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Mass rearing of lucilia sericata Meigen (Diptera:Calliphoridae)

Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 54-56页

作者： Firoozfar F Moosa-Kazemi H Baniardalani M Abolhassani M Khoobdel M Rafinejd J Department of Medical Entomology School of Public HealthTehran University of Medical Sciences Mehdi Khoobdel Health Research CenterBaqiyatallah University of Medical Sciences

Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,*** samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic ***:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj *** the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,***:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.

关键词： Rearing lucilia sericata Mass Rearing Sheep blow fly Maggot therapy Systematic keys Insectary condition

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Antibacterial properties of lucifensin in lucilia sericata maggots after septic injury

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Asian Pacific Journal of Tropical Biomedicine 2014年第5期4卷 358-361页

作者： Ivana Valachova Emanuel Prochazka Jana Bohova Petr Novak Peter Takac Juraj Majtan Institute of Zoology Slovak Academy of Sciences Institute of Microbiology Academy of Sciences of the Czech Republic Scientica s.r.o.

Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic ***:In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary *** is assumed that lucifensin is secreted solely from this tissue into the haemoiymph(similar to other insect defensins) and not into secreted/excreted *** used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemoiymph extracted from larvae after septic ***:After septic injury,production of lucifensin in the haemoiymph is *** led to higher antibacterial activity of such haemoiymph in comparison to non-stimulated ***:These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy,lucifensin is simultaneously important as a part of the systematic immune response.

关键词： lucilia sericata Wound bacteria Defensin Lucifensin Immune-challenge

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Cutaneous Myiasis: Is <i>lucilia cuprina</i>Safe and Acceptable for Maggot Debridement Therapy?

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第2期2卷 79-82页

作者： Howard Joesphia Kingu Simon Kamande Kuria Martin Herrer Villet Jane Nthekeleng Mkhize Adupa Dhaffala John Michael Iisa Department of Biological Sciences Walter Sisulu University Mthatha South Africa Department of Entomology and Zoology Rhodes University Grahamstown South Africa Department of Surgery Walter Sisulu University Mthatha South Africa

Preservation of viable tissue is important in wound management. It is achieved by small, incremental removal of devitalised, necrotic and infected tissues. Maggot debridement therapy (MDT) is used in septic necrotic wounds that fail to respond to conventional modalities. MDT has relied on lucilia cuprina, which consumes only necrotic tissues, as opposed to lucilia cuprina, which devours both flesh and necrotic tissues. Recent findings have shown that L. cuprina consumes mainly necrotic and very small amounts of viable tissues and may be used in MDT where L. sericata is very rare or absent. Here we describe wound healing in a patient from rural South Africa with cutaneous myiasis. Our findings agree with workers who indicated that L. cuprina could be used in MDT.

关键词： Maggots Blow Flies lucilia cuprina Mthatha

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