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Three new secoiridoid glycosides from the flower buds of Lonicera japonica

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Chinese Journal of Natural Medicines 2020年第1期18卷 70-74页

作者： YANG Ran HAO Hao LI Jia XUAN Jing XIA Ming-Feng ZHANG Yong-Qing Key laboratory of Natural Pharmaceutical Chemistry Shandong University of Traditional Chinese MedicineJinan 250200China Key laboratory of Pharmacology Shandong University of Traditional Chinese MedicineJinan 250200China

Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the structures were identified by spectroscopic analyses.Lonijapoglycol A(1)expressed significant anti-inflammatory activity to inhibit the release ofβ-glucuronidase induced by platelet-activating factor in rat polymorphonuclear leukocytes with an IC50 value of 3.76μmol·L^-1.

关键词： Lonicera japonica Secoiridoid glycosides Spectroscopic analysis Anti-inflammatory activity

Comparison of anti-bacterial activity of three types of di-O-caffeoylquinic acids in Lonicera japonica flowers based on microcalorimetry

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Chinese Journal of Natural Medicines 2014年第2期12卷 108-113页

作者： HAN Jin LV Qing-Yuan JIN Shi-Ying ZHANG Tian-Tian JIN Shi-Xiao LI Xian-Yi YUAN Hai-Long 302 Military Hospital of China Institute for Drug and Instrument Control Health Department GLD of PLA

The anti-bacterial activities of three types of di-O-caffeoylquinic acids(diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine(TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid(3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid(3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid(4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power–time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs(IC50) were obtained by quantitative analysis. Based on the quantity–activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.

关键词： di-O-caffeoylquinic acids Lonicera japonica Bacillus shigae Antibacterial activity Microcalorimetry

Study on Tissue Culture of Japanese Honeysuckle ( Lonicera japonica )

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Agricultural Biotechnology 2023年第5期12卷 9-13,18页

作者： Fangjun LIAO Xiaoru CHEN Huizhou Engineering Vocational College Huizhou 516000China

[Objectives]This study was conducted to increase the reproduction coefficient of Japanese honeysuckle(Lonicera japonica)to keep the character of optimal benign.[Methods]The young leaves of medicinal Japanese honeysuckle were selected as explants,and MS was used as the basic culture medium.Suitable culture concentrations and conditions were screened through different concentration gradients of growth regulators and cytokinin.[Results]As the concentration of 6-BA in the culture medium increased,the browning rate increased,and the browning phenomenon occurred earlier.On the contrary,a lower concentration of 6-BA was suitable for the differentiation and growth of young leaves,and the browning response was slow.However,if the cultivation time was too long and the materials were not transferred in a timely manner,browning would also occur.The optimal combination of levels was obtained through a 3×3 orthogonal experiment(three parallel groups for each of 6-BA and NAA).The culture conditions included a constant temperature of 26℃and light intensity of 1200 lx.The optimal medium for inducing callus proliferation was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;and the optimal medium for inducing bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.[Conclusions]This study provides a theoretical basis for accelerating the development of the honeysuckle industry.

关键词： Lonicera japonica Explants Growth regulator Cytokinin

Lonicera japonica-induced inhibition of interleukin-1 beta thermogenesis and E-type prostaglandin receptor-3 expression in the preoptic area of rabbits

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Neural Regeneration Research 2008年第2期3卷 204-207页

作者： Jun Dong Rongbo Tu Rui Pan Xinhua Xie Department of Pathophysiology Medical College of Jinan University Guangzhou 510632 Guangdong Province China Department of Orthopaedics The First Affiliated Hospital of Jinan University Guangzhou 510632 Guangdong Province China

BACKGROUND： It has been shown that interleukin-1β（IL-1β） can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2, which binds with receptors of the thermo-sensitive hypothalamic neurons. Lonicera japonica is one of the medicinal plants used widely in Asia for its antipyretic properties. However, these mechanisms have not yet been intensively studied. OBJECTIVE： To investigate the antipyretic effect and mechanisms of Lonicera japonica on IL-1β- induced febrile New Zealand rabbits by observing expression changes of E-type prostaglandin receptor-3 （EP3） mRNA in the preoptic anterior hypothalamus （POAH）. DESIGN： A randomized controlled study. SETTING： Electrophysiological Laboratory at the Department of Pathophysiology, Medical College of Jinan University; Department of Orthopaedics, First Hospital Affiliated to Medical College of Jinan University. MATERIALS： The experiment was performed from April to December 2005, using a total of 32 New Zealand white rabbits of both sexes, weighing 1.5 2.0 kg. All the animal experiments were performed according to the internationally accepted ethical guidelines. Lonicera japonica injection was purchased from Huanghe pharmaceutical factory of Xi＇an, China. IL-1βwas purchased from Sigma, USA. METHODS： A total of 32 rabbits were divided randomly into four groups： ① Normal saline （NS） control group;② lonicerajaponica treatment group; ③ IL-1βtreatment group; and ④lonicerajaponica plus IL-1βtreatment group. In the first 3 groups, the rabbits were given separate intravenous （i.v.） injections of l mL NS, l mL Lonicera japonica, and 100 ng IL-l β （dissolved in 0.9% NS without pyrogen）. In the lonicerajaponica plus IL-1βgroup each rabbit was given i.v. injections of l mL NS and, 30 minutes later, 100 ng IL-1 β. MAIN OUTCOME MEASURES： Colonic temperature of each rabbit was measured at 0, 10, 20, 30, 40, 50, 60, and 70 minutes after injection and the maximum temperature rise （ A T） and the temperature response ind

关键词： Lonicera japonica interleukin-1β antipyretic preoptic anterior hypothalamus E-type prostaglandin receptor in situ hybridization

Complete Chloroplast Genome Sequence of Medicinal Plant Lonicera japonica Thunb

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Complete Chloroplast Genome Sequence of Medicinal Plant Loni...

Two Triterpenoid Saponins from Lonicera japonica

第十四届全国中药和天然药物学术研讨会

作者： Liu He Jun Qian Xiaolan Xu Zhiying Sun Wei Xiao Xiwen Li Shilin Chen

Lonicera japonica is cultivated as ornamental and medicinal plant in the world.Using 454 pyrosequencing the complete chloroplast genome sequence of L.japonica is determined.The length of circular double-stranded DNA is 155,078 bp,contains a pair of inverted repeat regions（IRa,IRb） of 23,774 bp each and the large（LSC,88,858 bp） and small（SSC,18672bp） single-copy regions.The genome contains 83 proteins,31 tRNAs and 4 rRNAs genes.A total of 11 forward,9 palindromic and 13 tandem repeats were detected in the L.japonica cp genome.The genome structure,gene order,GC content and codon usage are similar to the typical angiosperm cp genomes.Most simple sequence repeats（SSR） are AT-rich and are less abundant in coding regions than in non-coding regions.Both SSRs and GC content were unevenly distributed in the entire cp genome.All preferred synonymous codons were found to use A/T ending codons.Phylogenetic analysis based on 71 protein-coding genes supported L.japonica clustered together with Araliaceae.This study provides valuable information for phylogenetic and cp genetic engineering studies of the medicinal plant.

关键词： Lonicera japonica Chloroplast genome 454

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Chinese Chemical Letters 2000年第4期11卷 337-340页

作者： Rong Wei TENG De Zu WANG Chang Xiang CHEN (Kunming Institute of Botany. Chinese Academic of Sciences. Kunming 650204) Kunming Institute of Botany Chinese Academic of Sciences

A new triterpenoid saponin, together with a known saponin. was isolated from the flowers of Lonicera japonica Thunb. Using chemical and spectroscopic methods, mainly 2D NMR technique, their structures were deduced to be 3-beta-D-glucopyranosyl-(1 --> 4)-beta-D-glucopyranosyl-(1 --> 3)-alpha-L-rhamnospyranosyl-(1 --> 2)-alpha-L-arabinopyranosyl hederagenin 28-O-beta-D-glucopyranosyl-(l --> 6)-beta-D-glucopyranoside and 3-alpha-L-rhamnospyranosyl-(1 --> 2)-alpha-L-arabinopyranosyl hederagenin 28-O-alpha-L-rhamnospyranosyl- (1 --> 2)-[beta-D-xylcopyranosyl-( 1 --> 6)]-beta-D-glucopyranoside, respectively.

关键词： triterpnoid saponin Lonicera japonica 2D NMR

Epigenetic changes in the regulation of carotenoid metabolism during honeysuckle flower development

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Horticultural Plant Journal 2023年第3期9卷 577-588页

作者： Hang Yu Na Cui Kun Guo Wen Xu Haifeng Wang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Key Lab of Sugarcane BiologyCollege of AgricultureGuangxi UniversityNanningGuangxi 530005China Institute of Medicinal Plant Development Chinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100193China Innovation and Transformation Center of Science and Technology College of PharmacyFujian University of Traditional Chinese MedicineFuzhouFujian 350122China

Flower development is one of the most vital pathways in plant development, during which the epigenetic regulation of gene expression is essential. DNA methylation, the most conserved epigenetic modification, participates in gene expression regulation and transposable element silencing. Honeysuckle(Lonicera japonica) is an important medicinal plant renowned for its colorful and fragrant flowers. Honeysuckle flowers change color from white to gold as a result of carotenoid accumulation during development. However, the role of DNA methylation in flower color changes is not well understood in L. japonica. Here, we performed whole-genome bisulfite sequencing and transcriptome sequencing during flowering development in honeysuckle. The results showed that a decrease in the levels of genome-wide average DNA methylation during flower development and changes in DNA methylation were associated with the expression of demethylase genes. Moreover, many genes involved in carotenoid biosynthesis and degradation, such as Lj PSY1, LjPDS1, LjLCYE, and LjCCD4, have altered expression levels because of hypomethylation, indicating that DNA methylation plays an important role in flower color changes in honeysuckle. Taken together, our data provide epigenetic insights into flower development and color change in honeysuckles.

关键词： Lonicera japonica DNA methylation Epigenetic regulation Gene expression Carotenoid metabolism

Metrics for macroscale invasion and dispersal patterns

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Journal of Plant Ecology 2018年第1期11卷 64-72页

作者： Teresa L.Clark Basil V.IannoneⅢ Songlin Fei Department of Forestry and Natural Resources Purdue University715 W.State StreetWest LafayetteIN 47907USA School of Forest Resources and Conservation University of Florida1745 McCarty DriveGainesvilleFL 32611USA

Aim The ability to quantitatively measure the continuum of macroscale patterns of species invasion is a first step toward deeper understand-ing of their causal factors.We took advantage of two centuries worth of herbarium data,to evaluate a set of metrics to measure macroscale patterns,allowing cross-species comparisons of inva-sive expansion across large geographic areas.Methods We used herbarium specimens to reconstruct county-level invasion histories for two non-native plants(Alliaria petiolata and Lonicera japonica),with distinct spatiotemporal distribution patterns over the past two centuries.Using county centroids from species’ini-tial occurrences,we quantified point pattern metrics from multiple disciplines(e.***.urban crime analysis,landscape ecology etc.)that are historically used at smaller spatial scales,to evaluate their abil-ity to detect macroscale spatial diffusion and amount of directional expansion.Metrics were further assessed for their ease of use,data requirements,independence from other metrics and intuitiveness of interpretation.Important Findings We identified four suitable metrics for distinguishing differences in spatial patterns:(i)standard distance,(ii)number of patches,(iii)Euclidean nearest neighbor summary class statistic coefficient of variation and(iv)mean center that when applied to county-level presence data allowed us to determine the directions by which distributions expanded and if distributions increased via outward expansion,infilling and/or jump dispersal events.These metrics when compared during the same invasion phase are capable of quantifying macroscale variability among species in their distri-butional and dispersal patterns.Being able to quantify differences among species in these patterns is important in understanding the drivers of species dispersal patterns.These metrics therefore repre-sent a simple yet thorough toolset for achieving this goal.

关键词： Alliaria petiolata diffusion herbarium jump dispersal Lonicera japonica plant invasions

Bioactivity Determination and Control Effect Evaluation of Three Plant Secondary Metabolites against Semiaphis heraclei

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Plant Diseases and Pests 2021年第4期12卷 1-4,8页

作者： Panpan Sun Luyao Huang Min Li Longtai Ju Yumeng Wu Jia Li College of Pharmaceutical Science Shandong University of Traditional Chinese MedicineJinan 250355China

[Objective] The paper was to clarify the bioactivity of trans-2-hexenal,oregano oil and thyme oil against Semiaphis heraclei and the synergistic effect of orange peel essential oil and organosilicon on the aphidicidal activity of the three agents.[Method] With S.heraclei,the dominant species of aphids in Lonicera japonica thunb.,as the test insect,the bioactivity and control effect were evaluated by the method of leaf dipping and foliar spraying.[Result] The indoor toxicity of thyme oil was the highest,with the LC_(50) of 1.793 mg/L.Adding additives significantly increased the toxicity of the agents to S.heraclei.Among them,trans-2-hexenal+OSi,oregano oil+OSi and thyme oil+OSi had obvious synergistic effects,with the toxicity coefficients of 1.80,1.48 and 1.45,respectively.Field test showed that the corrected control effect of thyme oil was the highest after con-ventional spraying,and the toxicities of the three agents were higher than that of the control group at 7 d post spraying.Under the condition of 20%reduction of three agents and adding organosilicon,the control effect was increased by about 10% at 1 d post spraying,which was higher than that of the control group at 4 d post spraying,and reached over 98% at 14 d post spraying.[Conclusion] Thyme oil has the highest toxicity and control effect on S.heraclei.Three agents combined with organosilicon have the obvious effect of reducing the quantity and increasing the efficiency,which has the potential for further development.

关键词： Lonicera japonica Semiaphis heraclei Plant secondary metabolites Additive