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Isolation,characterization and extraction of mer gene of Hg^(2+) resisting strain D_2

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Transactions of Nonferrous Metals Society of China 2010年第3期20卷 507-512页

作者：曾晓希汤建新蒋佩刘宏伟戴志敏刘学端 School of Mineral Processing and Bioengineering Central South University Hunan Key Laboratory of Green Packaging and Biological Nanotechnology Hunan University of Technology

mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence,growth,minimal inhibitory concentrations(MICs) of metals,antibiotic susceptibility and mer gene were conducted.The strain D2 can grow in the medium containing 60 mg/L mercury ion.It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa,and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance.The mer RT gene sequence was amplified from chromosome.Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.

关键词：基因分离汞污染 mer 基因提取鉴定 rDNA序列抗生素敏感性 rRNA基因

Time course degeneration and expression of glial fibrillary acidic protein in mer-knockout mice

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Chinese Medical Journal 2010年第7期123卷 949-953页

作者： LIANG Xiao-ying WANG Huai-zhou WANG Ning-li Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Visual Sciences Key Laboratory Beijing 100730 China Department of Ophthalmology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Visual Sciences Key Laboratory Beijing 100730 China Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Visual Sciences Key Laboratory Beijing 100730 China

Background Muller cells in the mammalian retina normally express low levels of glial fibrillary acidic protein （GFAP）; however, its expression is upregulated in response to the loss of retinal neurons. The change in expression of GFAP is one of the earliest indicators of retinal damage and is correlated with the time course of disease. The aim of this study was to investigate the time course of degeneration and the expression of GFAP in the retina of mer knockout mice. Methods A total of 30 mer knockout mice, aged from 15-20 days to 1 year and 32 age-matched wild type mice as controls were tested. Immunohistochemistry was used to show the expression of GFAP in the central and peripheral retina of mer knockout and control mice at postnatal age of 15 days （P15d）, 20 days （P20d）, 4 weeks （P4w）, 6 weeks （P6w）, 8 weeks （P8w）, 3 months （P3m）, 6 months （P6m） and 1 years （P1y）.Results The expression of GFAP in the central and peripheral retina of wild type mice was limited to the retinal ganglion cell and nerve fiber layers. In the central retina of mer knockout mice, GFAP expression was upregulated at P4w and GFAP immunolabelling penetrates across the entire thickness of the retina at P8w; whereas in the peripheral retina, the GFAP expression was upregulated at P20d and GFAP immunolabelling penetrates the entire retina after P4w. Conclusions Increased expression of GFAP in Muller cells of mer knockout mice occur at P20d in the peripheral retina and P4w in the central retina. GFAP expression in Muller cells appears to be a secondary response to the loss of retinal neurons. Increased expression of GFAP may occur prior to any detectable morphological changes in the retina. This study suggests that the loss of retinal neurons may begin in the early stages of retinitis pigmentosa, prior to the discovery of any morphological changes in the retina.

关键词： glial fibrillary acidic protein retinal degeneration mer retinitis pigmentosa

TAM受体在类风湿关节炎患者血清中表达的水平及临床意义

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TAM受体在类风湿关节炎患者血清中表达的水平及临床意义

作者：张红苏州大学

学位级别：硕士

目的:通过检测类风湿关节炎(Rheumatoid Arthritis,RA)患者血清中可溶性(soluble,s)TAM(Tyro 3-Axl-mer的简称)受体酪氨酸激酶的水平,初步分析TAM受体在RA患者血清中的表达水平及其与RA患者的临床、实验室指标和疾病活动性的关系。进一... 详细信息

目的:通过检测类风湿关节炎(Rheumatoid Arthritis,RA)患者血清中可溶性(soluble,s)TAM(Tyro 3-Axl-mer的简称)受体酪氨酸激酶的水平,初步分析TAM受体在RA患者血清中的表达水平及其与RA患者的临床、实验室指标和疾病活动性的关系。进一步探讨TAM受体在RA中的表达水平,以期为RA的诊疗提供新思路。方法:1.收集2020年1月至2021年10月苏州大学附属第一医院门诊或住院的68例RA患者外周血清以及同时期苏州大学附属第一医院体检中心30名健康体检志愿者的外周血清,将其分为RA组和健康对照组(healthycontrol,HC组);同时收集RA患者的临床资料,如性别、年龄、关节疼痛数(tenderjointcount,TJC)、关节肿胀数(swollen joint count,SJC),晨僵时间,红细胞沉降率(erythrocyte sedimentation rate,ESR)、C-反应蛋白(C-reactive protein,CRP)、类风湿因子(rheumatoid factor,RF)及抗环瓜氨酸肽抗体(Anti-Cyclic citrullinated peptideantibody,抗CCP抗体)水平,并计算每位RA患者的28个关节疾病活动度评分(Disease activity score,DAS28-ESR 评分),按照 DAS28 评分将 RA 患者分为疾病缓解组(DAS28mer 受体水平。3.比较RA患者与健康对照组血清sTAM受体表达水平;比较RA患者疾病缓解组与疾病活动组的sTyro 3、sAxl和smer受体水平;分析sTyro 3、sAxl和smer受体水平与RA患者临床及实验室指标的关系;比较治疗三个月前后RA患者的临床和实验室指标以及sTAM受体水平的变化。结果:1.RA组患者血清sTyro 3受体水平显著低于健康对照组(6.02±2.10ug/L vs.9.68±2.00ug/L,P0.05)。继续治疗3个月后,随着患者病情缓解,RA患者中sTyro3受体水平较前显著升高(4.94ug/L vs.6.91ug/L,P0.05)。继续治疗3个月后,随着患者病情缓解,sAxl受体水平较前显著升高(54.03ng/L vs.107.65ng/L,P=0.001)。3.RA组患者血清smer受体水平(8.66±3.04ug/L)显著低于健康对照组(16.70±4.65ug/L,Pmer受体水平显著低于疾病缓解组(7.01 ug/L vs.11.12ug/L,Z=-2.702,P=0.007)。RA 患者血清 smer 受体水平与TJC(r=-0.240,P=0.049)、SJC(r=-0.306,P=0.011)、晨僵时间(r=-0.427,P<0.001)、DAS28 评分(r=-0.373,P=0.002)、ESR(r=-0.329,P=0.006)及 CRP(r=-0.330,P=0.00

关键词： Tyro 3 Axl mer 类风湿关节炎

mer在Jurkat细胞中的表达和抗凋亡作用

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癌症 2007年第7期26卷 698-702页

作者：范磊戴兰沈飞周梦云董宁征阮长耿苏州大学附属第一医院江苏省血液研究所江苏苏州215006

背景与目的:mer是Axl受体酪氨酸激酶家族中的一员,其配体Gas6和mer结合后可以激活mer的酪氨酸激酶活性以及下游信号转导途径,在细胞炎症、凋亡等方面发挥作用。近年来对于mer及其家族成员功能的研究不断完善,但关于其在恶性疾病方面的... 详细信息

背景与目的:mer是Axl受体酪氨酸激酶家族中的一员,其配体Gas6和mer结合后可以激活mer的酪氨酸激酶活性以及下游信号转导途径,在细胞炎症、凋亡等方面发挥作用。近年来对于mer及其家族成员功能的研究不断完善,但关于其在恶性疾病方面的研究报道甚少。本实验探讨mer在恶性T淋巴细胞白血病细胞株Jurkat中的表达及其对Jurkat细胞凋亡过程的影响,并对其抗凋亡机制进行初步探究。方法:利用流式细胞仪分别检测正常外周血、骨髓中T淋巴细胞以及Jurkat细胞中mer的表达。利用RNAi技术敲除Jurkat细胞中mer的表达后采用噻唑蓝(MTT)法检测mer对于Jurkat细胞增殖的影响,AnnexinV/PI双染流式细胞术检测mer对于Jurkat细胞血清撤离诱导凋亡过程的影响。最后利用荧光定量PCR检测RNA干扰后凋亡相关基因Bcl-2和Caspase-3以探讨mer在Jurkat细胞中抗凋亡机制。结果:mer在正常外周血和骨髓的T细胞中均未见表达,而在Jurkat细胞中高表达(51.1%)。特异性的siRNA(第1403位点)可以抑制86.0%mer在Jurkat细胞中的表达。在血清撤离48h导致的凋亡实验中正常Jurkat细胞仅有1.5%的凋亡率,而敲除mer后的Jurkat细胞凋亡率达到15.3%,而MTT增殖实验中阻断mer前后差异无统计学意义(P>0.05)。定量PCR结果显示敲除mer基因后Jurkat细胞中Bcl-2明显下调,为对照组的42.7%,而Caspase-3变化不显著。结论:mer在Jurkat细胞中异常高表达,并可能通过Bcl-2途径影响细胞凋亡过程。

关键词： mer 受体酪氨酸激酶白血病 Jurkat细胞凋亡 Bcl-2

体积小巧声音大气 KEF R5 Meta落地式音箱

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视听前线 2023年第9期 47-50页

作者：家祺小路(图) 不详

KEF R Meta在今年初系列发布后迅速获大量好评,受到广大媒体和消费者的肯定。除了各大专业媒体的满分评价,在6月份日本最大规模影音产品奖项VGP2023 HKEF SUMmer上,RMeta系列就获得了最优秀的特别大赏,其中R11 Meta还获得了评审特别赏。... 详细信息

KEF R Meta在今年初系列发布后迅速获大量好评,受到广大媒体和消费者的肯定。除了各大专业媒体的满分评价,在6月份日本最大规模影音产品奖项VGP2023 HKEF SUMmer上,RMeta系列就获得了最优秀的特别大赏,其中R11 Meta还获得了评审特别赏。而8月刚刚公布的美国最权威的EISAHi-Fi音响奖同样将RMeta系列选为获奖产品。

关键词：落地式音箱影音产品 KEF 获奖产品 mer R11 媒体

Role of Tyro3, Axl, and mer Receptors and Their Ligands (Gas6, and Protein S) in Patients with Hepatocellular Carcinoma

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Journal of Cancer Therapy 2017年第2期8卷 112-130页

作者： Soichiro Uehara Yuichi Fukuzawa Tomohiko Matuyama Katuhiro Gotoh Department of Gastroenterology Hizirigaoka Hospital Date Japan

The Tyro3, Axl and mer (TAM) receptor tyrosine kinases are activated by endogenous ligands, protein S1 (PROS1) and growth arrest-specific gene 6 (Gas6), and those have important effects on cell biology. These receptors (Rs) can be shad from the cell membrane and their soluble(s) forms can be found in plasma. We investigated the fluctuation and interactive role of sTAMRs and its ligands in patients with hepatocellular carcinoma (HCC), hepatitis groups, and healthy normal adult controls (NC). The measurement cases were 45 patients with HCC group (stage 1 in 4, stage 2 in 8, stage 3 in 16, and stage 4 in 17), 4 patients with fulminant hepatitis (FH), 14 patients with acute hepatitis (AH), 10 patients with chronic hepatitis (CH), 16 patients with liver cirrhosis (LC), and 20 NCs matched by age. Plasma levels of three sTAMRs and their ligands were measured by ELISA. In comparison with NCs, Gas6, des-γ-carboxy Gas6, and sTAMRs levels were significantly higher in HCC patients, but free PROS1 levels were significantly lower. The sTyro3 and sAxl levels peaked HCC stages 2 and 3 respectively, and gradually decreased afterwards while maintaining high levels. smer levels increased with the progression of HCC. Gas6 and des-γ-carboxy Gas6 levels gradually increased, and PROS1 levels decreased with the progression of HCC. Gas6 levels were positively correlated with sAxl levels, whereas smer levels were negatively correlated with free PROS1 levels. sTAMRs and Gas6 levels increased in parallel to the progression of HCC fibrosis. Through the progression of HCC, Axl played the major role in TAMRs activation. However, sTYro3 continued increasing rapidly from the early stage, and that of mer increased throughout the progression. Roles of Axl may be changed in mer, because des-γ-carboxy Gas6 levels increasing with Gas6 in the advanced stage of HCC cannot send a signal to Axl.

关键词： Tyro3 Axl mer Gas6 Protein S Hepatocellular Carcinoma

TYRO3^(-/-)AXL^(-/-)mer^(-/-)小鼠骨髓细胞mRNA的差异表达谱

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基因组学与应用生物学 2016年第4期35卷 832-837页

作者：唐红梅贾长虹赵丽娜丁存宝华北理工大学生命科学学院唐山063000

TYRO3、AXL和mer受体是受体酪氨酸激酶亚家族之一,广泛地表达在哺乳动物神经、免疫、生殖、血液等系统多种细胞中,促进细胞存活、增殖和分化。本研究利用基因芯片技术筛选基因敲除的TYRO3^(^(-/-))AXL^(-/-)mer^(-/-)小鼠骨髓细胞中差... 详细信息

TYRO3、AXL和mer受体是受体酪氨酸激酶亚家族之一,广泛地表达在哺乳动物神经、免疫、生殖、血液等系统多种细胞中,促进细胞存活、增殖和分化。本研究利用基因芯片技术筛选基因敲除的TYRO3^(^(-/-))AXL^(-/-)mer^(-/-)小鼠骨髓细胞中差异性表达的m RNA,并进行生物信息学分析。与正常小鼠骨髓细胞相比,TYRO3^(-/-)AXL^(-/-)mer^(-/-)小鼠骨髓细胞中差异表达基因探针共1 363条,其中表达上调的基因探针499条、下调的基因探针864条。GO功能富集分析发现上调的基因主要参与免疫反应,下调的基因主要参与造血。KEGG Pathway富集分析发现上调的基因主要参与细胞粘附分子和抗原呈递等信号通路。Real-time PCR验证5个差异基因,与芯片中表达变化趋势一致。因此TYRO3、AXL和mer受体共同参与调控机体的免疫反应和血细胞分化。

关键词： AXL mer TYRO3 基因敲除骨髓细胞

CATALOGO SISTEMATICO, ZOOGEOGRAFICO E GEONEMICO DEI LACERTIDAE DI CORSICA, ITALIA E ISOLE MALTESI

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Natural Disaster Reduction in China 1982年第1期 39-95页

作者： SILVIO BRUNO Centro Studi Ecologici Appeninici Parco Nazionale d’Abruzzo

INTRODUZIONE I tassonomi 《mertensiani》 hanno descritto -- anche per la Corsica, le Isole Maltesie l’Italia -- un elevato numero di Lacertidae che riconoscono come sottospecie. In mol-ti casi, tuttavia, il termine 《sottospecie》 e stato ed e erroneamente sostituito a quello di《varieta》 nel suo significato di 《razza geografica》. Se, per esempio, un’esauriente ana-lisi biometrico-morfologica mette in luce una differenza media tra i campioni di Lacer-

关键词： La PIO Pi CATALOGO SISTEMATICO ITALIA E ISOLE MALTESI ZOOGEOGRAFICO E GEONEMICO DEI LACERTIDAE DI CORSICA Pro DEI der LER mer DI

Detecting emboli from Doppler ultrasound signals with the wavelet packet analysis method

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Chinese Journal of Acoustics 2004年第3期23卷 244-250页

作者： CHENXi SUNZhimin WANGYuanyuan WANGWeiqi DepartmentofElectronicEngineering FudanUniversityShanghai200433

A Doppler ultrasound analysis method based on wavelet package transform was proposed for embolic detection. The embolic Doppler signal was firstly decomposed using the wavelet packet. Then the sensitive characteristics were calculated from each sub-band signal and used in the emboli classification. This method was applied to analyze 300 cases simulated and 163 cases clinical Doppler signals. The minimum error ratio of embolic detection using this method was 13 percents lower than that using the traditional spectrogram analysis method. It was shown that this method overcame the limit between the time and frequency resolution in the short time Fourier transform, improved the accuracy of embolic detection greatly and extracted more reliable parameters for the clinical diagnosis.

关键词： Detecting emboli from Doppler ultrasound signals with the wavelet packet analysis method mer