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Role of microsatellite instability in occurrence of colorectal carcinoma and its prognostic significance

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Journal of Medical Colleges of PLA(China) 1999年第2期14卷 134-137页

作者：阎晓初陈意生柳凤轩罗元辉鲁荣

Objective: To explore the role of microsatellite instability (MSI ) in the occurrence of colorectal carcinoma and the relationship between MSI and prognosis of the carcinoma. Methods: MSI at 6 loci of D18S34. D17S799. D55409. TCF--2, P53 and Rb in 56 cases of colorectal carcinoma were detected with PCRSSLP. Results: The total positive rate of MSI was 44. 64 %. The 3-or 5-year survival rate was higher in the cases with MSI than in those without. Conclusion: MSI may be another molecular mechanism of occurrence of colorectal carcinoma. The cases of colorectal carcinoma with MSI have better biological behavior and outcome. Therefore. the detection of MSI might be useful for prediction of the prognosis of colorectal carcinoma.

关键词： colorectal carcinoma: microsatellite instability prognosis

Genetic Quality Analysis of Xiphophorus helleri RR-B Strain Using 18 microsatellite Loci

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Agricultural Science & Technology 2008年第6期9卷 55-58,101页

作者：刘宇飞白俊杰全迎春叶星黄志斌中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室广东广州510380 上海海洋大学水产与生命学院上海200090

[Objective] The study aimed to further understand the genetic quality of Xiphophorus helleri RR-B strain and estimate the feasibility of microsatellite markers in genetic monitoring of aquatic laboratory animals.[Method] Eighteen microsatellite loci which had polymorphism in wild X. helleri were used for genetic quality analysis of RR-B strain (red eyes and red body) by PCR. [Result] All these 18 microsatellite loci displayed single allelic gene band in 30 individuals of X. helleri RR-B strain and the average homozygote rate reached 100%. [Conclusion] These loci could be used as markers in genetic monitoring of X. helleri RR-B strain, which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations. This study provided basic data for establishing the molecular genetic monitoring standard of aquatic laboratory animals.

关键词： Xiphophorus helleri Inbred strain microsatellite Genetic monitoring

Genetic Analysis of Three Populations of Barbados Blackbelly Sheep at microsatellite Loci

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Journal of Agricultural Science and Technology(A) 2011年第8期1卷 1187-1191页

作者： L. McClean L. Waterman C. Roberts The University of the West Indies Cave Hill Barbados Caribbean Agricultural Research and Development Institute Barbados Unit Cave Hill Barbados

The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers on some Caribbean islands. However, the determination of what is a purebred BBB sheep has long been a contentious issue because of the subjectivity. It is therefore necessary to have a more reliable means of identifying what is a purebred BBB sheep for purposes of conservation and breed improvement. Three geographically isolated populations of BBB sheep were genetically analysed at 19 microsatellite loci. Eighteen of the microsatellites were found to be useful for genetic analysis of the BBB sheep, based on polymorphic information content （PIC） values （0.5 〈 PIC 〉 0.25）. One microsatellite was not useful for genetic analysis based on PIC （0.111）, however it is considered to be a locus of interest because it is fully inbred （f = 1） and homozygous in all of the populations analysed. The analysis also showed that the purebred population of BBB sheep can be distinguished from other populations by formation of distinct clusters when subjected to analysis of molecular variance （AMOVA） and the construction of a rooted consensus tree using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships.

关键词： Barbados blackbelly sheep microsatellite polymorphism information content.

An Example of Yak Paternity Assignment by microsatellite Genotyping

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中国草食动物 2005年第Z2期25卷 225-226,208页

作者：王敏强 Department of Bio-technique and Bio-Science Yantai University P. R. China International Livestock Research Institute (ILRI) Institute for Animal Science Mariensee(FAL) Germany

12 cattle microsatellite DNA markers were used for establishing the paternity of 4 Datong Yak *** results indicated that paternity can be assigned by use of exclusion method directely when the mother is clearly known and data of all likely sires were obtained. The more the alleles of a locus possessed, the more effectively the paternity can be assigned. Not only the microsatellite DNA marker is a promising tool in establishment of yak detail pedigrees, but also can provide us information of reproductive efficiency of the bull(s) based on the numbers of calves it produced in the herd, which will be useful in speeding up the processes of yak genetics improvement.

关键词： microsatellite Markers Yak Breeding Paternity assignment.

Genetic Diversity Analysis of Guizhou Black Goat Using microsatellite DNA Markers

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Agricultural Biotechnology 2016年第1期5卷 30-32页

作者： Chaozhi SHU Xue HAN Lina LI Animal Husbandry and Veterinary Research Institute of Guizhou Province Guiyang 550005China

In this study, the genetic diversity of Guizhou black goat was analyzed by using eight microsatellite DNA markers, including MAF33, OarFCB48, OarF- CB3IM, BM1258, MAF70, OarFCB11, RM088 and AEIO1. According to the results, a total of 43 alleles were found at eight microsatellite loci in Guizhou black goat population. The average heterozygosity was 0. 679 2 in Guizhou black goat population. microsatellite locus MAF70 had the highest hetemzygosity （0.780 7 ）, and locus AE101 exhibited the lowest heterozygosity （0.545 5 ）. The average polymorphism information content （PIC） was 0. 626 7 in Guizhou black goat popula- tion, which was higher than O. 5, indicating high levels of polymorphism.

关键词： Guizhou black goat microsatellite Genetic diversity

The Identification and Assessment on Genetic Characteristics in Grading Breeding Sheep Populations with microsatellite Markers

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Agricultural Science & Technology 2008年第6期9卷 21-24页

作者：金鑫苏蕊张文广李金泉内蒙古农业大学动物科学与医学学院内蒙古呼和浩特010018

[Objective] The aim of the research was to identify and assess the genetic characteristics of grading breeding sheep populations in Ba Yan Nur City. [Method] Genetic polymorphism and aggregation of seven sheep populations, including three breeding sheep populations (breeding F1, F2 and Bamei mutton sheep), three introduced mutton sheep breeds (Texel, Dorset and German Merino sheep) and one local female parent population (Mongolia sheep), were assessed using 10 microsatellite markers. [Result] By cluster analysis, the seven sheep populations can be divided into two groups. The F1 and German Merino sheep were closely related, which were clustered with F2, Bamei mutton sheep and Mongolia sheep to form one group while Texel and Dorset to form another group. The genetic aggregation of the seven breeds was assessed by Bayesian discrimination. And the results show that the genetic aggregation of F1 and F2 were lower while that of Bamei mutton sheep, Texel, Dorset and German Merino sheep were higher. [Conclusion] Better genetic stability has been formed in Bamei mutton sheep.

关键词： Grading breeding microsatellite markers Genetic polymorphism Bayesian discrimination

Polymorphism of microsatellite sequence within signal transduction coding genes in phytopathogenic fungus, Magnaporthe grisea

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Journal of Life Sciences 2007年第1期1卷 18-21页

作者： LIU Lin LI Cheng-yun YANG Jing LI Jin-bin SU Yuan WANG Yun-yue ZHU You-yong Key Laboratory of Agricultural Biodiversity and Pest Management of China Education Ministry Yunnan Agricultural University Kunming 650201 China Agriculture Environment and Resource Research Institute Yunnan Academy of Agricultural Sciences Kunming 650205 China

Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations （including japonica grown zone, indica grown zone） and rice varieties. Preliminary results indicated that each locus resolved multiple alleles ranging from three to fourteen. The results showed that these SSR-containing genes are also polymorphic in the nature population.

关键词： Magnaporthe grisea signal transduction microsatellite

Development of Fifteen Novel microsatellite Markers from Rock Bream (<i>Oplegnathus fasciatus</i>)

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Open Journal of Genetics 2014年第4期4卷 287-291页

作者： Jong-Oh Kim Wi-Sik Kim Myung-Joo Oh Department of Aqualife Medicine Chonnam National University Yeosu Korea

Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.

关键词： Oplegnathus fasciatus Rock Bream microsatellite Markers Population Genetics

Brazilian Buffalo Genetic Variability by Cross-Specific microsatellite Set

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Journal of Agricultural Science and Technology(B) 2011年第7期1卷 1008-1012页

作者： A. Rogberg Mufioz L. Viana Texeira E. E. Villegas-Castagnasso C. Salviano Teixeira P. Peral-Garcia D. A.Andrade De Oliveira G. Giovambattista Veterinary Genetics Institute （IGEVET） CCT La Plata-Veterinary Science Faculty National University of La Plata-CONICET LaPlata Buenos Aires Province B19OOA VW CC 296 Argentina Laboratory of Genetics Veterinary Science Faculty Federal University of Minas Gerais Belo Horizonte Minas Gerais State31270-010 Brasil

Buffaloes （Bubalus bubalis） are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific （cattle） markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic （58%）. The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres （China and India）, but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.

关键词： Buffalo microsatellite variability DNA markers traceability.