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Comprehensive and deep profiling of the plasma proteome with protein corona on zeolite NaY

Journal of Pharmaceutical Analysis 2023年第5期13卷 503-513页

作者： Congcong Ma Yanwei Li Jie Li Lei Song Liangyu Chen Na Zhao Xueping Li Ning Chen Lixia Long Jin Zhao Xin Hou Li Ren Xubo Yuan Tianjin Key Laboratory of Composite and Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin300350China Department of Integrative Oncology Tianjin Medical University Cancer Institute and Hospital and Key Laboratory of Cancer Prevention and TherapyTianjin300060China Department of Proteomics Tianjin Key Laboratory of Clinical Multi-omicsTianjin300308China Department of Clinical Laboratory Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin's Clinical Research Center for CancerTianjin300060China

Proteomic characterization of plasma is critical for the development of novel pharmacodynamic ***,the vast dynamic range renders the profiling of proteomes extremely ***,we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and deep profiling of the plasma proteome using the plasma protein corona formed on zeolite ***,zeolite NaY and plasma were co-incubated to form plasma protein corona on zeolite NaY(NaY-PPC),followed by conventional protein identification using liquid chromatography-tandem mass *** was able to significantly enhance the detection of low-abundance plasma proteins,minimizing the“masking”effect caused by high-abundance *** relative abundance of middleand low-abundance proteins increased substantially from 2.54%to 54.41%,and the top 20 highabundance proteins decreased from 83.63%to 25.77%.Notably,our method can quantify approximately 4000 plasma proteins with sensitivity up to pg/mL,compared to only about 600 proteins identified from untreated plasma samples.A pilot study based on plasma samples from 30 lung adenocarcinoma patients and 15 healthy subjects demonstrated that our method could successfully distinguish between healthy and disease *** summary,this work provides an advantageous tool for the exploration of plasma proteomics and its translational applications.

关键词： NaY Plasma proteomics Protein corona low-abundance proteins

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Genetically Encoded Multifunctional Protein Photocrosslinker

Genetically Encoded Multifunctional Protein Photocrosslinker

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第十一届全国化学生物学学术会议

作者： Xiao Xie Dan He Peng Chen The College of Chemistry and Molecular Engineering Peking University

Protein-protein interactions play vital roles in various biological processes. Compared with other methods, genetically encoded photocrosslinking could convert non-covalent interactions to covalent bond and has emerged as a suitable strategy for in situ and in vivo trapping of dynamic or weak interactions with high-spatial resolution. [1] Nevertheless, developed photocrosslinkers always result in high backgrounds, low signal-to-noise ratio and difficulties in detection of low-abundance proteins. Therefore, we developed a quantitative and comparative proteomics strategy that relies on a genetically encoded trifunctional photocrosslinker（DiZASeC） bearing a Se-based releasable linker and an alkyne-diazirine minimalist moiety, which allowed us to perform the isoCAPP strategy for identifying the proteolytic substrates of an essential *** PQC factor DegP under heat shock conditions or acid stress. [2]

关键词： Genetically encoded Multifunctional low-abundance proteins Quantitative proteomics

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