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Molecular cloning,identification,and chromosomal localization of two mads box genes in peach(Prunus persica)

Journal of genetics and Genomics 2008年第6期35卷 365-372页

作者： Lin Zhang Yong Xu Rongcai Ma College of Life Science Capital Normal University Beijing 100037 China Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China

mads box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two mads box genes, Ppmads1 and PpmadsIO, were cloned using degenerate primers and 5＇- and T-RACE based on the sequence database of P. persiea and P. duleis. The full length of Ppmads1 cDNA is 1,071 bp containing an open reading frame （ORF） of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpmadsIO cDNA is 937 bp containing an ORF of 633 bp and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that Ppmads1 and PpmadsIO were highly homologous to genes API and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that Ppmads1 belongs to the euAP1 clade of class A, and PpmadsIO is a member of GLO/PI clade of class B. RT-PCR analysis showed that Ppmads1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern ofAPl; Ppmads10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpmadsI was assigned onto the Binl：50 on the G1 linkage group between the markers MCO44 and TSA2, and PpmadsIO onto the Bin1：73 on the same linkage group between the markers Lap- 1 and FGA8. Our results provided the basis for further dissection of the two mads box gene function.

关键词： peach (Prunus persica) flower development mads box gene selective mapping

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Cloning and characterization of two cDNAs encoding rice mads box protein

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Progress in Natural Science:Materials International 2000年第5期 39-45页

作者：袁自强钱晓茵刘军刘建东钱旻杨金水 China Fudan University Institute of genetics Shanghai 200433

To elucidate the relationship between mads box gene and rice morphogenesis, RT-PCR with mads domain specific primers was performed to isolate mads box gene from young panicle of rice "Zhenshan 97B". Two cD-NAs, designated as nmadsl and nmadsS, displayed the structure of a typical plant mads box gene which consists of the mads domain, I region, K domain, and C-terminal region. Based on sequence homology, nmadsl is classified as a member of GLO subfamily, and nmadsS belongs to AGL2 subfamily. Hybridization analysis revealed that nmadsl and nmads3 were preferentially expressed in rice redifferentiated callus and young panicles but were not in rice seedling. An additional transcript of nmadsl was also found in young panicle of cytoplasmic male-sterile line Zhenshan 97A but was not in its maintenance line Zhanshan 97B.

关键词： mads box gene rice cDNA cloning morphogenesis.

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