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Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

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World Journal of Gastroenterology 2008年第5期14卷 776-781页

作者： Bin Yan An-Chun Cheng Ming-Shu Wang Shu-Xuan Deng Zhen-Hua Zhang Nian-Chun Yin Ping Cao Sheng-Yan Cao Avian Disease Research Center Collage of Veterinary Medicine of Sichuan Agriculture University Yaan 625014 Sichuan Province China College of Life Science and Technology of Southwest University for Nationalities Chengdu 610041 Sichuan Province China Key Laboratory of Animal Disease and Human Health of Sichuan Province College of Veterinary Medicine of Sichuan Agriculture University Yaan 625014 Sichuan Province China

AIM： To detect Salmonella enteritidis （S. enteritidis） in paraffin slices and antigen location in infected duck tissues. METHODS： The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method （IFA） was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis. RESULTS： The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.CONCLUSION： IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.

关键词： Indirect immuno-fluorescent antibody staining Salrnonella enteritidis Paraffin tissues Detection localization of antigens

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Application of indirect immunofluorescent staining method for detection Salmonella Enteritidis in paraffin slices of and antigen location of duck

Application of indirect immunofluorescent staining method fo...

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第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Bin YAN An-Chun CHENG Ming-Shu WANG Shu-Xuan DEN Bin YAN Shu-Xuan DEN (1.Avain Disease Research Center,Collage of Animal Science and Veterinary Medicine of Sichuan Agriculture University 2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Animal Science and Veterinary Medicine of Sichuan Agriculture University,Yaan,625014,Sichuan Province,China.)

Detect Salmonella Enteritidis in paraffin slices of and antigen location organ tissues of *** rabbits were immunized with purified bacillus to obtain SE-specific antibody,which were then extracted by caprylic—ammonium sulphate method,purified through High-Q *** indirect immuno-fluorescent staining method(IFA) was established to detect the *** antigen in paraffin *** *** in each organ tissues of ducklings experimentally infected with *** Gland of Garder、Heart、Kidney、Spleen、Liver、Brain and Ileum、Jejunum、Bursa of Fabricins from *** experimentally infected ducklings were positive or strongly positive,and the *** antigen mainly distributes in the infected cell *** is a intuitionistic、sensitive and specific method in detecting *** antigen in paraffin wax slice,and it’s a good method in diagnosis and antigen location of *** also conculd that The Gland of Garder、Heart、Kidney、Spleen、*** intestine are target organs in *** infections of duck.

关键词： Indirect Immuno-Fluorescent Antibody Plan Salmonella Enteritidis of Duck Detection localization of antigens

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