检索结果-南通市图书馆

Effects of lizhong Tang on cultured mouse small intestine interstitial cells of Cajal

World Journal of Gastroenterology 2013年第14期19卷 2249-2255页

作者： Min Woo Hwang Jung Nam Kim Ho Jun Song Bora Lim Young Kyu Kwon Byung Joo Kim Department of Sasang Constitutional Medicine College of Korean MedicineKyung-hee University School of Korean Medicine Pusan National University

AIM:To investigate the effects of lizhong Tang,an herbal product used in traditional Chinese medicine,on mouse small intestine interstitial cells of Cajal(ICCs).METHODS:Enzymatic digestions were used to dissociate ICCs from mouse small intestine tissues.The ICCs were morphologically distinct from other cell types in culture and were identified using phase contrast microscopy after verification with anti c-kit antibody.A whole-cell patch-clamp configuration was used to record potentials(current clamp) from cultured ICCs.All of the experiments were performed at 30-32 ℃.RESULTS:ICCs generated pacemaker potentials,and lizhong Tang produced membrane depolarization in current-clamp mode.The application of flufenamic acid(a nonselective cation channel blocker) abolished the generation of pacemaker potentials by lizhong Tang.Pretreatment with thapsigargin(a Ca 2+-ATPase inhibi-tor in the endoplasmic reticulum) also abolished the generation of pacemaker potentials by lizhong Tang.However,pacemaker potentials were completely abolished in the presence of an external Ca 2+-free solution,and under this condition,lizhong Tang induced membrane depolarizations.Furthermore,When GDPβ-S(1 mmol/L) was in the pipette solution,lizhong Tang still induced membrane depolarizations.In addition,membrane depolarizations were not inhibited by chelerythrine or calphostin C,which are protein kinase C inhibitors,but were inhibited by U-73122,an active phospholipase C inhibitors.CONCLUSION:These results suggest that lizhong Tang might affect gastrointestinal motility by modulating pacemaker activity in interstitial cells of Cajal.

关键词： Interstitial cells of Cajal lizhong Tang Motility Gastrointestinal tract Whole-cell patch clamp configuration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of lizhong Tang on gastrointestinal motility in mice

引用

World Journal of Gastroenterology 2016年第34期22卷 7778-7786页

作者： Min Cheol Lee Wooram Ha Jinhyeong Park Junghoon Kim Yunjin Jung Byung Joo Kim Division of Longevity and Biofunctional Medicine Healthy Aging Korean Medical Research CenterSchool of Korean MedicinePusan National UniversityYangsan 50612South Kerea Division of Pharmacology School of Korean MedicinePusan National University Yangsan 50612South Kerea College of Pharmacy Pusan National UniversityBusan 46241 South Korea

AIM To investigate the effects of lizhong Tang,a traditional Chinese medicine formula,on gastrointestinal motility in mice.METHODS The in vivo effects of lizhong Tang on GI motility were investigated by measuring the intestinal transit rates(ITRs) and gastric emptying(GE) values in normal mice and in mice with experimentally induced GI motility dysfunction(GMD).RESULTS In normal ICR mice,the ITR and GE values were significantly and dose-dependently increased by lizhong Tang(ITR values: 54.4% ± 1.9% vs 65.2% ± 1.8%,P lizhong Tang and 54.4% ± 1.9% vs 83.8% ± 1.9%,P lizhong Tang; GE values: 60.7% ± 1.9% vs 66.8% ± 2.1%,P lizhong Tang and 60.7% ± 1.9% vs 72.5% ± 1.7%,P lizhong Tang). The ITRs of the GMD mice were significantly reduced compared with those of the normal mice,which were significantly and dose-dependently reversed by lizhong Tang. Additionally,in loperamide- and cisplatin-induced models of GE delay,lizhong Tang administration reversed the GE deficits.CONCLUSION These results suggest that lizhong Tang may be a novel candidate for development as a prokinetic treatment for the GI tract.

关键词： lizhong Tang Gastrointestinal disorders Motility Intestinal transit rate Gastric emptying

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

lizhong Group Invests RMB 1 Billion To Build A Production Base for Aluminum Wheels And Aluminium Alloy Materials

引用

China Nonferrous Metals Monthly 2019年第12期 6-7页

On October 10,2019,lizhong Group and Wuhan Caidian District People’s Government held a signing ceremony,marking the official launching of aluminium alloy wheels and auto lightweight aluminium alloy material project o... 详细信息

关键词： lizhong Group Aluminium Alloy Materials Materials

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Roles of three classic TCM formulae in inhibiting gastric cancer growth in mice

引用

Journal of Traditional Chinese Medical Sciences 2017年第1期4卷 65-70页

作者： Liyun Yue Changle Zhu Chong Gao Chong Wang Xinyi Chen Tongde Tian Department of Hematology and Oncology Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineBeijing 100700China Integrated TCM&Western Medicine Department Henan Cancer HospitalZhengzhou 450000China

Objective:To explore the effects of three classic traditional Chinese medicine (TCM) formulae,Dahuang Huanglian Xiexin Decoction (DHXD),Fuzi lizhong pills (FLP) and Liushen pills (LSP),in suppressing gastric cancer growth in mice and provide evidence for their clinical applications.Methods:MGC-803 cells (5 × 106 per mouse) were injected into the subcutaneous tissues of the neck and back of nude mice.One week later,the mice were divided into three batches (9 total groups) based on tumor size and a random number table.Within each batch,the mice were subdivided into control,low-and high-dose groups for the three classic formulae.After 21-d treatment,the mice were observed for their general conditions,and then sacrificed.Tumor tissues were harvested and weighed,and the tumor inhibition rate was calculated.Results:Compared with the control group,body weight in the low-and high-dose DHXD and FLP groups showed no significant change (P >.05),while the high-dose LSP group had significantly lower body weight (P =.049).Compared with the control group,the low-and high-dose DHXD groups showed no significant inhibitory effect on xenograft tumor growth (P >.05);lowdose FLP showed an inhibitory effect on tumor growth (P =.039),while high-dose LSP had a more significant effect (P =.004).Conclusions:DHXD did not inhibit xenograft tumor growth in mice.In contrast,low-dose FLP had a measurable inhibitory effect,and high-dose LSP more significantly suppressed xenograft tumor growth in mice.

关键词： Dahuang Huanglian Xiexin Decoction Fuzi lizhong pills Liushen pills Gastric cancer Tumor inhibition rate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：