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Eco-Friendly pH Indicator Based on Natural Anthocyanins from lycium ruthenicum

Journal of Donghua University(English Edition) 2022年第2期39卷 110-114页

作者： ZHANG Hongjie LIAN Yasi YU Jiayan LU Run ZHANG Jie QIU Yiping FENG Lili College of Textiles and Apparel Quanzhou Normal UniversityQuanzhou 362000China Shishi Institute for Ecological&Smart Textile Quanzhou Normal UniversityQuanzhou 362000China College of Textiles Donghua UniversityShanghai 201620China

An eco-friendly and visual pH indicator was developed based on cotton fabrics dyed with natural lycium ruthenicum extract(LRE),whose color changed from red to purple,blue and green at pH of *** methods for dyeing cotton fabrics with LRE were attempted,namely pre-mordanting,simultaneous mordanting,and post-mordanting methods with different dyeing temperatures,dyeing times and dyebath pH *** cotton fabrics exhibited the highest K/S value when they were dyed under a dyebath of pH 6 at 20℃for 90 min in the case of the simultaneous mordanting ***,the dyed cotton fabrics also showed reversible pH-dependent color *** developed flexible pH indicator based on renewable natural materials is suitable for multiple applications in environmental monitoring and smart textiles.

关键词： lycium ruthenicum anthocyanins pH indicator environmental monitoring smart textiles

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Physiological characterization,transcriptomic profiling,and microsatellite marker mining of lycium ruthenicum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第11期18卷 1002-1021页

作者： Jin-huan CHEN Dong-zhi ZHANG Chong ZHANG Mei-long XU Wei-lun YIN College of Biological Sciences and Technology Beijing Forestry UniversityBeijing 100083China National Engineering Laboratory for Tree Breeding Beijing Forestry UniversityBeijing 100083China State Key Laboratory of Seedling Bioengineering Yinchuan 750004China

lycium ruthenicum is a perennial shrub species that has attracted considerable interest in recent years owing to its nutritional value and ability to thrive in a harsh environment. However, only extremely limited transcriptomic and genomic data related to this species can be found in public databases, thereby limiting breeding research and molecular function analysis. In this study, we characterized the physiological and biochemical responses to saline- alkaline mixed stress by measuring photochemical efficiency, chlorophyll content, and protective enzyme activity. We performed global transcriptomic profiling analysis using the IIlumina platform. After optimizing the assembly, a total of 68063 unique transcript sequences with an average length of 877 bp were obtained. Among these sequences, 4096 unigenes were upregulated and 4381 unigenes were down-regulated after saline-alkaline mixed treatment. The most abundant transcripts and over-represented items were assigned to gene ontology （GO） terms or Kyoto Encyclopedia of Genes and the Genomes （KEGG） categories for overall unigenes, and differentially expressed unigenes were analyzed in detail. Based on this set of RNA-sequencing data, a total of 9216 perfect potential simple sequence re- peats （SSRs） were identified within 7940 unigenes with a frequency of 1/6.48 kb. A total of 77 primer pairs were synthesized and examined in wet-laboratory experiments, of which 68 loci （88.3%） were successfully amplified with specific products. Eleven pairs of polymorphic primers were verified in 225 individuals from nine populations. The inbreeding coefficient and the polymorphism information content value ranged from 0.011 to 0.179 and from 0.1112 to 0.6750, respectively. The observed and expected heterozygosities ranged from 0.064 to 0.840 and from 0.115 to 0.726, respectively. Nine populations were clustered into three groups based on a genetic diversity study using these novel markers. Our data will be useful for functional genom

关键词： De novo Genetic diversity lycium ruthenicum Molecular marker Saline-alkaline mixed stress

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