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Prevalence and methodologies for detection,characterization and subtyping of Listeria monocytogenes and *** in foods and environmental sources

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Food Science and Human Wellness 2017年第3期6卷 97-120页

作者： Jin-Qiang Chena Patrick Regan Pongpan Laksanalamai Stephanie Healey Zonglin Hua Winchester Engineering Analytical Center(WEAC)HFR-NE460Office of Regulatory AffairsUS Food and Drug Administration109 Holton StreetWinchesterMA 01890USA MD DHMH Laboratories Administration Division of MicrobiologyRoom 5281770 Ashland Ave.BaltimoreMD 21205USA

Listeria monocytogenes,one of the most important foodborne pathogens,can cause listeriosis,a lethal disease for ***,which is closely related to ***,is also widely distributed in nature and infects mainly warm-blooded ruminants,causing economic ***,there are high priority needs for methodologies for rapid,specific,cost-effective and accurate detection,characterization and subtyping of *** and *** in foods and environmental *** this review,we(A)described *** and ***,world-wide incidence of listeriosis,and prevalence of various *** strains in food and environmental sources;(B)comprehensively reviewed different types of traditional and newly developed methodologies,including culture-based,antigen/antibody-based,LOOP-mediated isothermal amplification,matrix-assisted laser desorption ionization-time of flight-mass spectrometry,DNA microarray,and genomic sequencing for detection and characterization of *** in foods and environmental sources;(C)comprehensively summarized different subtyping methodologies,including pulsed-field gel electrophoresis,multi-locus sequence typing,ribotyping,and phage-typing,and whole genomic sequencing *** subtyping of *** strains from food and environmental sources;and(D)described the applications of these methodologies in detection and subtyping of *** in foods and food processing facilities.

关键词： Foodborne pathogens Listeria monocytogenes Listeria ivanovii molecular characterization Subtyping

Mayaro fever:A brief review on the immune profile

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Asian Pacific Journal of Tropical Biomedicine 2020年第3期10卷 95-100页

作者： Jean Moisés Ferreira Danielly Santos Campos-Ferreira Elaine Virgínia Martins de Sousa Figueiredo Jose Luizde Lima Filho Laboratório de Imunopatologia Keizo Asami-LIKA Centro de BiociênciasUniversidade Federal de Pernambuco(UFPE)50670-901RecifePernambucoBrazil Laboratório de Biologia molecular e Expressao Genica-LABMEG Departamento de Ciências BiológicasUniversidade Federal de Alagoas(UFAL)Campus Arapiraca57300-970ArapiracaAlagoasBrazil

Mayaro virus is an emergent alphavirus that infects humans,leading to Mayaro *** fifty percent of infected patients develop arthritis symptoms in the recovery phase,a phase that can last up to a *** literature about Mayaro virus infection and its immune response is scarce,which may hamper the development of treatment *** summarize changes in cytokines and chemokines in the acute and recovery phase in Mayaro virus infected patients,and relate this molecular characterization with the immune *** and IL-12/p70 show pronounced changes in patients in the acute phase,suggesting the development of cellular immunity and Th1 ***-6,IL-7,CXCL8/IL-8,IL-13,IL-17,and IFN-γare elevated in patients with arthritis symptoms in the long-term recovery phase,which may be related to the continuous inflammatory process,a possible Th2 inhibiting and promoting Th17 *** few studies discuss the issue,with a small number of patients and different backgrounds,inflammatory and immune response and manifestations seem to be closely *** information may help to develop the appropriate treatment strategies in Mayaro virus ***,we analyzed and summarized data available in literature.

关键词： Emergent virus Mayaro virus Cytokines Chemokine molecular characterization

The journey of personalizing gastric cancer treatment

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Chinese Journal of Cancer 2016年第8期35卷 391-392页

作者： Li Yan US Chinese Anti-Cancer Association MartinezCA 94553USA Beijing Cancer Hospital and Institute Peking University School of OncologyBeijing 100142P. R.China

Gastric cancer ranks the fourth most prevalent malignancy yet it is the second leading cause of cancer-related *** year,gastric cancer adds nearly 1 million new cancer cases,and 723,000 or 10% of cancer deaths to the global cancer ***,405,000 or 43% of the new cases and 325,000 or 45% of the deaths are in China,making gastric cancer a particularly challenging *** thematic series discusses the molecular classifications of gastric cancer by the Cancer Genome Atlas(TCGA) and the Asian Cancer Research Group(ACRG) as well as the implications in personalized therapeutic choices;discusses the evolution of gastric surgery and presents perspectives on surgical techniques in treating gastric cancer;and reviews current and emerging targeted agents as well as immunotherapies in treating gastric *** these advancements in molecular characterization,surgical intervention,and targeted and immunotherapies,gastric cancer will enter a personalized medicine era in the next 5 years.

关键词： Gastric cancer Surgery molecular characterization Personalized medicine

Neuroendocrine differentiation of prostate cancer

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Asian Journal of Andrology 2013年第3期15卷 328-332页

作者： Zhen Li Clark J Chen Jason K Wang Elaine Hsia Wei Li Jill Squires Yin Sun Jiaoti Huang Departments of Pathology and Urology Jonsson Comprehensive Cancer Center and Broad Center for Regenerative Medicine and Stem Cell Research David Geffen School of Medicine at UCLA Los Angeles CA 90095-1732. USA

Benign prostate and prostatic adeno- carcinoma contain rare quiescentneuroendocrine cells while small cell neu- roendocrine carcinoma （SCNC）, a variant form of prostate cancer, contains highly proliferative neuroendocrine tumor cells. We provide evidence that IL-8-CXCR2- P53 pathway keeps the NE cells in a quiescent state normally. P53 mutation inactivates this pathway, resulting in hyper-proliferation and aggressive beha- vior of the NE cells in SCNC. Therefore, we have identified potential cells of origin and a molecular target for prostatic SCNC that are different from those of adenocarci- noma, which explains SCNC＇s distinct bio- logy and the clinical observation that it does not respond to hormonal therapy.

关键词： SMALL-CELL CARCINOMA ANDROGEN-INDEPENDENT GROWTH molecular characterization CXCR2 RECEPTOR MOUSE MODEL TRANSDIFFERENTIATION ADENOCARCINOMA INTERLEUKIN-8 PHENOTYPE LNCAP

A Review of Prevalence, Antimicrobial Susceptibility Patterns and molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) in the Caribbean

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Advances in Microbiology 2022年第8期12卷 459-480页

作者： Joshua B. Owolabi Simeon K. Olatunde Department of molecular and Cellular Sciences All Saints University School of Medicine Roseau Dominica

Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen causing infections resulting in severe morbidity and mortality worldwide. To date, the true nature and extent of MRSA infections in the Caribbean are not well understood. This is a review of the limited studies in the Caribbean describing the prevalence, epidemiology, and molecular characteristics of MRSA in hospitalized and non-hospitalized patients. Relevant articles were searched and extracted from PubMed and Mendeley and a narrative review of the findings was constructed. An aggregate of 24 articles, from 1999 to 2020, was found from 10 of 27 countries. Majority of the studies were from Trinidad and Tobago (29%) and Jamaica (21%) while 50% were from Barbados, Dominican Republic, Martinique, Haiti, Cuba, St. Kits & Nevis, Guadeloupe, and Guyana. Approximately 75% of investigations were conducted on hospitalized patients versus 20% on outpatients. The data revealed geographical differences in the prevalence of MRSA within the Caribbean;20% - 100% of Staphylococcus aureus clinical isolates from hospitalized patients and outpatients were resistant to methicillin, macrolides, and fluoroquinolones, but susceptible to several non-beta lactam antibiotics, due to the widespread occurrence of CA-MRSA clone ST8 SCCmec IV, PVL positive. There was moderate prevalence of ST72 SCCmec V (14% - 25%) in both hospital and community settings in a few of the countries while ST30 SCCmec IV, PVL positive, was moderately prevalent (27%) only in Dominican Republic. Also, there was moderate prevalence of HA-MRSA ST5 SCCmec II (18%) in community settings in the Dominican Republic and Martinique, but high prevalence of HA-MRSA ST239 SCCmec III (60%) in hospitalized patients in Cuba and Trinidad & Tobago. The epidemiologic profile of MRSA in both hospital and community settings is changing in the Caribbean. Epidemiological studies on outpatient settings and the implementation of stringent hospital infection control measu

关键词： MRSA Prevalence Epidemiology molecular characterization Caribbean

molecular Screening of Rice Cultivated in Benin for the Identification of Xanthomonas oryzae Pv. oryzae and Bacterial Leaf Blight Resistance Genes

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Advances in Bioscience and Biotechnology 2023年第12期14卷 514-533页

作者： Chimène Nadège Mahoussi Nanoukon Koffi David Montcho Hambada Antoine Abel Missihoun Kéllya Laurinzo Déguénon Bignon Meyrix Pamela Franzel Loumédjinon Bana Wêtè Déré Félicité Bio Emilienne Zinsou Réel Gael Fael Houngbélagnon Amed Sèmèvo Havivi Lamine Baba-Moussa Lambert Gustave Djédatin Laboratory of molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnolo-gies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of Plant Horticultural and Forestry Sciences National University of Agriculture Kétou Benin Laboratory of Molécular Genetics and Genome Analysis Department of Genetics and Biotechnology Faculty of Science and Technology (FAST) University of Abomey-Calavi Cotonou Benin Laboratory of Biology and molecular Typing in Microbiology Department of Biochemistry and Cell Biology University of Abomey-Calavi Abomey-Calavi Benin

One of the most devastating diseases of rice worldwide is bacterial blight (BLB) caused by Xanthomonas oryzae pv. Oryzae (Xoo). In Benin, Xoo was first described in 2013 on wild rice Oryzae longistaminata. So far, no study has been done on Beninese Xoo strains. We do not know whether the pathogen has already passed into the rice varieties grown, or if they are exposed to other bacteria. Whereas the use of resistant varieties, carrying resistance genes, is the only highly effective and environmentally friendly way to control this disease, no information is available on these Xoo resistance genes in rice varieties grown in Benin apart from the one we recently. This study aims to identify Beninese Xoo strains, causing BLB and screen rice varieties grown in Benin for the main resistance genes. Diseased rice leaves showing typical symptoms of fire blight collected from different rice fields in the three phytogeographic areas of Benin were analyzed by PCR for Xoo-specific sequence identification. Furthermore, seventy-five collected rice accessions were screened to identify xa5, Xa7, xa13, and Xa21 resistance genes to Xoo. The results reveal that Xanthomonas oryzae was identified in two fields in Banikouara and one in Malanville. On the other hand, Sphingomonas sp. has been identified in several other rice fields in Benin. Forty-seven of seventy-five rice accessions examined (62.66%) carried Xoo resistance genes with 3 (4%) and 40 (53.33%) of xa5 and Xa21 respectively. None of the accessions had either Xa7 or xa13 resistance genes. Three accessions possess both xa5 and Xa21 genes. Isogenic lines IRBB60 and IRBB21, supposed to be a positive control, presented a Xoo sensitivity allele. These results indicate that Xoo has moved from the wild rice variety to the cultivated variety in northern Benin and varietal improvement programs must be implemented with varieties having several resistance genes for the efficient response against a possible BLB pandemic in Benin.

关键词： Bacterial Blight Xanthomonas oryzae Pv. oryzae molecular characterization Resistance Genes

Isolation and molecular characterizetion of a porcine teschovirus 1 isolate from China

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Isolation and molecular characterizetion of a porcine tescho...

Comparison of RF3 <i>B. juncea</i>to RF3 <i>B. napus</i>

中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第七次研讨会

作者： L. FENG1, H.Y. SHI1, S.W. LIU1, B.P. WU1, J.F. CHEN1, D.B. SUN1, Y.E. TONG1, M.S. FU2, Y.F. WANG1, G.Z. TONG1 (1.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Ma Duan St., Harbin 150001, P.R. China 2. Tong Liao Animal Science and Veterinary Research Institute, Inner Mongolia Province 028000, P.R. China)

Porcine teschovirus 1 (PTV-1) (Swine/CH/IMH/03) was isolated from piglets in a farm in Inner Mongolia Province, P.R. China. It was confirmed by electron microscopy, RT-PCR, and sequencing. Comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein showed that PTV Swine/CH/IMH/03 strain is PTV-1. The isolated virus has closest relationship with Talfan strain, they shared 98.9 % and 99.5 % homology of amino acids and nucleotides, respectively, in the ORF of polyprotein. To our knowledge, this is the first report about isolation and identification of a PTV in China.

关键词： isolation molecular characterization Porcine teschovirus 1

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Food and Nutrition Sciences 2021年第11期12卷 1041-1061页

作者： Sandra J. W. Mackie BASF Corporation Research Triangle Park NC USA

RF3 Brassica napus L. (InVigorTM Canola) has been in commercial cultivation since 1997. RF3 B. napus was produced by Agrobacterium-mediated transformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of Brassica juncea L. (Czern & Coss), a close relative of B. napus, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 B. juncea via conventional breeding with RF3 B. napus to provide similar herbicide tolerance in the B. juncea species. The insertion event RF3 contains the bar gene (origin Streptomyces hygroscopicus) coding for phosphinothricin acetyl transferase (PAT/bar) protein which confers tolerance to glufosinate-ammonium herbicides. RF3 also contains the barstar gene (origin Bacillus amyloliquefaciens), coding for the Barstar protein, which is an inhibitor of the Barnase protein. In the absence of Barnase, there is no impact of Barstar. Many safety studies have been conducted during the development of the canola quality lines of RF3 B. napus and RF3 B. juncea. This report seeks to compare the two species based on the scientific data generated during the safety assessments of RF3 B. napus and RF3 B. juncea and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.

关键词： Canola brassica juncea napus RF3 Composition Protein Expression molecular characterization

Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers

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American Journal of molecular Biology 2016年第1期6卷 12-24页

作者： Hafiz A. Salami Kamirou Chabi Sika Wilfrid Padonou Djima Aly Chabi Yallou Adolphe Adjanohoun Simeon Kotchoni Lamine Baba-Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Programme de Technologie Agricole (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Bénin Centre de Recherches Agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Cotonou Bénin Centre de Recherches Agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) Ina Bénin Departement of Biology Rutgers University Camden USA

Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize inbred lines. The aim of this study was to evaluate the genetic diversity of Benin’s maize accessions by SSR marker. Thus, one hundred eighty seven maize accessions from three areas (South, Center and North) were analyzed using three SSR markers. A total of 227 polymorphic bands were produced and showed high genetic diversity (Shannon index = 0.51). The polymorphic information content (PIC) values for the SSR loci ranged from 0.58 to 0.81, with an average of 0.71. Genetic distance-based UPGMA dendrogram showed a genetic differentiation between accessions and they were grouped into four clusters in each area. This work provides necessary information that can be used not only to improve the maize production and conservation but also to better manage genetic species resources in Benin.

关键词： SSR PCR molecular characterization LZea mays L. Benin