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Stool-based dna testing,a new noninvasive method for colorectal cancer screening,the first report from Iran

World Journal of Gastroenterology 2007年第10期13卷 1528-1533页

作者： Mohammad Reza Abbaszadegan Alireza Tavasoli Arash Velayati Hamid Reza Sima Hassan Vosooghinia Mehdi Farzadnia Hamid Asadzedeh Mehran Gholamin Ezzat Dadkhah Azadeh Aarabi Mohammad Reza Abbaszadegan Division of Human GeneticsImmunology Research CenterBu-Ali Research InstituteMUMSMashhadIran Department of Surgery Ghaem HospitalMashhad University of Medical SciencesMashhadIran Department of Internal Medicine Imam Reza HospitalMUMSMashhadIran Department of Internal Medicine Ghaem HospitalMUMSMashhadIran Department of Pathology Imam Reza HospitalMUMSMashhadIran

AIM： To detect tumor-associated dna changes in stool samples among Iranian patients with colorectal cancer （CRC） compared to healthy individuals using BAT-26, p16 hypermethylation and long dna markers. METHODS： Stool dna was isolated from 45 subjects including 25 CRC patients and 20 healthy individuals using a new, fast and easy extraction method. long dna associated with tumor was detected using polymerase chain reaction method. Microsatellite studies were performed utilizing denaturating polyacrylamide gel to determine the instability of BAT-26. Methylation status of p16 promoter was analyzed using methylation-specific PCR （MSP）. RESULTS： The results showed a significant difference in existence of long dna （16 in patients vs 1 in controls, P 〈 0.001） and p16 （5 in patients vs none in controls, P = 0.043） in the stool samples of two groups. long dna was detected in 64% of CRC patients; whereas just one of the healthy individuals was positive for long dna. p16 methylation was found in 20% of patients and in none of healthy individuals. Instability of BATo26 was not detected in any of stool samples. CONCLUSION： We could detect colorectal cancer related genetic alterations by analyzing stool dna with a sensitivity of 64% and 20% and a specificity of 95% and 100% for long dna and p16 respectively. A non- invasive molecular stool-based dna testing can provide a screening strategy in high-risk individuals. However, additional testing on more samples is necessary from Iranian subjects to determine the exact specificity and sensitivity of these markers.

关键词： Stool dna Colorectal cancer Cancer screening long dna BAT-26 p16

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