检索结果-南通市图书馆

Substrate Stiffness Affects Endothelial Cell Junctions and mapk signaling pathway

Journal of Biosciences and Medicines 2022年第2期10卷 124-135页

作者： Huanli Wang Chao Zhang Xiaomei Han Jin Xu Yue Zhou School of Biomedical Engineering Shanghai Jiao Tong University Shanghai China

Vascular homeostasis is critical for maintaining normal vascular structure and function. Aging is an irreversible trigger of vascular sclerosis, which causes structural and functional damage to blood vessels, leading to severe atherosclerosis. Endothelial cells (ECs) can respond to mechanical stimuli from the extracellular matrix, causing disruption of endothelial barrier function and activating signaling pathways to regulate cellular behavior under pathological conditions. In this paper, we investigated the effect of substrate stiffness on endothelial cell junctions, and the activation of mitogen-activated protein kinase (mapk) signaling pathways. An in vitro stiffness model was established using polyacrylamide hydrogels of 1 kPa, 20 kPa and 100 kPa. By transcriptome analysis, we found that the cell-cell junction, cadherin binding, cytoskeleton and classical signaling pathways such as mapk and Rho GTPase of endothelial cells were regulated by substrate stiffness. The expression of cell junction-related molecules TJP1, TJP2, JAM3 and JCAD was also found to be reduced at higher stiffness. The mapk signaling pathway-related molecules MAP2K3, MAP2K7, MAP3K3, MAP3K6, mapk3, mapk7 were upregulated with increased stiffness. qRT-PCR analysis showed that the gene expression of JCAD was reduced with increased stiffness. Immunofluorescence staining of VE-cadherin indicated that the total fluorescence level of VE-cadherin decreased significantly with increased stiffness, and stiffness impaired the cell-cell junction with increased punctuation and discontinuity. Western blotting analysis confirmed that the protein expression ratio of pp38mapk/p38mapk increased with stiffness. Our research suggested that substrate stiffness played an important role in regulating endothelial cell integrity and mapk signaling pathway.

关键词： Substrate Stiffness Endothelial Cell Cell Junction mapk signaling pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ethylene thiourea exposure induces neurobehavioral toxicity in zebrafish by disrupting axon growth and neuromuscular junctions

引用

Journal of Environmental Sciences 2024年第3期137卷 108-119页

作者： Jingming Wang Zhiquan Yu Yongfeng Wang Yuanyao Chen Lin Xiao Yanjun Zong Qiyuan Feng Lianqi Peng Huiping Zhang Chunyan Liu Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Ethylene thiourea(ETU)converted from ethylene bisdithiocarbamate(EBDC)fungicides has aroused great concern because of its prevalence and harmful effects.Although ETU-induced neurotoxicity has been reported,the potential mechanisms remain unclear.This study provided insights into its neurotoxic effects at environmentally relevant concentrations in zebrafish.Our findings showed that embryonic exposure to ETU decreased the hatch rate and delayed somite development.Furthermore,ETU treatment significantly reduced the dark velocity in the locomotion assay.The upregulated tendency of the mitogen-activated protein kinases(mapk)pathway(mknk1,atf4,mapkapk3)screened by transcriptome analysis implied motor neuron degeneration,which was validated by subsequent morphological observation,as axon length and branches were truncated in the 62.5μg/L ETU group.However,although the rescue experiment with a p38 mapk inhibitor(SB203580)successfully ameliorated axon degeneration,it failed to reverse the locomotion behaviors.Further exploration of transcriptome data revealed the varied expression of presynaptic scaffold protein-related genes(pcloa,pclob,bsna),whose downregulation might impair the neuromuscular junction(NMJ).Therefore,we reasonably suspected that ETU-induced neurobehavioral deficits might result from the combined effects of the mapk pathway and presynaptic proteins.Considering this,we highlighted the necessity to take precautions and early interventions for susceptible ETU-exposed populations.

关键词： Ethylene thiourea(ETU) Behavior Neuromuscular junction mapk signaling pathway Piccolo and bassoon Danio rerio

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of ginsenoside Rg1 on hematopoietic stem cells in treating aplastic anemia in mice via mapk pathway

引用

World Journal of Stem Cells 2024年第5期16卷 591-603页

作者： Jin-Bo Wang Ming-Wei Du Yan Zheng Internal Medicine of Traditional Chinese Medicine Tongde Hospital of Zhejiang ProvinceHangzhou 310012Zhejiang ProvinceChina Institute of Cardiovascular Disease Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Shanghai Key Laboratory of Molecular Medical Mycology Second Affiliated Hospital of Naval Medical UniversityShanghai 200003China Shanghai Key Laboratory of Traditional Chinese Clinical Medicine Shanghai 201203China Department of Hepatic The Xixi Hospital of Hangzhou Affiliated to Zhejiang University of Traditional Chinese MedicineHangzhou 310023Zhejiang ProvinceChina

BACKGROUND Aplastic anemia(AA)presents a significant clinical challenge as a life-threatening condition due to failure to produce essential blood cells,with the current the-rapeutic options being notably limited.AIM To assess the therapeutic potential of ginsenoside Rg1 on AA,specifically its protective effects,while elucidating the mechanism at play.METHODS We employed a model of myelosuppression induced by cyclophosphamide(CTX)in C57 mice,followed by administration of ginsenoside Rg1 over 13 d.The invest-igation included examining the bone marrow,thymus and spleen for pathological changes via hematoxylin-eosin staining.Moreover,orbital blood of mice was collected for blood routine examinations.Flow cytometry was employed to identify the impact of ginsenoside Rg1 on cell apoptosis and cycle in the bone marrow of AA mice.Additionally,the study further evaluated cytokine levels with enzyme-linked immunosorbent assay and analyzed the expression of key proteins in the mapk signaling pathway via western blot.RESULTS Administration of CTX led to significant damage to the bone marrow’s structural integrity and a reduction in hematopoietic cells,establishing a model of AA.Ginsenoside Rg1 successfully reversed hematopoietic dysfunction in AA mice.In comparison to the AA group,ginsenoside Rg1 provided relief by reducing the induction of cell apoptosis and inflammation factors caused by CTX.Furthermore,it helped alleviate the blockade in the cell cycle.Treatment with ginsenoside Rg1 significantly alleviated myelosuppression in mice by inhibiting the mapk signaling pathway.CONCLUSION This study suggested that ginsenoside Rg1 addresses AA by alleviating myelosuppression,primarily through modulating the mapk signaling pathway,which paves the way for a novel therapeutic strategy in treating AA,highlighting the potential of ginsenoside Rg1 as a beneficial intervention.

关键词： Aplastic anemia Ginsenoside Rg1 Myelosuppression mapk signaling pathway Bone marrow Hematopoietic stem cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

DAP inhibits inflammatory response of TNF-α-induced by modulating the mapk signaling pathway in synovial cell

DAP inhibits inflammatory response of TNF-α-induced by modu...

引用

第十三届全国免疫学学术大会

作者： Nanzhen Kuang Jieying Wang Xiaoping Zeng Chan Dai Huimin Deng Youchang Hu Yingyuan Fu Department of Immunology School of Basic Medical Sciences Nanchang University

Daphnetin extracted from Daphne odora Var.marginata contains coumarin compounds, which has a good anti-inflammatory analgesic effect.In this study, we investigated whether Daphnetin can reduce the inflammatory response of TNF-α-induced by inhibiting mapk signaling pathway in synovial cell of CIA rats.Constructing the model of synovial cells from CIA rats induced by TNF-α.The expression of inflammatory cytokines in synovial cells of CIA rats was observed by real-time PCR and ELISA.The expression of mapk signal pathway protein and nuclear translocation was detected by western blot and immunofluorescence assay.The results show, The levels of IL-6, TGF-β, MMP-3 and MMP-13 mRNA in IL-6, TGF-β, MMP-3 and MMP-13 were significantly lower than those in the culture supernatant Model control group;The expression of p38, ERK1/2, JNK protein in synovial cells of CIA rats induced by TNF-α decreased the expression of p-p38, p-ERK1/2 and p-JNK in the nucleus.In conclusion, daphnetin can affect the activation of mapk signaling pathway and reduce the expression of inflammatory factors throgh inhibiting the mapk signaling pathway, play the role of anti-rheumatic inflammation.

关键词： Daphnetin synovial cells mapk signaling pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Electroacupuncture Attenuated Phenotype Transformation of Vascular Smooth Muscle Cells via PI3K/Akt and mapk signaling pathways in Spontaneous Hypertensive Rats

引用

Chinese Journal of Integrative Medicine 2022年第4期28卷 357-365页

作者： CHEN Xin-yu YANG Lu-ping ZHENG Ya-ling LI Yu-xi ZHONG Dong-ling JIN Rong-jiang LI Juan School of Health Preservation and Rehabilitation Chengdu University of Traditional Chinese MedicineChengdu610072China Food Science and Nutrition School University of LeedsLeedsLS29JTUK Department of Rehabilitation the Second People’s Hospital of ChengduChengdu610072China School of Acupuncture-Moxibustion and Tuina Chengdu University of Traditional Chinese MedicineChengdu610072China

Objective:To investigate whether the antihypertensive mechanism of electroacupuncture(EA)is associated with attenuating phenotype transformation of vascular smooth muscle cells(VSMCs)via phosphoinositide3-kinase(PI3K)/protein kinase B(Akt)and mitogen-activated protein kinase(mapk)signaling pathways.Methods:Eight Wistar-ktoyo(WKY)rats were set as normal blood pressure group(normal group).A total of 32 spontaneous hypertensive rats(SHRs)were randomly divided into 4 groups using random number tables:a model group,an EA group,an EA+PI3K antagonist group(EA+P group),and an EA+p38 mapk agonist+extracellular signal-regulated kinase(ERK)agonist group(EA+M group)(n=8/group).SHRs in EA group,EA+P group and EA+M group received EA treatment 5 sessions per week for continuous 4 weeks,while rats in the normal and model groups were bundled in same condition.The systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)of each rat was measured at 0 week and the 4th week.After 4-week intervention,thoracic aorta was collected for hematoxylin-eosin(HE)staining,immunohistochemistry[the contractile markersα-smooth muscle actin(α-SMA)and calponin and the synthetic marker osteopontin(OPN)]and Western blot[α-SMA,calponin,OPN,PI3K,phosphorylated-Akt(p-Akt),Akt,p-p42/44 ERK,total p42/44 ERK,p-p38 mapk and total p38 mapk].Results:EA significantly reduced SBP,DBP and MAP(Pmapk decreased in EA group(Pmapk agonist and ERK agonist.Conclusions:EA was an effective treatment for BP management.The antihypertensive effect of EA may be related with inhibition of phenotypic transformation

关键词： electroacupuncture hypertension vascular smooth muscle cells PI3K/Akt signaling pathway mapk signaling pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MiR-204-3p overexpression inhibits gastric carcinoma cell proliferation by inhibiting the mapk pathway and RIP1/MLK1 necroptosis pathway to promote apoptosis

引用

World Journal of Gastroenterology 2023年第29期29卷 4542-4556页

作者： Xia Li Joanna J Tibenda Yi Nan Shi-Cong Huang Na Ning Guo-Qing Chen Yu-Hua Du Ya-Ting Yang Fan-Di Meng Ling Yuan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Ningxia Chinese Medicine Reserch Center Yinchuan 750004Ningxia Hui Autonomous RegionChina Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Gastric carcinoma(GC)is the third most frequent cause of cancer-related death,highlighting the pressing need for novel clinical treatment options.In this regard,microRNAs(miRNAs)have emerged as a promising therapeutic strategy.Studies have shown that miRNAs can regulate related signaling pathways,acting as tumor suppressors or tumor promoters.AIM To explore the effect of miR-204-3p on GC cells.METHODS We measured the expression levels of miR-204-3p in GC cells using quantitative real-time polymerase chain reaction,followed by the delivery of miR-204-3p overexpression and miR-204-3p knockdown vectors into GC cells.CCK-8 was used to detect the effect of miR-204-3p on the proliferation of GC cells,and the colony formation ability of GC cells was detected by the clonal formation assay.The effects of miR-204-3p on GC cell cycle and apoptosis were detected by flow cytometry.The BABL/c nude mouse subcutaneous tumor model using MKN-45 cells was constructed to verify the effect of miR-204-3p on the tumorigenicity of GC cells.Furthermore,the study investigated the effects of miR-204-3p on various proteins related to the mapk signaling pathway,necroptosis signaling pathway and apoptosis signaling pathway on GC cells using Western blot techniques.RESULTS Firstly,we found that the expression of miR-204-3p in GC was low.When treated with the lentivirus overexpression vector,miR-204-3p expression significantly increased,but the lentivirus knockout vector had no significant effect on miR-204-3p.In vitro experiments confirmed that miR-204-3p overexpression inhibited GC cell viability,promoted cell apoptosis,blocked the cell cycle,and inhibited colony formation ability.In vivo animal experiments confirmed that miR-204-3p overexpression inhibited subcutaneous tumorigenesis ability in BABL/c nude mice.Simultaneously,our results verified that miR-204-3p overexpression can inhibit GC cell proliferation by inhibiting protein expression levels of KRAS and p-ERK1/2 in the mapk pathway,as well as inhibiting protein expression l

关键词： miR-204-3p Gastric carcinoma mapk signaling pathway Apoptosis Necroptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BcSDR1 is involved in regulation of glucose transport and cAMP and mapk signaling pathways in Botrytis cinerea

引用

Journal of Integrative Agriculture 2022年第9期21卷 2628-2640页

作者： SI He-long ZHANG Kang LI Bai YUAN Xue-mei ZANG Jin-ping CAO Hong-zhe XING Ji-hong DONG Jin-gao State Key Laboratory of North China Crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071000P.R.China

Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses. Our previous study showed that BcSDR1 positively regulates growth,development, and pathogenicity of B. cinerea. However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and mapk signaling pathways are not well understood. In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes. BcSDR1 mutant(BCt41) showed remarkably weak sensitivity to cAMP and mapk signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content. The key genes of cAMP and mapk signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated,whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated(P<0.05).BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference(RNAi) mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants. Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1expression.

关键词： Botrytis cinerea BcSDR1 glucose transmembrane transport cAMP signaling pathway mapk signaling pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immunoregulatory polysaccharides from Apocynum venetum L.flowers stimulate phagocytosis and cytokine expression via activating the NF-κB/mapk signaling pathways in RAW264.7 cells

引用

Food Science and Human Wellness 2022年第4期11卷 806-814页

作者： Honglin Wang Changyang Ma Dongxiao Sun-Waterhouse Jinmei Wang Geoffrey Ivan Neil Waterhouse Wenyi Kang National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Functional Food Engineering Technology Research Center Kaifeng 475004China School of Chemical Sciences University of AucklandAuckland 1142New Zealand Joint International Research Laboratory of Food&Medicine Resource Function Kaifeng 475004China

Two immunomodulatory polysaccharides(Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide(NO) and expression of inducible nitric oxide synthase(iNOS) m RNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ(100–800 μg/m L) and Vp3(400 μg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and m RNA expression of TNF-α and IL-6 in a concentrationdependent manner through affecting mitogen-activated protein kinase(mapk) activity and nuclear factor κB(NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the mapk signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and mapk signaling pathways without influencing the p38 mapk route.

关键词： Apocynum venetum L.flowers Immunomodulatory polysaccharide RAW264.7 cells NF-κB signaling pathway mapk signaling pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neuroprotective Effects of Raloxifene on Aβ_(25-35)-induced Damages in PC12 Cells via Mitogen-activated Protein Kinase signaling pathway

引用

Journal of Reproduction and Contraception 2012年第1期23卷 1-16页

作者： Xue DAI Jie WU Xue-ping SUN Li GAO Yu-gui CUI Jia-yin LIU Center of Clinical Reproductive Medicine Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011China Department of Obstetrics and Gynecology First Affiliated HospitalNanjing Medical UniversityNanjing 210029China

Objective To investigate the neuroprotective effects and the mechanism of this protection of raloxifene （RLX）, a selective estrogen receptor modulator.Methods MTT assay and flow cytometry with annexin V-FITC/PI staining were performed to evaluate the neuroprotective effects of RLX on Aβ25-35-induced toxicity. The potential mechanisms were studied by Western blotting in cultured rat pheochromocytoma cells （PC12 cells）.Results RLX（1 000 nmol/L）, in combination with Aβ25-35 （30 llmol/L）, increased the cell viability （P 〈0.001）, and reduced the number of apoptotic cells （P 〈0.05）. RLX attenuated Aβ25-35-induced loss of △ψm （P 〈0.01）. The changing of △ψm was similar to the variation of apoptosis. PD98059 （inhibitor of ERK1/2） inhibited the effects of RLX on cell viability and phosphorylation of cleaved caspase-9. No significant difference of cell viability or phosphorylation of cleaved caspase-9 had been found when PC12 cells were incubated with SB203580 （inhibitor of p38mapk） or SP600125 （inhibitor of JNK）. Afl25.35 induced a time-dependent phosphorylation of p38mapk and JNK. In PC12 cells treated solely with RLX, ERK1/2 was activated （P〈0.01）. In PC12 cells treated with Aβ25-35 and RLX, Aβ2545-induced phosphorylation of p38mapk and JNK were inhibited （P〈0.01 and P〈0.001, respectively）.Conclusion RLX inhibited Af125.35-induced cell apoptosis by activating the ERK1/2 pathway in PC12 cells. RLX also attenuated Aβ25-35-induced activation of p38mapk and JNK. The mitochondria pathway Was involved in this inhibitory effect.

关键词： raloxifene Aβ25-35 apoptosis mapk signaling pathway Alzheimer＇s disease mitogen-activated protein kinase （mapk）

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transcriptomic analysis of bisphenol AF on early growth and development of zebrafish(Danio rerio)larvae

引用

Environmental Science and Ecotechnology 2020年第4期4卷 1-11页

作者： Rongzhen Li Shuai Liu Wenhui Qiu Feng Yang Yi Zheng Ying Xiong Guanrong Li Chunmiao Zheng State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen518055China Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen518055China School of Environmental and Chemical Engineering Shanghai UniversityShanghai200444China Shenzhen Municipal Engineering Lab of Environmental IoT Technologies Southern University of Science and TechnologyShenzhen518055Guangdong ProvinceChina

Bisphenol AF(BPAF),an alternative to bisphenol A,is widely detected in aquatic environments.Owing to health concerns,the toxic effects of BPAF on organisms are drawing attention.The present study aims to evaluate the toxicity of BPAF,combining the results of omics techniques and experiment.Employing transcriptome sequencing(RNA-seq),we obtained 391,648,512,and 545 differentially expressed genes(DEGs)in 0.1,1,10,and 100 mg/L BPAF-exposed zebrafish larvae,respectively.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment revealed the early development,stimulus-response,and mapk signaling pathway were significantly affected by BPAF.In addition,five hub genes(fgf3,fgf4,map2k1,myca,and casp3b)were highlighted as the key genes in mapk signaling pathway using the protein-protein interaction network.Therefore,the RNA-seq results showed that early development and stimulus-response were the main processes affected by BPAF,which was consistent with our morphological and pathological results.The hatching rate of zebrafish embryos in 1 and 10 mg/L BPAF groups was significantly inhibited,and the oxidative stress indexes,including the level of total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and lipid peroxidation(LPO),were significantly increased by the 100 mg/L BPAF treatment.Moreover,the activity of alkaline phosphatase(AKP)was significantly decreased in all BPAF exposure groups.In conclusion,exposure to BPAF at environmental relevant concentrations affected the early development and immune system of zebrafish larvae by modulating mapk signaling pathway,and our results provide solid evidence for the future studies on the toxicity of bisphenols.

关键词： Bisphenol AF Zebrafish Early development Oxidative stress mapk signaling pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：