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Simulation of a soft-gamma-ray polarimeter on board a microsatellite

Nuclear Science and Techniques 2023年第11期34卷 64-76页

作者： Xiang‑Man Liu Shu‑Wen Tang Wei Wang Yu‑Hong Yu Zhi‑Yu Sun Yong‑Jie Zhang Fang Fang Duo Yan Shi‑Tao Wang Xue‑Heng Zhang Shu‑Ya Jin Bi‑Tao Hu Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China Department of Astronomy School of Physics and TechnologyWuhan UniversityWuhan 430072China Wuhan University‑National Astronomical Observatories Joint Center for Astronomy Wuhan UniversityWuhan 430072China

Gamma-ray polarimetry is a new and prospective tool for studying extremely high-energy celestial objects and is of great significance for the field of *** the rapid development of microsatellite technology,the advantages of space exploration have become increasingly ***,we simulated a soft-gamma-ray polarimeter for a microsatellite based on the Compton scattering *** performed detailed Monte Carlo simulations using monoenergetic gamma-ray linear-polarization sources and Crab-like sources in the energy range of 0.1-10 MeV considering the orbital *** polarimeter exhibited excellent polarization detection *** modulation factor was 0.80±0.01,and the polarization angles were accurate within an error of 0.2°at 200 keV for on-axis *** the Crab-like sources for on-axis incidence,the polarization degrees were consistent with the set values within the error tolerance,the modulation factor was 0.76±0.01,and the minimum detectable polarization reached 2.4%at 3σfor an observation time of10^(6) ***,the polarimeter exhibited recoil electron tracking,imaging,and powerful background suppression in a large field of view(FoV;∼2πsr).The proposed polarimeter meets the requirements of a space soft-gamma-ray polarization detector and has promising research prospects.

关键词： Soft gamma ray Polarization Compton scattering microsatellite Monte Carlo simulation

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Genome-wide development of interspecific microsatellite markers for Saccharum officinarum and Saccharum spontaneum

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Journal of Integrative Agriculture 2022年第11期21卷 3230-3244页

作者： LIU Lei WANG Heng-bo LI Yi-han CHEN Shu-qi WU Ming-xing DOU Mei-jie QI Yi-yin FANG Jing-ping ZHANG Ji-sen Center for Genomics and Biotechnology Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyKey Laboratory of GeneticsFujian Agriculture and Forestry UniversityFuzhouFujian 350002P.R.China State Key Lab for Conservation and Utilization of Subtropical Agro-Biological Resources&Guangxi Key Lab for Sugarcane Biology Guangxi UniversityNanning 530004P.R.China College of Life Sciences Fujian Normal UniversityFuzhou 350007P.R.China

Sugarcane has a large,complex,polyploid genome that has hindered the progress of genomic research and molecular marker-assisted *** user-friendly SSR markers have attracted considerable attention owing to their ideal genetic ***,these markers were not characterized and developed at the genome-wide scale due to the previously lacking high-quality chromosome-level assembled sugarcane *** this present study,744305and 361638 candidate SSRs were identified from the genomes of *** and ***,*** verified the reliability of the predicted SSRs by using 1200 interspecific SSR primer pairs to detect polymorphisms among 11 representative accessions of Saccharum,including ***,***,***,and modern sugarcane *** results showed that 660 SSR markers displayed interspecific polymorphisms among these ***,100 SSRs were randomly selected to detect the genetic diversity for 39 representative Saccharum accessions.A total of 320 alleles were generated using 100 polymorphic primers,with each marker ranging from two to seven *** genetic diversity analysis revealed that these accessions were distributed in four main groups,including group I(14 *** accessions),group II(two *** accessions),group III(18 modern sugarcane hybrid accessions),and group IV(five *** accessions).Experimental verification supported the reliability of the SSR markers based on genome-wide *** development of a large number of SSR markers based on wet experiments is valuable for genetic studies,including genetic linkage maps,comparative genome analysis,genome-wide association studies,and marker-assisted selection in Saccharum.

关键词： Saccharum genome-wide simple repeat sequences(SSR) microsatellite molecular markers

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microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

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Chinese Science Bulletin 2000年第20期45卷 1883-1885页

作者： DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan SUN Qingyuan LI Guangpeng WANG Minkang LIU Yilong KANG Li HAN Zhiming SONG Xiangfen LI Jingsong CHEN Yuchun Laboratory of Cellular and Molecular Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China Fuzhou Panda Institute Center Fuzhou 350001China 不详

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.

关键词： giant panda (Ailuropoda melanoleuca) interspecies cloning microsatellite DNA nucleus.

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The analysis of genetic diversity and differentiation of six Chinese cattle populations using microsatellite markers

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Journal of Genetics and Genomics 2008年第1期35卷 25-32页

作者： Yongjiang Mao Hong Chang Zhangping Yang Liu Zhang Ming Xu Guobin Chang Wei Sun Guangming Song Dejun Ji Animal Science and Technology College Yangzhou University Yangzhou 225009 China

A total of 321 individuals from six cattle populations of four species in a bovine subfamily in China were studied using 12 pairs of microsatellite markers. The genetic diversities within and between populations were calculated. The phylogenetic trees were constructed by （δμ）^2 and DA distances, and the divergence times between populations were estimated by （δμ）^2. Altogether, 144 microsatellite alleles were detected including 24 private alleles and nine shared alleles. Chinese Holstein had the largest number of private alleles （10）, whereas Bohai black and Buffalo had the smallest number of private alleles （2）. Chinese Holstein showed the highest genetic variability. Its observed number of alleles （Na）, mean effective number of alleles （MNA）, and mean heterozygosity （He） were 7.7500, 4.9722, and 0.7719, respectively, whereas, the Buffalo and Yak showed low genetic variability. In the phylogenetic trees, Luxi and Holstein grouped first, followed by Bohai and Minnan. Yak branched next and buffalo emerged as the most divergent population from other cattle populations. Luxi and Bohai were estimated to have diverged 0.039-0.105 million years ago （MYA）, however, buffalo and Holstein diverged 0.501-1.337 MYA. The divergence time of Yak versus Minnan, Holstein and buffalo was 0.136-0.363, 0.273-0.729, and 0.326-0.600 MYA, respectively.

关键词： genetic diversity genetic differentiation microsatellite bovine subfamily

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Analysis of genetic diversity and population structure of Chinese yak breeds (Bos grunniens) using microsatellite markers

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Journal of Genetics and Genomics 2008年第4期35卷 233-238页

作者： Guixiang Zhang Weisheng Chen Ming Xue Zhigang Wang Hong Chang Xu Han Xinjun Liao Donglei Wang National Center of Preservation and Utilization of Genetic Resources of Domestic Animals and Forage National Animal Husbandry and Veterinary Service Beijing 100094 China NationalAnimal Husbandry Service Beijing 100026 China College of Animal Science and Technology Yangzhou University Yangzhou 225009 China

Nine Chinese yak breeds （Maiwa, Tianzhu White, Qinghai Plateau, Sibu, Zhongdian, Pali, Tibetan High Mountain, Jiulong, and Xinjiang） and Gayal were analyzed by means of 16 microsatellite markers to determine the level of genetic variation within populations, genetic relationship between populations, and population structure for each breed. A total of 206 microsatellite alleles were observed. Mean F-statistics （0.056） for 9 yak breeds indicated that 94.4% of the genetic variation was observed within yak breeds and 5.6% of the genetic variation existed amongst breeds. The Neighbor-Joining phylogenetic tree was constructed based on Nei＇s standard genetic distances and two clusters were obtained. The Gayal separated from the yaks far away and formed one cluster and 9 yak breeds were grouped together. The analysis of population structure for 9 yak breeds and the Gayal showed that they resulted in four clusters; one cluster includes yaks from Tibet Autonomous Region and Qinghai Province, one cluster combines Zhongdian, Maiwa, and Tianzhu White, and Jiulong and Xinjiang come into the third cluster. Pali was mainly in the first cluster （90%）, Jiulong was mainly in the second cluster （87.1%）, Zhongdian was primarily in the third cluster （83%）, and the other yak breeds were distributed in two to three clusters. The Gayal was positively left in the fourth cluster （99.3%）.

关键词： Chinese yak breeds microsatellite genetic diversity population structure

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microsatellite loci from Taxus chinensis var. mairei (Taxaceae), an endangered and economically important tree species in China

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Frontiers in Biology 2009年第2期4卷 214-216页

作者： Yuan ZHOU Guopei CHEN Yingjuan SU Zhiwei WANG Wuhan Botanical Garden/Wuhan Institute of Botany The Chinese Academy of SciencesWuhan 430074China State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Taxus chinensis *** is an endangered and economically important tree species in *** polymorphic microsatellite markers were developed and characterized using the fast isolation by amplified fragment length polymorphism(AFLP)of sequences containing repeats(FIASCO)*** of each locus was quantified for five natural populations from Hubei Province,*** number of alleles per locus varied from 6 to *** ranges of observed and expected heterozygosity were 0.493–1.000 and 0.440–0.845,*** are the first microsatellites reported for the family of Taxaceae and will be helpful for the ongoing population and conservation genetics research of Taxus chinensis ***.

关键词： Taxus chinensis var.mairei genetic variation microsatellite

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Using MiddRAD-seq data to develop polymorphic microsatellite markers for an endangered yew species

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Plant Diversity 2017年第5期39卷 294-299页

作者： Hantao Qin Guoqian Yang Jim Provan Jie Liu Lianming Gao Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201 China University of Chinese Academy of Sciences Beijing100049China Germplasm Bank of Wild Species in Southwest China Kunming Institute of BotanyChinese Academy of SciencesKunming650201China Institute of Biological Environmental and Rural Sciences (IBERS)Aberystwyth UniversityPengiaisAberystwythSY23 3DAUK

microsatellites are highly polymorphic markers which have been used in a wide range of genetic *** recent years, various sources of next-generation sequencing data have been used to develop new microsatellite loci, but compared with the more common shotgun genomic sequencing or transcriptome data, the potential utility of RAD-seq data for microsatellite ascertainment is comparatively *** this study, we employed MiddRAD-seq data to develop polymorphic microsatellite loci for the endangered yew species Taxus florinii. Of 8,823,053 clean reads generated for ten individuals of a population, 94,851(~1%) contained microsatellite motifs. These corresponded to 2993 unique loci, of which 526(~18%) exhibited polymorphism. Of which, 237 were suitable for designing microsatellite primer pairs, and 128 loci were randomly selected for PCR validation and microsatellite screening. Out of the 128 primer pairs, 16 loci gave clear, reproducible patterns, and were then screened and characterized in 24 individuals from two populations. The total number of alleles per locus ranged from two to ten(mean=4.875), and within-population expected heterozygosity from zero to 0.789(mean = 0.530),indicating that these microsatellite loci will be useful for population genetics and speciation studies of T. florinii. This study represents one of few examples to mine polymorphic microsatellite loci from ddRAD data.

关键词： MiddRAD-seq Endangered species microsatellite Next-generation sequencing Taxus florinii

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Location of Vibrio anguillarum resistance-associated trait loci in half-smooth tongue sole Cynoglossus semilaevis at its microsatellite linkage map

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Chinese Journal of Oceanology and Limnology 2016年第6期34卷 1309-1319页

作者：汤志宏郭栗刘洋邵长伟陈松林杨官品 College of Marine Life Sciences Ocean University of China Yellow Sea Fisheries Institute Chinese Academy of Fishery Sciences

A cultured female half-smooth tongue sole(C ynoglossus semilaevis) was crossed with a wild male, yielding the fi rst fi lial generation of pseudo-testcrossing from which 200 fi sh were randomly selected to locate the V ibrio anguillarum resistance trait in half-smooth tongue sole at its microsatellite linkage map. In total, 129 microsatellites were arrayed into 18 linkage groups, ≥4 each. The map reconstructed was 852.85 c M in length with an average spacing of 7.68 c M, covering 72.07% of that expected(1 183.35 cM). The V. anguillarum resistance trait was a composite rather than a unit trait, which was tentatively partitioned into Survival time in Hours After V. anguillarum Infection(SHAVI) and Immunity of V. A nguillarum Infection(IVAI). Above a logarithm of the odds(LOD) threshold of 2.5, 18 loci relative to SHAVI and 3 relative to IVAI were identifi ed. The 3 loci relative to IVAI explained 18.78%, 5.87% and 6.50% of the total phenotypic variation in immunity. The microsatellites bounding the 3 quantitative trait loci(QTLs) of IVAI may in future aid to the selection of V. anguillarum-immune half-smooth tongue sole varieties, and facilitate cloning the gene(s) controlling such immunity.

关键词： Cynoglossus semilaevis Vibrio anguillarum microsatellite 量的特点地点 QTL

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Assessment of Genetic Diversity of Drought Tolerant and Susceptible Rice Genotypes Using microsatellite Markers

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Rice science 2019年第4期26卷 239-247,I0005,I0010-I0014页

作者： Ravindra DONDE Jitendra KUMAR Gayatri GOUDA Manoj Kumar GUPTA Mitadru MUKHERJEE Sk Yasin BAKSH Pradosh MAHADANI Khirod Kumar SAHOO Lambodar BEHERA Sushanta Kumar DASH Crop Improvement Division Indian Council of Agricultural Research-National Rice Research Institute/Formerly-Central Rice Research Institute Cuttack Pin-753006.OdishaIndia Ravenshaw University Department of Biotechnology Cuttack Pin-753003Odisha India Department of Biotechnology & Bioinformatics Yogi Vemana University KadapaPin-516003 OdishaIndia

The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.

关键词： genetic diversity marker-assisted selection microsatellite marker rice drought stress submergence chromosome segment substitution line

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Molecular Grouping of Grateloupia Tissues Collected Along Chinese Coast and microsatellite Diversity Analysis of G. asiatica

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Journal of Ocean University of China 2018年第4期17卷 925-931页

作者： PENG Chong DING Haiyan TANG Zhihong GUO Li YANG Guanpin College of Marine Life Sciences Ocean University of China Institute of Evolution & Marine Biodiversity Ocean University of China Key Laboratory of Marine Genetics and Breeding of Chinese Ministry of Education Ocean University of China

Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological characteristics, making their morphological identification very difficult. In addition, few of the species diversity in this genus has been described before. In this study, phylogenetic analysis based on rbc L gene sequence was employed to group Grateloupia collected from three locations along Chinese coast. The microsatellites were also used to evaluate their genetic diversity. In total, the tissue parts of 6 putative species were collected from G. asiatica, G. livida, G. lanceolate, G. catenata, G. turuturu and G. filicina. In order to evaluate their genetic diversity and then conserve them better, 40 microsatellites available for Grateloupia were used to evaluate their genetic diversity, and 11 microsatellites were found to be applicable to determine the genetic diversity of G. asiatica. It was found that the genetic diversity of G. asiatica around Qingdao was very rich. We suggested that the species of genus Grateloupia should be identified based on rbc L phylogenetic analysis before the diversity evaluation with microsatellites. The microsatellites should be developed for each species of Grateloupia so that their genetic diversity can be evaluated appropriately.

关键词： Grateloupia rbc L molecular grouping microsatellite genetic diversity

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