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Evaluation of entomopathogenic Bacillus sphaericus isolated from lombok beach area against mosquito larvae

Asian Pacific Journal of Tropical Biomedicine 2016年第2期6卷 148-154页

作者： Bambang Fajar Suryadi Bagyo Yanuwiadi Tri Ardyati Suharjono Suharjono Department of Biology Faculty of Mathematics Natural Sciences Mataram University Department of Biology Faculty of Mathematics Natural Sciences Brawijaya University

Objective: To isolate, characterize and evaluate toxicity of Bacillus sphaericus(B. sphaericus) from beach area of lombok ***: Soil was collected from determined locations and suspended in sterile physiological saline water. After heat shock was applied, suspension was spread on NYSM agar medium. Colonies grown were then observed and isolated. Colony, cell morphology,and biochemical/physiological characteristics were tested and compared to B. sphaericus2362 as standard. Initial toxicity testing was done against three species of mosquito larvae(Culex quinquefasciatus, Anopheles aconitus and Aedes aegypti) and isolates that showed more than 50% larvae killing will be assayed to obtain LC_(50) and LC_(90)values within 48 *** technique were conducted to obtain 16 s r DNA amplicon for sequencing and to detect toxin-expressing genes(using multiplex PCR).Results: Twenty isolates of B. sphaericus have been collected from 20 determined locations and their characteristics were in agreement with standard B. sphaericus characteristics. Bioassay testing showed that four isolates(namely isolate MNT, SLG, TJL2 and PLG) were mildly toxic against all larvae. The rests were either low toxic or non-toxic at *** analysis showed that all four isolates were clustered with other known mildly and highly toxic strains. The multiplex PCR result showed four toxic isolates owned 1–2bands from Bin toxin genes and three bands from Mtx toxin genes, whereas 16 isolates with low to non-toxic characteristics showed only three bands from Mtx toxin ***: Four toxic isolates of B. sphaericus were isolated from beach area of lombok Island. They showed mild toxicity against larvae of three mosquito species.

关键词： Bacillus sphaericus lombok Island Mosquito larvae Culex quinquefasciatus Anopheles aconitus Aedes aegypti

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Parasitic nematodes of amphibians from lombok Island,Indonesia with description of Camallanus senaruensis *** Meteterakis lombokensis ***.

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Journal of Coastal Life Medicine 2016年第9期4卷 708-713页

作者： Endang Purwaningsih Kartika Dewi Herjuno Ari Nugroho Zoological Division of Research Center for Biology-Indonesian Institute of Sciences Widyasatwaloka BuildingJl.Raya Jakarta Bogor Km 46Cibinong 16911Indonesia

Objective:To determine and describe the nematode species of amphibians collected from lombok Island,West Nusa Tenggara,***:The materials examined were found in the intestines of twenty-four Fejervarya cancrivora(***),sixteen Fejervarya verruculosa,six Duttaphyrnus melanostictus(***)from Senaru and Gangga District,lombok Island on April *** amphibian hosts were collected by *** observing the nematodes,the hosts were anesthesized to death with *** ventral of the host was opened by longitudinal incision and the internal organs were removed,placed separately in the Petri-dish and then examined under a dissecting *** nematodes found were fixed with warm 70%*** specimens for light microscope observation were cleared in glycerol and mounted in the same solution,and for the SEM Jeol scanning electron microscope 5310 low vacuum were re-fixed in caccodylate buffer and glutaraldehyde,dehydrated through a graded series of alcohol and vacuum-dried using TAITEC Vacuum-96Nitrogen,prior to attaching to stubs with double sided cello-tape,coated with gold 400?thickness in an Eico I-B2 ion *** were made with the aid of a drawing tube attached to a Nikon compound *** were given in micrometers(μm)as the average,followed by the range in parentheses,unless otherwise ***:Two new species of nematodes were found and described herein:Camallanus senaruensis ***.,and Meteterakis lombokensis ***.,parasitic in the intestine of *** and ***,*** differs from other congeners in having a bluntly rounded tip of tail in the male and female,the structure of trident and having teeth in the buccal *** lombokensis differs from other previously described species in having no vulval flap,has a strongly widened proximal end of spicules,forming a cup shaped,and the number of caudal *** species found are Meteterakis

关键词： Nematode Camallanus senaruensis sp.nov. Meteterakis lombokensis sp.nov. Amphibian lombok Island

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The Impact of Promoting Tourist Destinations after Earthquake in lombok

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Journal of Tourism and Hospitality Management 2019年第4期7卷 180-187页

作者： Farid Said Hamsu Hanafi Surayyal Hizmi Zulkifli Harahap lombok Tourism Polytechnic Lombok TengahNusa Tenggara BaratIndonesia Palembang Polytechnic of Tourism Kota PalembangSouth SumatraIndonesia

lombok Island has been rocked by multiple powerful earthquakes since July 2018 and it remained hundreds of *** by August 2018 more than 400 people have lost their *** tremendous disaster not only affected to damage infrastructures and trauma,but also affected tourism market of lombok as one of the priority areas for the development of tourism market in *** is reported that tourism market in lombok has decreased dramatically after the earthquake from 1.3 million to 250 thousands *** reduction of tourists visiting lombok was not only in the affected destinations but also in unaffected *** improve the number of tourists visiting lombok,promoting several unaffected tourist destinations was carried out after 4 months of earthquake through sales missions and fam *** missions were conducted in Philippine,Singapore,UK,Thailand,and several main cities in Indonesia,while fam trips were conducted in several affected destinations in *** results showed that the buyer from sales missions reached 475 pax and potential transaction for fam trip reached up to 108 billion rupiah within a month after the *** of tourist destinations in lombok after disaster is then continued with promoting affected destination in 2019,such as Gili islands and Senggigi and *** resulted in improvement of numbers of hotel occupation for around 10%within six *** of tourist destinations was not only proposed to improve the number of tourists visiting lombok but also to improve and promote safety procedures during visiting affected *** was proved by existence of resemble points,safety briefing during event in all hotels and convention halls in lombok,as well as insurance cover in several tour package on the travel ***,in order to optimize tourism market,government should also contribute to providing better infrastructures and workshop for appropriate safety procedures to people

关键词： earthquake lombok destinations promotion safety

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