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Comparative analysis of cytochrome P450-like genes from locusta migratoria manilensis： expression profiling and response to insecticide exposure

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Insect Science 2012年第1期19卷 75-85页

作者： Yan-Qiong Guo Jian-Zhen Zhang Mei-Ling Yang Liang-Zhen Yan Kun Yan Zhu Ya-Ping Guo En-Bo Ma Institute of Appfied Biology Shanxi UniversityTaiyuan College of Agriculture Shanxi Agricultural UniversityTaiguShanxi ProvinceChina Department of Entomology Kansas State UniversityManhattanKansasUSA

The cytochrome P450 monooxygenase （cytochrome P450） gene superfamily comprises many genes that may be involved in the biotransformations of pesticides and other xenobiotics. To date, very little is known about cytochrome P450 genes in the oriental migratory locust, locusta migratoria manilensis. In this study, we carried out a genomewide analysis ofcytochrome P450 genes of the locust to identify putative cytochrome P450 genes and characterize their expression responses to insecticide exposures. We identified 15 cytochrome P450-1ike genes from a locust expressed sequence tag database （LocustDB）. Reverse transcription polymerase chain reaction （RT-PCR） analysis showed that most cytochrome P450-1ike genes displayed different tissue and developmental stage expression patterns. However, most of them were predominantly expressed in the midgnt, gastric caeca, fatbodies, and/or hindgut. Biochemical analysis showed that cytochrome P450 was differentially affected by three different insecticides. Deltamethrin caused significant inductions in 12 h at LD30 （dose to kill 30% of the tested individuals） in the nymphs, whereas malathion and carbaryl did not have significant effect on cytochrome P450 enzyme activity. Further RT-PCR analysis Showed significant increases of transcriptions of several cytochrome P450 genes in deltamethrin-treated locusts. Thus, the increased cytochrome P450 enzyme activity is likely due to increased transcriptions of multiple cytochrome P450 genes in response to deltamethrin exposure. These results are expected to help us better understand the interactions between insecticides and major detoxification enzymes, and possible changes of the susceptibility to other insecticides in deltamethrin-treated insects at various molecular levels.

关键词： cytochrome P450 monooxygenase deltamethrin gene expression profiling insecticide locusta migratoria manilensis

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Optimization of parental RNAi conditions for hunchback gene in locusta migratoria manilensis （Meyen）

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Insect Science 2010年第1期17卷 1-6页

作者： Zheng-Bo He Yue-Qing Cao Yu-Xian Xia Genetic Engineering Research Center College of Bioengineering Chongqing University Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission Chongqing China

An investigation on the optimization of parental RNA interference （RNAi） conditions for hunchback （hb） gene in locusta migratoria manilensis （Meyen） was conducted. Double stranded RNA （dsRNA） corresponding to hb gene was injected into haemocoel of female adults ofL. migratoria manilensis. Embryos developed from the eggs laid by the injected adults on the 7th day after eclosion showed observable effects of RNAi for hb. The silencing effect after delivery treatment of dsRNA for hb gene was maintained for more than 21 days. A significant decrease of hb transcripts was further confirmed by Northern blot analysis. The dose of dsRNA/insect at 2μg could trigger 96.9% RNAi effects, while silencing appeared to have no dependence on the size of dsRNA. Results suggest that parental RNAi could be employed to efficiently identify the developmental gene functions in L. migratoria manilensis.

关键词： hunchback locusta migratoria manilensis parental RNA interference

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Effects of Glutamate and Na^+ on the Development and Enzyme Activity of the Oriental Migratory Locust,locusta migratoria manilensis(Meyen) in Successive Generations

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Journal of Integrative Agriculture 2014年第4期13卷 819-826页

作者： ZHAO Xia JIA Miao WANG Lei CAO Guang-chun ZHANG Ze-hua State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Plant Protection Shenyang Agricultural University

Rapid and mass rearing oflocusta migratoria manilensis is an urgent need to meet the increasing demand for food of people. In this study, the effects of four artificial feeds on the development, reproduction and the activities of detoxification and protective enzymes of L. migratoria manilensis in three successive generations were investigated. The results showed that sucrose and monosodium glutamate （MSG） significantly increased the net reproductive rate （R0） and the intrinsic growth rate （rm） ofL. migratoria manilensis, but sodium chloride （0.17%） suppressed this increase. Furthermore, the artificial feed with sucrose and monosodium glutamate increased the activities of esterase （EST）, acetylcholinesterase （ACHE）, glutathione-S- transferase （GST）, multi-function oxidase （MFO）, phenol oxidase （PO）, catalase （CAT） and peroxidase （POD）, but inhibited the activity of superoxide dismutase （SOD）. However, sodium chloride （0.17%） increased the activities of EST, ACHE, CAT and SOD, and inhibited the activities of MFO, GST, PO and POD. Correlation analysis found that the increasing of PO activity and the decreasing of SOD activities were significantly related with the increasing of the intrinsic growth rate （rm）. The above results indicated that sucrose and monosodium glutamate could promote the development and reproduction of L. migratoria manilensis, but Na＋ inhibit such promotion with the concentration above 0.2%. The activities of PO and SOD can be used as biochemical standard to assess the effect of artificial feed.

关键词： locusta migratoria manilensis artificial feed intrinsic rate phenol oxidase superoxide dismutase

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Identification and characterization of a gene encoding a UBX domain-containing protein in the migratory locust, locusta migratoria manilensis

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Insect Science 2013年第4期20卷 497-504页

作者： Zheng-Bo He Yu Xie Feng-Ling Si Bin Chen Institute of Entomology and Molecular Biology Chongqing Key Laboratory of Animal Biology College of Life Sciences Chongqing NormalUniversity Chongqing China

Ubiquitin regulatory X （UBX） domain-containing proteins are believed to func- tion as cofactors for p97/CDC48, an adenosine triphosphatase shown to be involved in mul- tiple cellular processes. In the present study, a full-length complementary DNA （cDNA） of UBX domain-containing gene, termed LmUBX1, was cloned from locusta migratoria manilensis and characterized, using random amplification of cDNA ends polymerase chain reaction （RACE PCR）, sequence analysis and quantitative real-time PCR. LmUBX1, 1 600 bp in length, is predicted to encode a 446-amino acid protein with a predicted molec- ular weight of 51.18 kDa that contains a central PUB domain and a carboxy-terminal UBX domain. Homology analysis revealed that LmUBX1 has higher similarity to the known UBX domain-containing proteins from insects than from other species. Moreover, based on sequence characteristics and phylogenetic relationships, it is suggested that LmUBX1 can be classified into the UBXD1 subfamily. Expression analysis founded that LmUBX1 exhibited significant expression variations at different developmental stages and in differ- ent tissues, suggesting that the expression of LmUBX1 was highly regulated. Interestingly, its messenger RNA transcript was more abundant in ovary and testis than in other tissues examined, suggesting that it may have more important roles in the reproductive system. In addition, LmUBX1 was differentially expressed in gregarious and solitary locusts and was significantly up-regulated in third and fifth instars of gregarious locusts, implying that LmUBX1 was also likely involved in the phase polyphenisms in L. migratoria manilen- sis. To our knowledge, this is the first report of cloning of a full-length cDNA of UBX domain-containing gene from L. migratoria manilensis.

关键词： cloning expression locusta migratoria manilensis UBX domain-containing protein

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Expression and Characterization of a Sigma-Class Glutathione S-transferase of the Oriental Migratory Locust, locusta migratoria manilensis (Meyen)

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Agricultural Sciences in China 2011年第10期10卷 1570-1576页

作者： JIA Miao QIN Guo-hua LIU Ting ZHANG Jian-zhen ZHANG Xue-yao ZHU Kun-yan GUO Ya-ping MA En-bo Researeh Institute of Applied Biology Shanxi University Taiyuan 030006 P.R. China College of Environmental Science and Resource Shanxi University Taiyuan 030006 P.R.China Department of Entomology 123 Waters Hall Kansas State University Manhattan KS 66506 USA School of Life Science Shanxi University Taiyuan 030006 P.R. China

A cDNA encoding a sigma-class glutathione S-transferase of the locust, locusta migratoria manilensis （LmGSTs1）, was cloned by reverse transcriptase-polymerase chain reaction. The 830 bp-long cDNA encoded a 615 bp open reading frame （204 amino acid polypeptide）, which exhibited the structural motif and domain organization characteristic of GST sigma-class. It revealed 59, 57, 57, and 56% identities to sigma-class GSTs from Blattella germanica, Gryllotalpa orientalis, Nasonia vitripennis, and Pediculus humanus corporis, respectively. A recombinant protein （LmGSTs1） was functionally expressed in Escherichia coli cells in a soluble form and purified to homogeneity. LmGSTs1 was able to catalyze the biotranslation of glutathione with 1-chloro-2,4-dinitrobenzene, a model substrate for GSTs, as well as with p-nitro-benzyl chloride. Its optimal activity was observed at pH 8.0 and at 30℃. Incubation for 30 min at temperatures below 50℃ scarcely affected the activity. The I50 of reactive blue （RB） was 18.5 μmol L-1. In the presence of 0.05 mmol L-1 ethacrynic acid （ECA）, LmGSTs1 showed （81±3）% of the original activities.

关键词： glutathione S-transferase GST locust locusta migratoria manilensis Orthoptera sigma-class

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东亚飞蝗locusta migratoria manilensis(Meyen)的骨骼肌肉系统Ⅱ.胸部

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昆虫学报 1964年第4期 510-535页

作者：虞佩玉陆近仁中国科学院动物研究所北京农业大学

本文是东亚飞蝗骨骼肌肉系统研究的第二部分,详细记述和讨论胸部的骨骼及肌肉,包括足及翅;并且与非洲飞蝗(locusta migratoria migratorioides(Reiche et Fairmaire))、卡罗来纳蝗(Dissosteiracarolina(Linnaeus))及沙漠蝗(Schistocera gregaria(Forskal))等作了比较。根据第五龄蝗蝻翅芽的气管分布及成虫翅脉基端与翅关节片的关联,我们对于前、后翅的肘脉及臀脉等作了探讨。我们看到有六对肌肉为以往有关蝗虫解剖的文献中所没有报导过。它们是:前足基节前旋肌(77)、前足转节腹提肌(83)、中足转节第二背提肌(117)、中足转节前腹提肌(118)、中足转节后腹提肌(119)、后足转节背缘后提肌(150)。我俩认为前足基节前旋肌的发现是很有意义的,因为这一肌肉对于前足的活动是非常重要的。胸部肌肉的总数是103对,其中38对属于前胸,35对属于中胸,和30对属于后胸。文章的最后部分讨论了16对肌肉,其中9对属于中胸和7对属于后胸。作者将这些肌肉统称为初羽肌,因为这些肌肉仅出现于各龄蝗蝻及初羽化的成虫,而在成虫的发育过程中很快消失。

关键词：腋片基部前翅肘脉 Meyen locusta migratoria manilensis 后胸背板后上侧片基节翅基片翅关节后翅腹内脊前盾片东亚飞蝗大头蚂炸胸部躯干骨骼肌肉

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东亚飞蝗locusta migratoria manilensis(Meyen)二型生物学特性的初步研究

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昆虫学报 1964年第3期 329-338页

作者：黄亮文马世骏中国科学院动物研究所

本文是研究东亚飞蝗二型生物学特性的一部分报导,目的在于通过二型生物学特性的比较,阐明二型在种群数量变动上的某些特点。实验结果指出:1.散居型飞蝗的发育速度大于群居型。低密度虫群的成活率亦高于高密度虫群。2.散居型成虫产卵量... 详细信息

本文是研究东亚飞蝗二型生物学特性的一部分报导,目的在于通过二型生物学特性的比较,阐明二型在种群数量变动上的某些特点。实验结果指出:1.散居型飞蝗的发育速度大于群居型。低密度虫群的成活率亦高于高密度虫群。2.散居型成虫产卵量高于群居型,其卵小管数目也比后者稍多。3.若以食物吸收量与取食量的干重比例为指标,则散居型食物利用系数低于群居型。4.群居型体内水分含量比散居型低,但脂肪含量则比散居型高,实测结果前者的耐寒性比后者略强。此外,本文还简单讨论了二型的上述特性差异在飞蝗猖獗学上的意义。

关键词：散居型发育速度脂肪含量群居型东亚飞蝗大头蚂炸 locusta migratoria manilensis Meyen 生物学特性

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高温低湿对东亚飞蝗(locusta migratoria manilensis(Meyen))一龄蝻生长的影响及其实验方法

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昆虫知识 1964年第1期 30-32页

作者：吴亚中国科学院动物研究所

东亚飞蝗大发生与干燥气候有直接的联系。干燥对飞蝗的生长及发育究竟有什么作用或影响,过去几年在蝗卵发育与湿度及土壤含水量的关系方面已做了许多研究。在蝻与成虫方面,由于在通风条件下的低湿控制技术未获解决,室内试验未能较好的开... 详细信息

东亚飞蝗大发生与干燥气候有直接的联系。干燥对飞蝗的生长及发育究竟有什么作用或影响,过去几年在蝗卵发育与湿度及土壤含水量的关系方面已做了许多研究。在蝻与成虫方面,由于在通风条件下的低湿控制技术未获解决,室内试验未能较好的开展,因而累积的资料不多。本文介绍了一种在保持通气和正常饲养状况下的低湿控制装置,以及用这种装置进行试验所获得的初步结果,以供有关同志参考。

关键词：东亚飞蝗活率大头蚂炸发育速率 locusta migratoria manilensis Meyen

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东亚飞蝗(locusta migratoria manilensis(Meyen))的骨骼肌肉系流 Ⅰ.头部

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昆虫学报 1957年第1期 1-19页

作者：陆近仁虞佩玉北京农业大学、中国科学院

前言蝗科的种类虽然是常被用来作外部形态的比较研究,但是关于整个骨胳和肌肉系统的研究却并不很多。Snodgrass在他的一系列的报告(1927,1928,1929,1935,1937)中,曾经对美洲的卡罗来纳蝗(Dissosteira carolina(Linnaeus))的骨胳肌肉系... 详细信息

前言蝗科的种类虽然是常被用来作外部形态的比较研究,但是关于整个骨胳和肌肉系统的研究却并不很多。Snodgrass在他的一系列的报告(1927,1928,1929,1935,1937)中,曾经对美洲的卡罗来纳蝗(Dissosteira carolina(Linnaeus))的骨胳肌肉系统作了比较详细的叙述。张光朔(Chang,1931,1933)曾经作了车蝗(Gastrimargus(Oedaleus)

关键词：着生头部解剖部位东亚飞蝗大头蚂炸 Meyen locusta migratoria manilensis

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东亚飞蝗locusta migratoria manilensis(Meyen)两型形态比较初步研究

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昆虫学报 1965年第6期 603-609页

作者：高慰曾中国科学院动物研究所

本文对东亚飞蝗两型的卵、蝻及成虫的形态以及成虫的生殖、消化两系统各器官的长度进行了比较,试图明确两型在形态与解剖方面的差异。研究方法所用材料,除室内试验用卵系来自山东德州内涝蝗区(后经在北京室内饲养至第三代)外,其他省... 详细信息

本文对东亚飞蝗两型的卵、蝻及成虫的形态以及成虫的生殖、消化两系统各器官的长度进行了比较,试图明确两型在形态与解剖方面的差异。研究方法所用材料,除室内试验用卵系来自山东德州内涝蝗区(后经在北京室内饲养至第三代)外,其他省采自河南浚县蝗区。群居型蝻及成虫均系自野外采得一、二龄蝻,置于田间铁纱笼内(笼容积为33×33×33立方公分)饲养获得。每笼放幼蝻300头。散居型蝻

关键词：东亚飞蝗大头蚂炸群居型散居型 Meyen locusta migratoria manilensis 两型形态比较

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