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Effects of AZT and RNA-protein complex (FA-2-b-β) extracted from Liang Jin mushroom on apoptosis of gastric cancer cells

引用

World Journal of Gastroenterology 2007年第31期13卷 4185-4191页

作者： Yan-Qing Sun Zi-Ren Wang Tian-Kang Guo Ya-Ming Xi Che Chen, Jin-Wang SchoolofLifeScience.LanzhouUniversity Lanzhou730000GansuProvinceChina DepartmentofSurgery ThePeople′s HospitalofGansuProvinceLanzhou730000GansuProvinceChina DepartmentofHematology FirstAffiliatedHospitalofLanzhouUniversityLanzhou730000GansuProvinceChina ScientificResearch Center GansuCollegeofTraditionalChineseMedicineLanzhou730000GansuProvinceChina DepartmentofHematology LanzhouUniversityLanzhou730000GansuProvinceChina

To investigate the synergistic effects of 3′-azido-3′- deoxythymidine （AZT） and FA-2-b-β extracted from Ling Jin mushroom on apoptosis of gastric cancer cells mkn45 in vitro. METHODS： Ml-I- analysis was made to examine the inhibition rate of mkn45 cells treated with AZT （2.5, 5, 10 and 20 mg/L） and FA-2-b-13 （5, 10, 20 and 40 mg/L） singly and combinatively for 24, 48 and 72 h. Apoptotic effects were evaluated by morphological methods, DNA agarose gel electrophoresis and flow cytometry, respectively. Telomerase activity was estimated by TRAP- ELISA. The mRNA expression of caspase-3 and Bcl-2 were detected by RT-PCR. RESULTS： AZT and FA-2-b-13 could significantly inhibit mkn45 cell proliferation and induce its apoptosis. mkn45 cells were inhibited in dose- and time- dependent manner. The inhibition effect of AZT combined with FA-2- b-β was obviously better than that used singly （0.469 ＋ 0.022 vs 1.075 4- 0.055, P 〈 0.05, 0.325 4- 0.029 vs 0.469 ＋ 0.022 P 〈 0.01）. AZT used singly and combination of FA-2-b-β could decrease the activity of tumor cell telomerase, and AZT has synergistic function with FA- 2-b-β. A certain concentration of AZT could up-regulate the expression of caspase-3 mRNA （r = 0.9969, P 〈 0.01）, which was positively related to apoptosis rate, and could down-regulate the expression of Bcl-2 mRNA, which was negatively related to apoptosis rate （r = 0.926, P 〈 0.01）. Furthermore, the effect of AZT combined with FA-2-b-13 was significantly higher than that used singly. CONCLUSION： Combination of AZT and FA-2-b-β has an obviously synergetic effect in the gastric cancer cells mkn45, which has provided a new approach to the treatment of gastric cancer clinically.

关键词： AZT FA-2-b-β Apoptosis Telomerase Bcl-2 Caspase-3 mkn45 cell

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