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加味柴胡疏肝汤联合奥卡西平片治疗卒中后癫痫患者的疗效及血清S100B、mbp水平分析

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辽宁医学杂志 2024年第1期38卷 80-83页

作者：王文龙漯河市中心医院漯河市第一人民医院河南漯河462005

目的探究加味柴胡疏肝汤联合奥卡西平片治疗卒中后癫痫患者的疗效及血清S100B、mbp水平分析。方法采用随机分组法将我院2019年10月到2021年10月重症医学及神经重症科收治的76例患者分为观察组与对照组,两组各例38例。对照组采用奥卡西... 详细信息

目的探究加味柴胡疏肝汤联合奥卡西平片治疗卒中后癫痫患者的疗效及血清S100B、mbp水平分析。方法采用随机分组法将我院2019年10月到2021年10月重症医学及神经重症科收治的76例患者分为观察组与对照组,两组各例38例。对照组采用奥卡西平片治疗,观察组在此基础上联合使用加味柴胡疏肝汤治疗。观察两组患者临床疗效与不良反应发生率,比较两组患者治疗前后血清中S100B、mbp、NSE的水平变化以及NIHSS评分。结果两组患者治疗后观察组总有效率为94.74%显著高于对照组78.96%(χ^(2)=4.145,P=0.041)。两组患者治疗前S100B、NSE、mbp水平无统计学差异(P>0.05)。治疗后观察组S100B、NSE、mbp水平较对照组显著降低(P0.05)。治疗后观察组NIHSS评分较对照组显著降低(Pmbp水平、降低不良反应发生率,修复患者神经损伤,提高生活质量。

关键词：加味柴胡疏肝汤奥卡西平片癫痫 S100B mbp

Evaluation of a Rat Model of Experimental Autoimmune Encephalomyelitis with Human mbp as Antigen

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Cellular & Molecular Immunology 2004年第5期1卷 387-391页

作者： LinGuo YuehuaLi HongyiLin XiaohuiJi JingLi LingliQue YingdongZhang YushanRong JianwenWang DepartmentofPathophysiology NanjingMedicalUniversityNanjing210029China. DepartmentofImmunology NanjingMedicalUniversityNanjing210029China. DepartmentofNeurology NanjingBrainHospital.Nanjing210029China. TheCentreofMedicolegalExpertise PoliceBureau.Nanjing210000China. DepartmentofForensicMedicine NanjingMedicalUniversityNanjing210029China.

Experimental autoimmune encephalomyelitis(EAE) is a good model for human multiple sclerosis(MS) research.However,there are some defects in the traditional models.Here,we improved the model by using the human myelin basic protein(mbp) as antigen.EAE was induced by immunization of female Wistar rats with human mbp.Compared with the traditional models,the new model was evaluated by clinical signs to pathological changes.The immune state of the model was assessed by the lymphocyte infiltrative response and levels of TNF-α,IFN-γ,IL-10.It was found that most of rats exhibited tail tone loss and hind-limb paralysis, also there were demyelination,infiltrative lymphocyte foci,“neuronophagia”in the cortex of cerebra and the white matter of spinal cords.PBMCs and spleen lymphocytes were strongly responsive to the stimulation of mbp and PHA.The levels of TNF-α and IFN-γ were altered with the severity of EAE.In the remitting phase, IL-10 was increased significantly.This study demonstrates that the animal model of EAE induced by human mbp bears resemblance to the features of human multiple sclerosis and promises to be a better model than ever before for the study of MS.Cellular & Molecular Immunology.2004;1(5):387-391.

关键词： EAE model MS mbp

Effects of Minimally Invasive Puncture and Drainage of Intracranial Hematoma on the Blood-brain Barrier in Patients with Cerebral Hemorrhage

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第1期27卷 101-103页

作者：黄晓江郭守刚王伟 Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

The effects of minimally invasive surgery on the blood-brain barrier （BBB） of 30 patients with cerebral hemorrhage were investigated. Difference of the BBB index and serum mbp concentration were assessed in 15 cases of conservative treatment group and 15 cases of minimally invasive surgery group. The BBB index in minimally invasive surgery group was significantly lower than in conservative treatment group （P〈0.05）, and the BBB index in the two treatment groups was significantly higher than in control group （P〈0.01）. Serum mbp concentration in minimally invasive surgery group was significantly lower than in conservative treatment group （P〈0.05）, and that in the two treatment groups was significantly higher than in control group （P〈0.01）. It was suggested the permeability of BBB in patients with cerebral hemorrhage was increased, and BBB index and serum mbp concentration in patients with cerebral hemorrhage were increased. Minimally invasive surgery can reduce the lesion of cytotoxicity to BBB and cerebral edema.

关键词： intracerebral hemorrhage blood-brain barrier BBB index mbp

Action of Schwann Cells Implanted in Cerebral Hemorrhage Lesion

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Biomedical and Environmental Sciences 2007年第1期20卷 47-51页

作者： HONG WAN SHAO-DONG ZHANG JUN-HUA LI Beijing Neurosurgical Institute Capital Medical University Beijing 100050 China

Objective To investigate whether there is neogenesis of myelin sheath and neuron after transplantation of Schwann cells into cerebral hemorrhage lesion. Methods Schwann cells were expanded, labeled with BrdU in vitro and transplanted into rat cerebral hemorrhage with blood extracted from femoral artery and then injected into the basal nuclei. Double immunohistochemistry staining and electron microscopy were used to detect the expression of BrdU/mbp and BrdU/GAP-43 and remyelination. Results BrdU/mbp double positive cells could be seen at 1 week up to 16 weeks after transplantation of Schwann cells. Thin remyelination was observed under electron microscope. GAP-43 positive cells appeared after 12 weeks and were found more in Hippocamp. Conclusions Grafted Schwann cells participate in remyelination and promoter nerve restore in rat cerebral hemorrhage.

关键词： Schwann cell mbp GAP-43

Visible and near-infrared luminescence of polycatenated cationic pyridinone lanthanide networks

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Science China Chemistry 2016年第4期59卷 436-441页

作者： Yubo Shu Weisheng Liu Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province State Key Laboratory of Applied Organic ChemistryCollege of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

A series of isomorphic lanthanide coordination polymers [Ln(III)(mbp)_2(NO_3)_2(Br)?2C_3H_6O] [Ln=Eu, Tb, Er, Yb, and Gd; mbp=N,N′-methylene-bis(pyridin-4-one)] featuring polycatenated sql cationic network and incorporated bromide counter ion were prepared. Their visible and near-infrared(NIR) luminescence properties were characterized by steady-state excitation and emission spectra, as well as luminescence lifetimes and quantum yields. The D_(2d) dodecahedron coordination geometry causes visible light excitations and strongly monochromatic emissions. The external heavy-atom environment induces remarkable enhancement on the NIR emissions. The sensitization processes are revealed by analyzing the electronic properties of mbp ligand.

关键词：近红外发光稀土阳离子网络网络可见光吡啶酮 Ln(Ⅲ) 配位聚合物 mbp

The Evolution of Water Property in the Mackenzie Bay Polynya During Antarctic Winter

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Journal of Ocean University of China 2017年第5期16卷 766-774页

作者： XU Zhixin GAO Guoping XU Jianping SHI Maochong College of Oceanic and Atmospheric Sciences Ocean University of China Qingdao 266100 P. R. China State Key Laboratory of Satellite Ocean Environment Dynamics (SOED) State Oceanic Administration Hangzhou 310012 P. R. China Shanghai Ocean University College of Marine Sciences Shanghai 201306 P. R. China The Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 P. R. China

Temperature and salinity profile data, collected by southern elephant seals equipped with autonomous CTD-Satellite Relay Data Loggers(CTD-SRDLs) during the Antarctic wintertime in 2011 and 2012, were used to study the evolution of water property and the resultant formation of the high density water in the Mackenzie Bay polynya(mbp) in front of the Amery Ice Shelf(AIS). In late March the upper 100–200 m layer is characterized by strong halocline and inversion thermocline. The mixed layer keeps deepening up to 250 m by mid-April with potential temperature remaining nearly the surface freezing point and sea surface salinity increasing from 34.00 to 34.21. From then on until mid-May, the whole water column stays isothermally at about^(-1).90℃while the surface salinity increases by a further 0.23. Hereafter the temperature increases while salinity decreases along with the increasing depth both by 0.1 order of magnitude vertically. The upper ocean heat content ranging from 120.5 to 2.9 MJ m^(-2), heat flux with the values of 9.8–287.0 W m^(-2) loss and the sea ice growth rates of 4.3–11.7 cm d^(-1) were estimated by using simple 1-D heat and salt budget methods. The mbp exists throughout the whole Antarctic winter(March to October) due to the air-sea-ice interaction, with an average size of about 5.0×10~3 km^2. It can be speculated that the decrease of the salinity of the upper ocean may occur after October each year. The recurring sea-ice production and the associated brine rejection process increase the salinity of the water column in the mbp progressively, resulting in, eventually, the formation of a large body of high density water.

关键词：冰间湖水柱冬季南极演变性质数据记录器 mbp

Repeated exposure to moxa-burning smoke: its acute and chronic toxicities in rats

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Journal of Traditional Chinese Medicine 2018年第1期38卷 67-75页

作者： Han Li Liu Changxi Liu Ping Hu Hai Yang Jia Cai Hong Lim Minyee Zhu Maoxiang Pan Xiujie Huang Jian Cui Yingxue Lao Lixing Zhao Baixiao Shool of Chinese medicine Beijing University of Chinese Medicine Healthcare Management Center Beijing Electric Power Hospital Department of Acupunture Beijing Hospital of Acupuncture and Moxibustion Shool of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine Department of Traditional Chinese Medicine Chongqing Yubei District Hospital of Traditional Chinese Medicine Institute of Radiation Medicine China Academy of Military Medical Science Department of Acupunture Beijing Dong Fang Hospital Department of Acupunture Beijing Hospital of Traditional Chinese Medicine School of Chinese Medicine The University of Hong Kong

OBJECTIVE: To assess toxicities of the air in Chi-nese medicine clinics polluted by moxa-burning smoke due to moxibustion-derived burning products(mbp).METHODS: Both acute and chronic toxicity studies were conducted. For the acute toxicity study, five groups of Wistar rats(n = 16/group, male: female =1∶1) were exposed to five different concentrations(95%, 90%, 85%, 80% and 75%, respectively) of mbp for 2 h. For the chronic toxicity study, another three groups of male rats(n = 21/group) were exposed to mbp in three concentrations(10%, 40%and 70%, respectively) and one control group exposed to clean air 20 min/d for 144 d. Routine examinations were performed and analyzed by analysis of variance and dose-response relationship.RESULTS: In the acute toxicity study, the number of dead rats in the 95%, 90%, 85%, 80% and 75%groups were 16, 13, 7, 6 and 3, respectively, with LD50 of 86.274% after or during the 2 h exposure.In the chronic toxicity study, mbp exposure induced a decline in activity of the rats. Rats in the10% group showed no signs of toxicity, while those in the 40% mbp group showed toxicity effects on the body weights(P mbp group also presented with reversible damage in the blood coagulation system(P mbp, which is equivalent to 27.45 mg/m3, was under the critical threshold for male rats' safety. Exposure to mbp above that limit induced lung damage. mbp in clinics need to be reduced to a safe level with enhanced ventilation.

关键词：毒性老鼠 Wistar mbp 剂量反应安全水平学习 sis

芪枝渴痹通方对糖尿病小鼠坐骨神经自噬相关蛋白的影响

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中华中医药杂志 2022年第5期37卷 2870-2874页

作者：赵芸芸王萌萌王国强仝小林王秀阁长春中医药大学长春130117 长春中医药大学附属医院长春130021 中国中医科学院广安门医院北京100053 长春中医药大学附属医院内分泌代谢病科

目的:探讨芪枝渴痹通方对糖尿病小鼠坐骨神经自噬相关蛋白的影响。方法:C57BL小鼠单次腹腔注射120 mg/kg STZ建立糖尿病模型。选取造模成功的50只糖尿病小鼠随机分为模型组,芪枝渴痹通方低、中、高剂量组及甲钴胺组,每组10只,设立空白组... 详细信息

目的:探讨芪枝渴痹通方对糖尿病小鼠坐骨神经自噬相关蛋白的影响。方法:C57BL小鼠单次腹腔注射120 mg/kg STZ建立糖尿病模型。选取造模成功的50只糖尿病小鼠随机分为模型组,芪枝渴痹通方低、中、高剂量组及甲钴胺组,每组10只,设立空白组(n=10)。芪枝渴痹通方低、中、高剂量组及甲钴胺组在糖尿病小鼠成模后给予芪枝渴痹通方(7.38、14.76、29.52 g·kg^(-1)·d^(-1))及甲钴胺组(0.189 g·kg^(-1)·d^(-1))灌胃,空白组与模型组给予等体积蒸馏水灌胃。免疫组化法检测糖尿病小鼠坐骨神经P0、mbp蛋白表达,荧光定量PCR法及Western blot法检测LC3、P62、Beclin^(-1)、ULK1 mRNA及蛋白表达。结果:与空白组比较,模型组小鼠坐骨神经组织P0、mbp蛋白表达显著降低(Pmbp蛋白表达显著升高(P<0.01)。与空白组比较,模型组小鼠坐骨神经组织LC3、Beclin^(-1)、ULK1 mRNA表达显著降低(P<0.01,P<0.05),LC3、Beclin^(-1)蛋白表达显著降低(P<0.01,P<0.05),P62 mRNA及蛋白表达升高(P<0.01);与模型组比较,芪枝渴痹通方各剂量组及甲钴胺组LC3、Beclin^(-1)、ULK1 mRNA及蛋白表达显著升高(P<0.01,P<0.05),P62 mRNA及蛋白表达显著降低(P<0.01)。结论:QZKBTF通过激活自噬,对糖尿病小鼠坐骨神经具有保护作用。

关键词：糖尿病周围神经病变自噬芪枝渴痹通方 mbp P0 LC3 P62 Beclin-1

甘氨酸对mbp暴露诱导的猪卵母细胞发育异常的调控机制研究

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甘氨酸对MBP暴露诱导的猪卵母细胞发育异常的调控机制研究

作者：高乐鹏吉林农业大学

学位级别：硕士

高质量配子的产生是家畜繁殖和种质资源保护的前提,环境中塑化剂暴露是引起卵母细胞体外发生的瓶颈-减数分裂异常的高风险因素之一。目前检测到血清和卵泡液中含量最高的塑化剂代谢产物为邻苯二甲酸单丁酯(Mibutyl phthalate,mbp),其结... 详细信息

高质量配子的产生是家畜繁殖和种质资源保护的前提,环境中塑化剂暴露是引起卵母细胞体外发生的瓶颈-减数分裂异常的高风险因素之一。目前检测到血清和卵泡液中含量最高的塑化剂代谢产物为邻苯二甲酸单丁酯(Mibutyl phthalate,mbp),其结构稳定,可在生物体内持续积累,并对人类和动物的生殖系统具有潜在的危害,因此研究mbp暴露对卵母细胞发育异常的机制及寻找改善方法十分必要。前期研究发现,外源补充甘氨酸可以显著提高卵母细胞体外成熟(IVM)和体外受精(IVF)效率。本研究旨在探究甘氨酸是否能改善mbp暴露诱导的卵母细胞发育异常并明确其机制。主要研究结果如下:(1)mbp在母猪血清和卵泡液中残留情况检测。在规模化的3个饲养场收集的母猪血清和卵泡液样品,每厂5个样品,通过ELISA试剂盒检测均检出mbp成分且显著高于对照组组,这表明母猪在生长过程中存在塑化剂持续暴露的风险。(2)探究mbp暴露对猪卵母细胞成熟的影响。构建mbp体外暴露模型(50μM、100μM、200μM),与对照组相比,mbp暴露后卵丘扩散直径和卵母细胞成熟率均显著降低,ROS荧光强度显著升高,且呈剂量性损伤。通过半数致死率(LD50)确定mbp体外暴露模型浓度为50μM,随后通过单细胞转录组测序技术(SMART-seq)探究mbp暴露的对卵母细胞分子调控机制的影响。GO功能富集分析表明mbp暴露主要对细胞骨架组织和细胞质微管结构产生影响,从而导致卵母细胞发育受损。(3)甘氨酸对mbp暴露诱导猪卵母细胞发育异常的调控。研究发现,外源补充6m M甘氨酸能够显著改善mbp暴露诱导的卵母细胞卵丘扩散直径和卵母细胞成熟率异常,并显著改善mbp暴露造成的纺锤体和染色体形态异常情况。线粒体-内质网共定位检测结果表明,与对照组相比,mbp暴露后显著增强了线粒体-内质网之间相互作用,并下调互作蛋白MFN1和MFN2表达。对Ca水平检测发现,甘氨酸能显著改善mbp暴露诱导的[Ca]和[Ca]水平异常,下调促细胞凋亡相关基因Bax、Caspase 3、AIFM1的表达,从而减少细胞凋亡。表明甘氨酸可通过调节线粒体-内质网之间相互作用,改善mbp暴露诱导的猪卵母细胞减数分裂异常和凋亡情况。(4)甘氨酸对mbp暴露诱导的早期胚胎发育异常的调控。研究发现甘氨酸能显著改善mbp暴露诱导的MⅡ期卵母细胞H3K36me3和SETD2蛋白表达水平异常,并显著降低DNA断裂标记物γH2AX表达水平,进一步显著改善由mbp暴露诱导的IVF卵裂率、囊胚率和细胞数下降的问题,使早期胚胎发育达到对照组水平。综上所述,甘氨酸可显著改善mbp暴露诱导的猪卵母细胞及早期胚胎发育异常,本研究系统探究mbp暴露对猪卵子质量及胚胎发育能力的影响,并建立缓解其危害的有效技术方法,将为改善塑化剂暴露下对母畜生殖性能的影响提供理论依据。

关键词：猪卵母细胞 mbp 甘氨酸线粒体