检索结果-南通市图书馆

Insights into the changes of amino acids,microbial community,and enzymatic activities related with the nutrient quality of product during the composting of food waste

在线全文

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2023年第3期17卷 93-105页

作者： Ying Xue Keke Xiao Xiang Wu Mei Sun Yifei Liu Bei Ou Jiakuan Yang School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China

This study systematically investigated the changes of amino acids as the composting process of food waste *** is found that the addition of 0.3 g/g total solids of food waste achieved the highest seed germination index of the product(268%).The microbial community results indicated that the abundance of amino acid metabolism sequences remained at high levels during the whole composting *** was identified as the key amino acid related with the nutrient quality of product during the composting of food *** plant germination and hydroponic experiments found,that compared with those without the addition of proline,the addition of 50 mg/L proline increased seed germination rate by 20%,increased shoot length by 3%,increased root biomass of seedlings by 82%,and increased leaf biomass of seedlings by 76%,***,γ-Pseudomonadota,Chloroflexi and Planctomycetes were the key identified bacteria related with the increase of proline during the composting of food ***,the enzymatic tests of the activities of superoxide dismutase,peroxidase and malondialdehyde indicated that proline did not cause oxidative damage on the growth of *** study provided novel insights into the changes of amino acids,microbial community,and enzymatic activities related with the nutrient quality of product during the composting of food waste.

关键词： Composting Food waste Amino acids microbial community Enzymatic activity Nutrient quality

Anaerobic co-digestion of thermo-alkaline pretreated microalgae and sewage sludge: Methane potential and microbial community

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2023年第5期127卷 133-142页

作者： Jiaqi Fu Bing Yan Shuanglin Gui Yinxuan Fu Song Xia Institute of Energy Research Jiangxi Academy of SciencesNanchang 330096China

To improve methane production from sewage sludge(SS),co-digestion of SS and microalgae(MA)was studied and the application of thermo-alkaline pretreatment to MA was *** results showed that thermo-alkaline pretreatment at 90℃ for 120 min on MA was the optimum pretreatment ***,when the volatile solids(VS)ratio of SS and MA was 1:2,the methane yield reached maximum(368.94 mL/g VS).Fourier transform infrared(FT-IR)and thermogravimetric analysis confirmed the synergetic effects of thermoalkaline pretreated MA on its co-digestion with *** analyses of microbial community indicated that Methanobacterium and Methanosarcina were the dominant methanogens during the co-digestion ***,the relative abundance of Methanosarcina in thermoalkaline pretreated groups was higher compared to unpretreated *** microbial community structure might be affected by thermo-alkaline pretreatment rather than by the MA dosage in the co-digestion.

关键词： Anaerobic co-digestion Thermo-alkaline pretreatment Microalgae Sewage sludge microbial community

Investigation of physico-chemical properties and microbial community during poultry manure co-composting process

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2015年第2期27卷 81-94页

作者： Omar Farah Nadia Loo Yu Xiang Lee Yei Lie Dzulkornain Chairil Anuar Mohammed P.Mohd Afandi Samsu Azhari Baharuddin Department of Process and Food Engineering Faculty of Engineering Universiti Putra Malaysia Institute of Tropical Forestry and Forestry Products (INTROP) Putra Infoport Universiti Putra Malaysia

Co-composting of poultry manure and rubber wood sawdust was performed with the ratio of 2：1（V/V） for a period of 60 days. An investigation was carried out to study the extracellular enzymatic activities and structural degradation utilizing Fourier transform infrared spectroscopy（FT-IR）, thermogravimetry and differential thermal analysis（TG/DTA）and scanning electron microscopy（SEM）. The microbial succession was also determined by using denaturing gel gradient electrophoresis（DGGE）. The compost was able to reach its highest temperature of 71°C at day 3 and stabilized between 30 and 40°C for 8 ***, FPase and β-glucosidase acted synergistically in order to degrade the cellulosic substrate. The xylanase activities increased gradually during the composting and reached the peak value of 11.637 U/g on day 35, followed by a sharp decline. Both Li P and Mn P activities reached their peak values on day 35 with 0.431 and 0.132 U/g respectively. The FT-IR spectra revealed an increase in aromaticity and a decrease in aliphatic compounds such as carbohydrates as decomposition proceeded. TGA/DTG data exhibited significant changes in weight loss in compost samples, indicating degradation of organic matter. SEM micrographs showed higher amounts of parenchyma exposed on the surface of rubber wood sawdust at day 60, showing significant degradation. DGGE and 16 S r DNA analyses showed that Burkholderia sp., Pandoraea sp., and Pseudomonas sp. were present throughout the composting process. Ornithinibacillus sp. and Castellaniella ginsengisoli were only found in the initial stage of the composting, while different strains of Burkholderia sp. also occurred in the later stage of composting.

关键词： Composting Poultry manure Cellulose Denaturing gel gradient electrophoresis microbial community

Effect of CO_2 and N_2 on microbial community changes during column bioleaching of low-grade high pyrite-bearing chalcocite ore

在线全文

学校读者我要写书评

暂无评论

Journal of Central South University 2015年第12期22卷 4528-4535页

作者：陈勃伟武彪刘兴宇温建康 National Engineering Laboratory of Biohydrometallurgy General Research Institute for Nonferrous Metals

Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO_2 and N_*** samples were collected at day 43,64,85,106 and subjected to microbial community analysis by 16S rRNA gene clone *** analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus,Leptospirillum and *** of CO_2 and N_2 significantly impacted the microbial community *** CO_2 was aerated,the proportion of genus Acidithiobacillus considerably increased,whereas the proportion of genus Leptospirillum and genus Sulfobacillus ***,with the aeration of N_2,the proportion of genus Acidithiobacillus and Leptospirillum increased,but genus Sulfobacillus *** there was no aeration,the microbial community was similar to the inocula with the proportion of genus Leptospirillum *** results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO_2 and N_2 was favourable for the growth of sulfur-oxidizer(***) and iron-oxidizer(***) respectively,which could be used for the regulation of microorganisms' role in mineral bioleaching.

关键词： bioleaching CO2 N2 chalcocite pyrite microbial community

Identification of potential electrotrophic microbial community in paddy soils by enrichment of microbial electrolysis cell biocathodes

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2020年第1期32卷 411-420页

作者： Xiaomin Li Longjun Ding Haiyan Yuan Xiaoming Li Yongguan Zhu State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesShuangqing RoadNo.18Haidian DistrictBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Key Lab of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesJimei RoadNo.1799Jimei DistrictXiamen 361021China

Electrotrophs are microbes that can receive electrons directly from cathode in a microbial electrolysis cell(MEC).They not only participate in organic biosynthesis,but also be crucial in cathode-based ***,little is known about the electrotrophic community in paddy ***,the putative electrotrophs were enriched by cathodes of MECs constructed from five paddy soils with various properties using bicarbonate as an electron acceptor,and identified by 16S rRNA-gene based Illumina *** electrons were gradually consumed on the cathodes,and 25%–45% of which were recovered to reduce bicarbonate to acetic acid during MEC *** was the dominant bacterial phylum on the cathodes,and Bacillus genus within this phylum was greatly enriched and was the most abundant population among the detected putative electrotrophs for almost all ***,several other members of Firmicutes and Proteobacteria may also participate in electrotrophic process in different *** pH,amorphous iron and electrical conductivity significantly influenced the putative electrotrophic bacterial community,which explained about 33.5% of the community structural *** study provides a basis for understanding the microbial diversity of putative electrotrophs in paddy soils,and highlights the importance of soil properties in shaping the community of putative electrotrophs.

关键词： Electrotroph Paddy soil microbial electrolysis cell microbial community Soil property

Revealing the anaerobic acclimation of microbial community in a membrane bioreactor for coking wastewater treatment by Illumina Miseq sequencing

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2018年第2期30卷 139-148页

作者： Jiadi Zhu Lujun Chen Yan Zhang Xiaobiao Zhu School of environment Tsinghua Uniuersity Beijing 100084 China Zhejiang Provincial Key Laboratory of Water Science and Technology Department of Environmental Technology and Ecology Yangtze Delta Region Institute of Tsinghua University Zhejiang Jiaxing 314050 China School of Environmental and Civil Engineering Jiangnan University Wuxi 214222 China College of Chemical Engineering Beijing University of Chemical Technology Beijing 200029 China

The dynamic change of microbial community during sludge acclimation from aerobic to anaerobic in a MBR for coking wastewater treatment was revealed by Illumina Miseq sequencing in this study. The diversity of both Bacteria and Archaea showed an increase–decrease trajectory during acclimation, and exhibited the highest at the domestication interim. Ignavibacteria changed from a tiny minority（less than 1%） to the dominant bacterial group（54.0%） along with acclimation. The relative abundance of Betaproteobacteria kept relatively steady, as in this class some species increased coupled with some other species decreased during acclimation. The dominant Archaea shifted from Halobacteria in initial aerobic sludge to Methanobacteria in the acclimated anaerobic sludge. The dominant bacterial and archaeal groups in different acclimation stages were indigenous microorganisms in the initial sludge, though some of them were very rare. This study supported that the species in＂rare biosphere＂ might eventually become dominant in response to environmental change.

关键词： microbial community Acclimation Coking wastewater Anaerobic MBR Miseq sequencing

Bioleaching of Pb-Zn-Sn chalcopyrite concentrate in tank bioreactor and microbial community succession analysis

在线全文

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2013年第12期23卷 3758-3762页

作者：王军赵红波庄田覃文庆朱珊邱冠周中南大学资源加工与生物工程学院长沙410083 中南大学生物冶金教育部重点实验室长沙410083

The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite.

关键词： chalcopyrite tank bioleaching microbial community PCR-RFLP technique

Bamboo charcoal addition enhanced the nitrogen removal of anammox granular sludge with COD:Performance,physicochemical characteristics and microbial community

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2022年第5期34卷 55-64页

作者： Menglei Guo Ying Jiang Junxiang Xie Qianfei Cao Qun Zhang Adams Mabruk Chongjun Chen School of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou 215009China Jiangsu Provincial Key Laboratory of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China

The effects of different chemical oxygen demand(COD)concentrations on the anammox granular sludge with Bamboo Charcoal(BC)addition were evaluated in UASB *** results showed that the average total nitrogen(TN)removal efficiency was reduced from 85.9%to 81.4%when COD concentration was increased from 50 to 150 mg/***,the TN removal efficiency of BC addition reactors was dramatically 3.1%-6.4%higher than that without BC under different COD *** average diameter of granular sludge was 0.13 mm higher than that without *** settling velocity was increased by elevated COD concentration,while the EPS and VSS/SS were increased with BC *** high-throughput Miseq sequencing analyses revealed that the bacterial diversity and richness were decreased under COD addition,and the Planctomycetes related to anammox bacteria were Candidatus Brocadia and Candidatus *** Metagenomic sequencing indicated that the abundance of denitrification related functional genes all increased with elevated COD,while the abundance of anammox related functional genes of *** func-tional genes related to anammox was hydrazine synthase encoding genes(hzsA,hzsB and hzsB).The average relative abundance of hzs genes in the reactor with BC addition was higher than the control at COD concentrations of 50 mg/L and 150 mg/*** functional genes of denitrification mediated by BC were higher than those without BC throughout the operation *** is interesting to note that BC addition greatly enriched the related func-tional genes of denitrification and anammox.

关键词： Anammox granular sludge Bamboo charcoal COD concentration Nitrogen removal microbial community

Changes in soil microbial community structure associated with two types of genetically engineered plants analyzing by PLFA

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2005年第1期17卷 130-134页

作者： XUEKai LUOHai-feng QIHong-yan ZHANGHong-xun DepartmentofEnvironmentalBioteehnology ResearchCenterforEco-EnvironmentalSciencesChineseAcademyofSciencesBeijing100085China

With the rapid expansion of GEPs(genetically engineered plants), people are more and more concerned about the ecological risks brought by their release. Assessing the effect of GEPs on soil microbial ecology is indispensable to study their ecological risks. In our study, the phospholipids fatty acid(PLFA) method was used to analyze the microbial community of soil samples collected from fields with two types of GEPs_Bt transgenic corn and PVY(potato virus Y) cell protein gene transgenic potato. The principal components analysis(PCA) showed all controls were on the right of related GEPs samples along the PC1 (the first principal component) axis, which means a decrease of fungi in soils with genetically engineered crop since most of PLFAs that are strongly positively correlated with PC1 represent fungi. For samples collected from Bt transgenic cornfield, the ratios of gram_positive to gram_negative bacteria were less than those of controls. For samples of transgenic potato field, these ratios were lower than those of controls when soils were collected from deep layer(20—40 cm), but were higher when soils collected from surface layer(0—20 cm). For soils collected from 0—20 cm, the ratios of fungi to bacteria for all GEPs samples were at the same level. So were such rations for all controls. Changes of soil microbial community in two types of GEPs fields were detected in our study, but the causes and more information still needs further study.

关键词： GEPs microbial community PCA PLFA GC_MS