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Oncology and Translational Medicine 2016年第2期2卷 84-89页

作者： Xiaoran Liu Donghai Liang Tao Jiang Qing Dong Hongsheng Yu Qingdao University

Objective Ovarian cancer is one of the leading causes of mortality in patients with malignant gynecological tumors. After surgical intervention for ovarian cancer, cisplatin（DDP）-based chemotherapy is the first-line treatment. However, a major challenge to treating ovarian cancer is the development of chemoresistance. Thus, the first aim of this study was to determine whether low-dose radiation could enhance the susceptibility of resistant ovarian cancer cells to DDP. The second aim was to provide new strategies for treating DDP-resistant ovarian cancer by examining its *** A cell counting kit-8（CCK8） assay was performed to measure cell proliferation. Flow cytometry was utilized to quantify the apoptosis of DDP-resistant ovarian cancer cells（SKOV3/DDP） using Annexin V and propidium iodide staining. Real-time quantitative（q PCR） was used to analyze the messenger RNA（m RNA） expression levels of excision repair cross complementing-group 1（ERCC1） and B-cell lymphoma 2（Bcl-2） in SKOV3/*** The IC50values of the control, conventional-dose, and low-dose groups were 9.367 ± 0.16, 9.289 ± 0.16, and 3.847 ± 0.15, respectively（P dose group）. Compared with the control and conventional-dose groups, low-dose radiation resulted in significantly more apoptosis, as detected by flow cytometry（P low-dose group was significantly lower than that in the control group and conventional-dose group（P low-dose radiation enhanced the sensitivity of resistant ovarian cancer cells to DDP, possibly by decreasing the DNA repair capacity of tumor cells and promoting apoptosis.

关键词： low-dose radiation ovarian cancer resistance SKOV3/DDP excision repair cross complementing-group 1(ERCC1) B-cell lymphoma 2(Bcl-2)

Mitochondrial Modulation of Apoptosis Induced by low-dose radiation in Mouse Testicular Cells

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Biomedical and Environmental Sciences 2013年第10期26卷 820-830页

作者： FANG Fang GONG Ping Sheng ZHAO Hong Guang BI Yu Jing ZHAO Gang GONG Shou Liang WANG Zhi Cheng Key Laboratory of Radiobiology Ministry of HealthSchool of Public HealthJilin University Key Laboratory for Molecular Enzymology and Engineering Ministry of Education Nuclear Medicine First Hospital of Jilin University State Key Laboratory of Pathogen and Biosecurity Academy of Military Medical Sciences

Objective To investigate whether apoptosis induced by low-dose radiation （LDR） is regulated by mitochondrial pathways in testicular cells. Methods Male mice were exposed to whole-body LDR, and changes in mitochondrial function and in expression of apoptotic factors were analyzed in the testicular cells as follows. Total nitric-oxide synthase （T-NOS） and Na＋/K＋ ATPase activities were biochemically assayed. Reactive oxygen species （ROS） and mitochondrial membrane potential （Adjm） were determined by flow cytometry using fluorescent probes. Levels of mRNAs encoding cytochrome c （Cyt c） and apoptosis-inducing factor （AIF） were quantified by real-time reverse-transcription PCR （RT-PCR）. Expression of Cyt c, AIF, caspase-9, and caspase-3 at the protein level was assessed by western blotting and immunohistochemistry. Results LDR induced an increase in T-NOS activity and ROS levels, and a decrease in Na＋/K~ ATPase activity and mitochondrial A^m, in the testicular cells. The intensity of these effects increased with time after irradiation and with dose. The cells showed remarkable swelling and vacuolization of mitochondria, and displayed a time- and dose-dependent increase in the expression of Cyt c, AIF, procaspase-9, and procaspase-3. Activation of the two procaspases was confirmed by detection of the cleaved caspases. The changes in expression of the four apoptotic factors were mostly limited to spermatogonia and spermatocytes. Conclusion LDR can induce testicular cell apoptosis through mitochondrial signaling pathways

关键词： Mitochondria low-dose radiation Testis Apoptosis Cytochrome c

Alzheimer's disease and low-dose radiation therapy:A new hope

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radiation Medicine and Protection 2024年第1期5卷 1-6页

作者： Neal E.Dunlap Robert P.Friedland Lu Cai Department of radiation Oncology University of Louisville School of MedicineLouisvilleKY40202USA Department of Neurology The University of Louisville School of MedicineLouisvilleKY40202USA Pediatric Research Institute Departments of PediatricsPharmacology and ToxicologyUniversity of Louisville School of MedicineLouisvilleKY40202USA

The concept of low-dose radiation(LDR)-induced hormetic responses was initially observed approximately 70 years ago and systematically reviewed along with the discovery of LDR-induced adaptive responses in a cytogenetic in vitro study in *** the end of the 1990s,discussions regarding the potential applications of LDRinduced hormesis and adaptive responses for preventing or treating chronic diseases,such as Alzheimer’s disease(AD)had taken *** 2016,reports on radiotherapy for the subjects with AD and for genetic AD model mice were ***,several preclinical studies with animal models of AD and clinical studies in AD subjects were conducted.A significant milestone was achieved with the online availability of a new Systematic Review based on qualified publications from these preclinical and clinical *** mini-review provides a concise historical introduction to LDR-induced hormesis and adaptive responses with discussion of AD radiotherapy with either LDR or relatively high dose *** of this Systematic Review cover promising outcomes,challenges,and new questions,followed by discussion of potential mechanisms.

关键词： Alzheimer’s disease low-dose radiation radiation adaptive response radiation hormesis

low dose radiation induced adaptive response of apoptosis in mouse spleen cells

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The Chinese-German Journal of Clinical Oncology 2010年第4期9卷 235-238页

作者： Hongsheng Yu Ning Liu Bo Ju Department of Oncology The Affiliated Hospital of Medical College Qingdao University Ojngdao 266003 China

Objective:The aim of the study was to investigate the adaptive response of low-dose radiation-induced apoptosis in mouse spleen cells and its related proteins control mechanism. Methods: Kunming male mice were randomly divided into sham-irradiation group, attacking dose irradiation group (D2) and low-dose radiation + attacking dose irradiation group (D1+D2). After a week the D1+D2 group was given low-dose radiation (doses were 25,50,75 and 100 mGy, dose rate of 12.5 mGy/min), after 6 hours the D2 group and the D1+D2 group were given attacking dose of radiation (dose of 1.5 Gy, dose rate of 287 mGy/min). After 18 hours all mice were killed. The spleen cell apoptosis and Caspase-3 expression's changes were detected by flow cytometry (FCM) analysis, and the changes of apoptosis-related proteins Bcl-2 and Bax were detected by immunohistochemical staining semi-quantitative method. Results: The rate of apoptosis, the fluorescence intensity of Caspase-3, and the semi-quantitative results of apoptosis-related proteins Bcl-2 and Bax in every group were significantly different. Of all groups, the apoptosis rate and Caspase-3 fluorescence intensity of D2 group were the highest. Compared with the sham-irradiated group, Bcl-2 protein level of D2 group decreased significantly, and Bax protein level of D2 group increased significantly. But compared with D2 group, the apoptosis rates, the fluorescence intensities of Caspase-3 expression and the Bax protein levels in D1+D2 group were lower in different doses, and the Bcl-2 protein levels in D1+D2 group were higher in different doses. Conclusion: 25-75 mGy low-dose radiation can induce the adaptive response of mouse spleen cells' apoptosis, the expression of Caspase-3, and the apoptosis-related protein Bcl-2 expression increased, Bax reduced, and it leads to the apoptosis of the spleen cells decreased.

关键词： low-dose radiation spleen apoptosis apoptosis-related proteins

Local low-dose X-ray radiation promotes homing of mesenchymal stem cells to the injured mouse spinal cord

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Neural Regeneration Research 2010年第5期5卷 331-336页

作者： Panjun Wang Xiaohui Zhang Leixi Xue Yu Sun Songguang Ju Liesong Chen Jiasheng Hu Hong Zhang Xueguang Zhang Jinxiang Fu Department of Hematology Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu Province China Department of Hematology First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Institute of Biotechnology School of Life Sciences Soochow University Suzhou 215007 Jiangsu Province China Department of Radiotherapy Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu Province China

BACKGROUND： Bone marrow-derived mesenchymal stem cells （BMSCs） are a potentially useful source for cell replacement therapy following spinal cord injury. However, the homing characteristics of BMSCs in vivo remain unclear. low-dose radiation has been shown to promote homing of BMSCs to exposed sites. OBJECTIVE： To investigate the effects of low-dose local radiation to non-injured areas on the ability of human BMSCs to home to the injured mouse spinal cord, as well as recovery of spinal cord injury. DESIGN, TIME AND SE＇I-FING： A randomized, controlled, animal experiment was performed at the Central Laboratory, Second Affiliated Hospital of Soochow University between October 2007 and October 2008. MATERIALS： BMSCs were isolated from four adult, human donors. METHODS： Fifty adult, female, Balb/c mice were subjected to adjusted weight-drop impact resulting in complete paraplegia. Three days later, mice were randomly assigned to a radiation ＋ transplantation group （n = 23） and a transplantation group （n = 20）. In total, 2 x 106 carboxyfluorescein diacetate succinimidyl ester-labeled BMSCs were injected into each mouse via the caudal vein. Mice in the radiation ＋ transplantation group received 2.5 Gy local X-ray irradiation 2 hours before BMSCs injection. MAIN OUTCOME MEASURES： The homing of BMSCs to injured cord and irradiated skin after transplantation was observed by fluorescence microscope; the structure recovery of injured cord was assessed by magnetic resonance imaging. RESULTS： Compared with the transplantation group, at 24 hours after transplantation, the number of BMSCs was significantly increased in the injured area and the exposed site （P 〈 0.05）, and inflammation and edema were significantly alleviated in the injured cord in the radiation ＋ transplantation group. CONCLUSION： Local low-dose radiation has the potential to promote homing of BMSCs and recovery of spinal cord injury, although the radiated region was not injured area.

关键词： low-dose radiation bone marrow-derived mesenchymal stem cells spinal cord injury cell therapy neural regeneration

Effect of low-dose X-ray radiation on adaptive response in gastric cancer cell

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The Chinese-German Journal of Clinical Oncology 2013年第4期12卷 171-174页

作者： Shukai Wang Gang Jiang Hongsheng Yu Xiangping Liu Chang Xu Department of Oncology The Affiliated Hospital of Medical College Qingdao University

Objective： We aimed to study the effect and mechanism of low-dose radiation （LDR） on adaptive response of gastric cancer cell. Methods： SGC7901 cells were cultured in vitro, and divided into 4 groups： control group （DO group）, low-dose radiation group （D1 group, 75 mGy）, high-dose radiation group （D2 group, 2 Gy）, low-dose plus high-dose radiation group （D1 ＋ D2 group, 75 mGy ＋ 2 Gy, the interval of low and high-close radiation being 8 h）. Cell inhibition rate was detected by cytometry and CCK8 method; the proportion of cell cycle at different times after irradiation was determined by using a flow cytometry. The ATM mRNA levels were detected by using quantitative real-time reverse transcription polymerase chain reaction （RT-PCR）. Results： There was no significant different between groups DO and D1, groups D2 and D1 ＋ D2 cell inhibition rate （P 〉 0.05）. There was a significant increase G2/M arrest in groups D2 and D1 ＋ D2 than groups DO and D1 after 6 h of radiation and did not recover at 48 h （P 〈 0.05）. The ATM mRNA expression of group D2 and D1 ＋ D2 increased highly than that of group DO and D1 （P 〈 0.05）. However, differences between group D2 and D1 ＋ D2, group DO and D1 were not statistical significant （P 〉 0.05）. Conclusion： LDR cannot induce adaptive response in SGC7901 cells in vitro, which may be associated the regulation of cell cycle, and its ATM mRNA expression cannot be affected by 75 mGy X-ray radiation.

关键词： low-dose radiation SGC7901 cells adaptive response cell cycle