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Stability of m13 phage in Organic Solvents

Journal of Biomaterials and Nanobiotechnology 2016年第2期7卷 72-77页

作者： Pouya moghimian Vesna Srot Benoit P. Pichon Sandra J. Facey Peter A. van Aken Stuttgart Center for Electron microscopy Max Planck Institute for Solid State Research Stuttgart Germany Institut de Physique et Chimie des matériaux de Strasbourg UMR 7504 CNRS—Université de Strasbourg Strasbourg France Institute of Technical Biochemistry University of Stuttgart Stuttgart Germany

The effect of different types of organic solvents on the structural integrity of m13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of m13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon exposure to polar organic solvents. We show that this finding can be beneficial for the macromolecular self-assembly and in broader aspects, to enhance the spatial arrangement of desired inorganic nanoparticles in the rapidly developing field of virotronics.

关键词： m13 phage Organic Solvent Structural Integrity Self-Assembly Electron microscopy

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Application of m13 phage Coat Proteins

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Agricultural Biotechnology 2013年第1期2卷 45-47页

作者： Zhi DING Ling ZHAO Xuemei mA Beijing University of Technology

m13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of m13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.

关键词： m13 phage coat protein phage display technology

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Bioprocess-inspired preparation of silica with varied morphologies and potential in lithium storage

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Journal of materials Science & Technology 2021年第13期72卷 61-68页

作者： Fuqiang Wan Wenxuan Wang Zhaoyong Zou Hao Xie Hang Ping Zhengyi Fu State Key Laboratory of Advanced Technology for materials Synthesis and Processing Wuhan University of TechnologyWuhan430070China School of Chemistry Chemical Engineeringand Life ScienceWuhan University of TechnologyWuhan430070China

As one of the most delicate bioprocesses in nature,biosilicification is closely related to biosilica with various morphologies,and has provided abundant inspiration to materials *** the present study,to explore the biosilica formation process and fabricate silica with an exquisite microstructure for lithiumion battery(LIB)electrodes,a bacterial phage(m13)is used as a biotemplate to synthesize silica with diverse morphologies:cylinders,hexagonal prisms,assemblies of smaller cylinders and nanowires.A facile ethanol bath method is conducted to coat the nanowires with nitrogen-containing carbon and carbon-coated SiO_(2) nanowires with mesochannels(C@msSiO_(2) NWs)are first used as anode materials for *** to the uniform carbon coating and parallel mesochannel structure,the electronic conductivity and capacity to accommodate volume variations were significantly *** the electrochemical perfo rmance test,the composites calcined at 750℃(C@msSiO_(2) NWs-750)show an impressive capacity of 653 mA h g^(-1) at a current density of 500 mA g^(-1) and stability(1000 cycles).In view of the electrochemical test outcomes,the prepa ration of a sophisticated structure with an outstanding potential is easily achieved via a biomimetic strategy.

关键词： Biomimetic preparation Biosilicification m13 phage Silica nanowires mesochannel

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