be given due consideration at appropriate developmental stages for pursuing selection in desired direction while improving or evolving high productive breeds or hybrids of the silkworm. Characters showing high heritability as well as high genetic advance respond better to simple phenotypic selection while those having low heritability and low genetic advance may respond better to mass selection. Characters showing high heritability and low genetic advance may yield good response to hybridization and recurrent selection. An attempt has been made in this review article to briefly discuss the magnitude of correlation and heritability in selection strategies for the improvement of quantitative traits in desired direction in the silkworm, Bombyx mori (L.).

关键词： Bombyx mori Correlation Heritability

Analysis of ESTs and gene expression patterns of the posterior silkgland in the fifth instar larvae of silkworm, Bombyx mori L.

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Science China(Life Sciences) 2005年第1期48卷 25-33页

作者： ZHONG Boxiong, YU Yingpo, XU Yusong, YU Hong, LU Xingmeng, MIAO Yungen, YANG Jun, XU Hao, HU Songnian & LOU Chengfu College of Animal Sciences, Zhejiang University, Hangzhou 310029, China Hangzhou Huada Gene Research and Development Center, Southern Base of Genomic Information, Chinese Academy of Sciences, Hangzhou 310007, China College of Animal Sciences Zhejiang University Hangzhou310029 China Hangzhou Huada Gene Research and Development Center Southern Base of Genomic Information Chinese Academy of Sciences Hangzhou310007 China

The fibroin gene expression pattern and regulation of the posterior silkgland were studied by means of expressed sequence tags (ESTs) using the first and fifth day larvae of the fifth instar of silkworm, Bombyx mori L (strain: C 108). The results showed that there were 911 repetitive ESTs and 1950 single sequences (Singlets) among total 2861 consentient sequences, which were spliced. 1335 sequences were identified and the other 1526 were unknown. 5560 sequences (55.89%) in the posterior silkgland cell of the silkworm were new ESTs without homology with EST data published by Mita et al. The number of repetitive ESTs and single sequences from the first day larvae of the fifth instar was double more than that of the fifth day of the same instar in the silkworms. The unigenes which were more than 50 in repetitive EST size (contig size) came to only about 0.5% in total consentient sequences. There were significant differences between gene expression frequencies, and expressed genes were related to fibroin synthesis and its secretion and fibroin composition. Comparing the fifth day with the first day of the fifth instar, the genes-expressed quantity of fibroin heavy-chain gene was 18 fold higher, fibroin light-chain gene 9 fold and fibroin P52 gene 8 fold. 508 genes functioned for cellular component and 315 for enzyme after function tracing. These results implied that the gene expression of the first day was mainly for preparation for fibroin synthesis except for the growth of silkgland cells, and the gene expression of the fifth day of the fifth instar was mainly for synthesizing and excreting fibroin. Because the ratio of heavy chain, light chain and p25 of fibroin was not 6:6:1 as theoretically expected, or its special H-chain structure, the H-chain gene was not easy to detect through EST technique. Most of genes among total 2861 consentient sequences functioned for fibroin synthesis and secretion. This suggested the fibroin synthesis and secretion procedure of the pos

关键词： silkworm Bombyx mori L., expressed sequence tag (EST ), silkgland, gene expression and regulation, fibroin.

Polymorphism identification of carotenoid binding protein gene transcription in the silkworm, Bombyx mori

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Advances in Bioscience and Biotechnology 2012年第2期3卷 107-114页

作者： Yanshan Niu Feifei Long Yanghu Sima Shiqing Xu National Engineering Laboratory for Modern Silk Department of Applied Biology Medical College Soochow University Suzhou China

Carotenoids play important and diverse roles in insects and their uptake and transport rely on carotenoid binding protein (CBP). The study excavated a cluster of CBP-like transcripts, including full CBP from all of the six yellow cocoon Bombyx strains investigated. Sequencing of 54 cDNA clones revealed 17 different types of transcripts which derived from alternative splicing of CBP gene locus. Five of the novel transcripts were similar with spatial and temporal distribution patterns to CBP, but their expression levels were relatively lower. The author disclosed two more novel alternative spliced transcripts with different transcription start sites from CBP in the 5’ UTRs as well as 11 SNP sites neighboring intron 1 after amplification and sequencing. qRT-PCR analysis gave evidence that relatively more mRNA was transcribed from A-type CBP gene than that from B-type in tissues like silk gland and midgut. Sequences of A- and B-type CBP genes were different in length of domains neighboring the 5’ UTR, thus their mRNA varied both in quantity and transcript types. The SNPs surrounding intron 1 can serve as stable markers to distinguish transcripts from the two isoforms, and they can be used for molecular marker assisted selection.

关键词： Carotenoid-Binding Protein Gene Alternative Splicing Transcripts Single Nucleotide Polymorphism Bombyx mori

INHIBITORY EFFECT OF POLY I:C AND 2′,5′-OLIGO (A) ON CYTOPLASMIC POLYHEDROSIS VIRUS OF SILKWORM, BOMBYX mori L.

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Chinese Science Bulletin 1983年第5期28卷 687-690页

作者：钟文彪黄自然卢蕴良刘新垣娄艳春 Department of Sericulture South China Agricultural College Guangzhou Shanghai Biochemistry Institute Academia Sinica Shanghai Biochemistry Institute Academia Sinicane of the major diseases in sericulture is the mid gut polyhedrosis in which the pathogen is the cytoplasmic polyhedrosis virus (CPV). After the silkworm larvae had been fed or injected with Poly Ⅰ: C or 2' 5'-oligo (A) (2' 5'-P_3A_3) silkworms increased their ability to inhibit CPV eventually the attack rate of the silkworm decreased by about 40-50%. Moreover it was found that Poly Ⅰ: C was able to induce some new proteins which seemed to be connected with the antiviral activity of the silkworm larva.

One of the major diseases in sericulture is the mid gut polyhedrosis, in which the pathogen is the cytoplasmic polyhedrosis virus （CPV）. After the silkworm larvae had been fed or injected with Poly Ⅰ: C or 2′, 5′-oligo （A）（2′, 5′-P3A3）, silkworms increased their ability to inhibit CPV, eventually, the attack rate of the silkworm decreased by about 40—50％. Moreover, it was found that Poly Ⅰ: C was able to induce some new proteins which seemed to be connected with the antiviral activity of the silkworm larva.

关键词： silkworm oligo larvae cytoplasmic antiviral BOMBYX mori L OLIGO inhibit eventually pathogen

mori SEIKI 1500S车铣复合机床后置处理算法的研究

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组合机床与自动化加工技术 2016年第12期 49-51页

作者：唐清春李科辉黎国强广西科技大学工程训练中心广西柳州545006

车铣复合机床与传统的五轴联动机床在结构上有区别,所控制的轴数超过5个。因此,在开发后置处理过程当中,与传统的五轴机床有较大区别,需要根据不同的功能来分析不同的运动链。文章主要以1500S车铣复合机床为例,详细介绍该类机床后置处... 详细信息

车铣复合机床与传统的五轴联动机床在结构上有区别,所控制的轴数超过5个。因此,在开发后置处理过程当中,与传统的五轴机床有较大区别,需要根据不同的功能来分析不同的运动链。文章主要以1500S车铣复合机床为例,详细介绍该类机床后置处理开发过程。首先建立mori SEIKI车铣数控机床的运动坐标系,根据运动链分析及坐标变换,推导出mori SEIKI机床的转角计算公式和坐标变换公式,分析了回转角在各个象限的取值;利用JAVA语言开发出mori SEIKI 1500S数控机床的专用后置处理软件,并通过某叶片仿真加工验证了该后置处理算法的正确性。该研究不仅为用户解决了软件使用问题,也为车铣复合类多轴机床提供了后置处理开发思路。

关键词： mori SEIKI 车铣复合机床运动求解坐标变换回转角取值